• Korean Journal of Weed Science
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• v.6 no.2
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• pp.130-133
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• 1986

Glyphosate and 2,4-D were introduced into the vascular systems of Robinia pseudo-acacia L. (3-5cm diameter) with a syringe-type pressure injector in May, 1985. Robinia pseudo-acacia L. were completely controlled by 3 or 6 ml of 3050ppm glyphosate per tree and 6ml of 1000ppm 2,4-D per tree. Herbicidal efficacy was more effective by 6m1 of combined solutions of 1525ppm glyphosate and 500ppm 2,4-D per tree.

• The Korean Journal of Pesticide Science
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• v.13 no.4
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• pp.267-274
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• 2009

This study was carried out to select effective preventive pesticides against pine wilt disease caused by pinewood nematode (PWN), Bursaphelenchus xylophilus on trunk injection. 1,000 fold aquatic solution of abamectin 1.8% EC and emamectin benzoate 2.15% EC were lower mortality (7.3% and 8.3% respectively) against PWN on 1 day after treatment. However effects of abamectin 1.8% EC, emamectin benzoate 2.15% EC, fosthiazate 30% SL and fenitrothion 30% SL were inhibited the reproduction of PWN over 99.6% in Botrytis cineria media. Effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $10\;m{\ell}$ per 10 cm in diameter of breast height (DBH) on mortality of Japanese black pine, Pinus thungergii by inoculated PWN was 0% and 3.3%, respectively at the applied year however when injection of fosthiazate 30% SL were treated with the rate of $5\;m{\ell}$ per 10 cm tree DBH, mortality of tree was 63.3%. Abamectin 1.8% EC and emamectin benzoate 2.15% EC was showed high preventive efficacy representing >90% against PWN at the following year. PWN preventing efficacy of trunk injection was lower in naturally occurred area (mortality of pine tree in control was 11.7% at the first year) of PWN than artificially infected site (mortality of pine tree in control was >76.7% at the first year), PWN preventing efficacy of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $10\;m{\ell}$ per 10 cm in DBH was 91.5% and 82.9%, respectively, at the applied year and 89.5% and 82.6% respectively at the following year in PWN naturally occurred site. Control efficacy by trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC was more higher in 10 fold dilution with 10 fold high amount of aquatic solution than no dilution with 10 fold less amount of aquatic solution. The preventive effect of trunk injection of abamectin 1.8% EC and emamectin benzoate 2.15% EC at the rate of $5\;m{\ell}$ per 10 cm in DBH was showed 100% at the applied year in PWN inoculated tree.

• The Korean Journal of Pesticide Science
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• v.19 no.4
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• pp.440-449
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• 2015

Pine wood nematode (PWN), Bursaphelenchus xylophilus is one of the most serious pests of pine tree. Trunk injection of some nematicides in tree is well known as an effective control method. However there are some limitating factors which hindering the efficacy of trunk injection in field or potted tree. In this study we suggested easy and useful alternative screening methods of nematicides against PWN. Reproduction of PWN was influenced by tree twig moisture (high reproduction in high moisture trig) and paraffin coating was 78.6% reduced moisture loss in tested twig. There were no reproduction different in up and down site from infection site of twig at 1 month after inoculation of PWN and also distance (5 and 10 cm) from inoculation site of PWN did not influence the reproduction of PWN. Numbers of reproduced PWN were higher with decreasing diameter of twig. Numbers of reproduced PWN were similar to P. thunbergii and P. densiflora. However reproduction was increased depending on high inoculation density and longer propagation period. When inoculation of PWN on cut twig injected with emamectin benzoate 2.15% EC and morantel tartrate 8% SL in trunk of Pinus thunbergii in the field, PWN number were significantly reduced than untreated control. We suggest this screening method for PWN control agents.

• 건강소식
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• v.34 no.4
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• pp.28-31
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• 2010

오리숲길 소나무가 하늘을 가렸다. 상쾌한 솔향기에 온몸이 싱그러워진다. 걷다가 만난 황톳길을 맨발로 걷는다. 오리숲길 중간에 있는 일주문을 지나 자연관찰로로 접어든다. 그 길에 조릿대와 생강나무, 전나무, 신갈나무, 소나무 등이 빽빽하게 들어섰다. 흙길이 부드럽다. 법주사에서 마음을 씻어내고 되돌아 나오는 길, 속리산 자락에서 나는 산나물 산약초로 만든 비빔밥으로 풋풋한 봄 맛을 느껴볼 일이다.

• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.56-65
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• 2016

Pine wood nematode, Bursaphlenchus xylophilus is a fatal damaging pest, infecting pinewood trees and is one of the serious forest pest in Korea. Trunk injection is one of the most effective method to protect the live pine trees from nematode infection. The aim of this research was to reveal the efficacy of the nematicidal materials from 46 Kyrgyzstan, 88 Vietnam plant materials and 21 active materials (${\alpha}$-terpinene, ${\alpha}$-pinene, ${\beta}$-thujaplicin, cinnamaldehyde, eugenol, emodin, geraniol, limonone, methyl palmitate, matrine, myrcene, methyl gallate, nicotine, quassin, resveratrol, rotenone, thymol, thujaplicin, terthiophene, tuberstemonine, ${\gamma}$-terpinene) isolated from plants for using as trunk injection agents. Eclipta prostrata extract was highly effective against pine wood nematode in plant extracts and terthiophene has the best nematicidal activity from active materials isolated from plants. Foliar spray of terthiophene on Japanese black pine (Pinus thunbergii) tree reduced proliferation of inoculated pine wood nematode at 22 days after treatment, however no efficacy was found over 22 days. Eclipta prostrata extract and terthiophene can be used as nematicide for further nematicidal efficacy test against any other plant parasitic nematodes and trunk injection efficacy against pine wood nematode.

• Research in Plant Disease
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• v.26 no.1
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• pp.19-28
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• 2020

Jujube witches' broom (JWB) disease is a high-risk plant disease that occurred on and devastated jujube orchards throughout South Korea in the 1950s. Recently, as the jujube cultivation area has been significantly increased nationwide, we investigated the disease in jujube orchards in the Boeun region to obtain basic information for proactively evaluating the risk of JWB outbreaks from 2017 to 2019. A field trial was conducted to evaluate the effect of the trunk injection of Sungbocycline (a. i. oxytetracycline calcium alkyl trimethyl ammonium 17%, OTC). The incidence of JWB disease was very low in Boeun, but it was gradually increased mainly in the area and on the farms where it had occurred previously. The trunk injection with OTC was most effective in the early stages of growth. However, even though the trees were treated with OTC, disease symptoms appeared in other healthy branches in severely affected trees. When the tree was first injected with OTC in June, the control efficacy of second injection was higher one month after the first injection than five months. These results suggest that early diagnosis based on various symptoms and early control based on them can be very effective in preventing JWB disease in advance and increasing control efficacy.

• Research in Plant Disease
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• v.20 no.4
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• pp.295-298
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• 2014

Oak wilt caused by Raffaelea quercus-mongolicae was first noticed in South Korea in 2004 and, ever since, its distribution and damage have been increasing. To screen a fungicide effective for oak wilt control by tree injection, laboratory and field experiments were conducted. Ten fungicides and one antibiotic were examined in vivo for their effectiveness in restricting the growth of R. quercus-mongolicae and R. quercivora (Japanese oak wilt pathogen) isolates. To the Korean isolates of R. quercus-mongolicae, chlorothalonil showed the highest fungicidal effects, followed by benomyl and propiconazole. To the Japanese one, propiconazole was highest in the fungicidal effectiveness, followed by benomyl and bitertanol. Propiconazole was selected for field-testing of its control efficacy because it showed good fungicidal effects in vitro and systemic activity. The control efficacy in the field was 87.5% in the first year of injection and 66.7% in the second year, indicating the fungicidal effects last at least over one year.

• Korean Journal of Environment and Ecology
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• v.19 no.2
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• pp.75-82
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• 2005

To investigate the vegetation structure of the region from Dongchanggyo to Deogjusa in Woraksan National Park, 23 plots$(400m^2)$ set up with random sampling method were surveyed. Three groups Quercus mongolica-pinus densiflora community, Pinus densiflora-Quercus molica community, Mixed Broad-leaved community were classified by cluster analysis. Quercus mongolica, pinus densiflora, Quercus serrata and Fraxinus rhynchophyllai were found as a major woody plant species in Woraksan National Park region. In this area, Quercus mongolica and Pinus densiflora were dominated partially. In the future, the importance percentage of Pinus densiflora might be decreased, but those of Quercus mongolica, Quercus serrata and Fraxinus rhynchophyllai might be increased. High positive correlations were proved between Acer pic tum and Corylus heterophylla, Betula schimidtii; Acer pseudosieboldianum, Cornus controversa ; Stephanandra incisa and Symplocos sawafutagi, Lespedeza maximowixzii ; Symplocos sawafutagi and Fraxinus rhynchophyllai, Lespedeza maximowixzii; Fraxinus rhynchophyllai and Acer pseudosieboldianum, Lespedeza maximowixzii; Quercus variabilis and Fraxinus sieboldiana; Corylus heterophylla, and Rhododendron schlippenbachii; Acer pseudo sieboldianum and Lespedeza maximowixzii, and relatively high negative corrlations was proved between Quercus serrata and Betula schimidtii; Quercus mongolica and Quercus serrata; Corylus heterophylla and Lindera erythrocarpa. Species diversity(H') of investigated groups were ranged from $1.2393\~1.3674$ and it was relatively high compared to those of the ridge area of other national parks.

• Proceedings of the KAIS Fall Conference
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• 2011.12a
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• pp.322-325
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• 2011

본 연구는 넓은잎 딱총나무 잎으로부터 유도된 캘러스의 형태학적 특성과 효능에 관한 것이다. 딱총나무에서 유도된 캘러스 내의 세포들을 촬영한 표면주사현미경 사진에서 분열능이 높은 세포의 특성을 보여주었다. 유도된 넓은잎 딱총나무 캘러스를 생물반응기 내에서 배양시켜 얻은 딱총나무 식물세포배양 추출물은 DPPH 제거 및 $PPAR{\alpha}$ 활성 실험결과에서 자유라디컬 제거능 및 항염증 효능이 높게 나타났다. 이러한 딱총나무 캘러스 세포 배양 추출물은 화장품의 항노화 신소재로서 응용될 수 있다.

• Korean Journal of Environment and Ecology
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• v.19 no.2
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• pp.90-98
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• 2005

To investigate the vegetation structure of the Woraksan National Park, twenty-two plots were set up and surveyed. According to the analysis of classification by TWINSP AN, the communities were divided by six groups; Pinus densiflora-Quercus serrata, Q. mongolica-Q. serrata, Q. mongolica-Betula davueica, P. densiflora-Q. mongolica, Q. mongolica, Q. mongolica-Acer pseudosieboldianum. Successional sere of the forest vegetation in the surveyed area were proeeding from Pinus densiflora to Quercus serrata, Quercus monogolica in the canopy layer group and from Rhododendron Rhododendron schlippenbachii to Fraxinus sieboldiana and Acer pseudosieboldianum in the understory layer group. But Q. mongolica might be edaphic climax species in some area.