Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

A Synergy Effect of Trisodium Phosphate and Ethanol on Inactivation of Murine Norovirus 1 on Lettuce and Bell Pepper

  • Kim, Eun-Jin (Department of Food Science and Biotechnology, Gachon University) ;
  • Lee, Young-Duck (Food Science and Engineering, School of Convergence Bioscience and Technology, Seowon University) ;
  • Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University Korea) ;
  • Park, Jong-Hyun (Department of Food Science and Biotechnology, Gachon University)
  • Received : 2015.03.11
  • Accepted : 2015.08.23
  • Published : 2015.12.28
Download PDF
⟨ Previous Next ⟩

Abstract

The synergy effect of trisodium phosphate (TSP) and ethanol against murine norovirus 1 (MNV-1), as a surrogate for human noroviruses, on fresh produces was evaluated. More than 2% (w/v) of TSP effectively inactivated MNV-1. The single treatment of 1% TSP or 30% ethanol for 30 min was not effective on MNV-1; however, cotreatment showed inactivation of MNV-1 on stainless steel and the produces of lettuce and bell pepper under 15 min. The results suggest that cotreatment of TSP and ethanol at a low concentration and a short time of exposure might be useful for the reduction of norovirus in some produce.

Keywords

References

  1. Behrsing J, Winkler S, Franz P, Premier R. 2000. Efficacy of chlorine for inactivation of Escherichia coli on vegetables. Postharvest Biol. Technol. 19: 187-192. https://doi.org/10.1016/S0925-5214(00)00092-2
  2. Belliot G, Lavaux A, Souihel D, Agnello D, Pothier P. 2008. Use of murine norovirus as a surrogate to evaluate resistance of human norovirus to disinfectants. Appl. Environ. Microbiol. 74: 3315-3318. https://doi.org/10.1128/AEM.02148-07
  3. Butot S, Putallaz T, Sanchez G. 2008. Effects of sanitation, freezing and frozen storage on enteric viruses in berries and herbs. Int. J. Food Microbiol. 126: 30-35. https://doi.org/10.1016/j.ijfoodmicro.2008.04.033
  4. Casteel MJ, Schmidt CE, Sobsey MD. 2008. Chlorine disinfection of produce to inactivate hepatitis A virus and coliphage MS2. Int. J. Food Microbiol. 125: 267-273. https://doi.org/10.1016/j.ijfoodmicro.2008.04.015
  5. Doultree JC, Druce JD, Birch CJ, Bowden DS, Marshall JA. 1999. Inactivation of feline calicivirus, a Norwalk virus surrogate. J. Hosp. Infect. 41: 51-57. https://doi.org/10.1016/S0195-6701(99)90037-3
  6. D'Souza DH, Su X. 2010. Efficacy of chemical treatments against murine norovirus, feline calicivirus, and MS2 bacteriophage. Foodborne Pathog. Dis. 7: 319-326. https://doi.org/10.1089/fpd.2009.0426
  7. Dubois E, Hennechart C, Merle G, Burger C, Hmila N, Ruelle S, Ferré V. 2007. Detection and quantification by real-time RT-PCR of hepatitis A virus from inoculated tap waters, salad vegetables, and soft fruits: characterization of the method performances. Int. J. Food Microbiol. 117: 141-149. https://doi.org/10.1016/j.ijfoodmicro.2007.02.026
  8. Fraisse A, Temmam S, Deboosere N, Guillier L, Delobel A, Maris P, Perelle S. 2011. Comparison of chlorine and peroxyacetic-based disinfectant to inactivate feline calicivirus, murine norovirus and hepatitis A virus on lettuce. Int. J. Food Microbiol. 151: 98-104. https://doi.org/10.1016/j.ijfoodmicro.2011.08.011
  9. Hsu CC, Riley LK, Livingston RS. 2007. Molecular characterization of three novel murine noroviruses. Virus Genes 34: 147-155. https://doi.org/10.1007/s11262-006-0060-1
  10. Koopmans M, Duizer E. 2004. Foodborne viruses: an emerging problem. Int. J. Food Microbiol. 90: 23-41. https://doi.org/10.1016/S0168-1605(03)00169-7
  11. Lages SLS, Ramakrishnan MA, Goyal SM. 2008. In-vivo efficacy of hand sanitisers against feline calicivirus: a surrogate for norovirus. J. Hosp. Infect. 68: 159-163. https://doi.org/10.1016/j.jhin.2007.11.018
  12. Le Guyader FS, Mittelholzer C, Haugarreau L, Hedlund KO, Alsterlund R, Pommepuy M, Svensson L. 2004. Detection of noroviruses in raspberries associated with a gastroenteritis outbreak. Int. J. Food Microbiol. 97: 179-186. https://doi.org/10.1016/j.ijfoodmicro.2004.04.018
  13. Li D, Baert L, De Jonghe M, Van Coillie E, Ryckeboer J, Devlieghere F, Uyttendaele M. 2011. Inactivation of murine norovirus 1, coliphage ϕX174, and Bacteroides fragilis phage B40-8 on surfaces and fresh-cut iceberg lettuce by hydrogen peroxide and UV light. Appl. Environ. Microbiol. 77: 2200-2205. https://doi.org/10.1128/AEM.00297-11
  14. Little CL, Gillespie IA. 2008. Prepared salads and public health. J. Appl. Microbiol. 105: 1729-1743. https://doi.org/10.1111/j.1365-2672.2008.03801.x
  15. Macinga DR, Sattar SA, Jaykus LA, Arbogast JW. 2008. Improved inactivation of nonenveloped enteric viruses and their surrogates by a novel alcohol-based hand sanitizer. Appl. Environ. Microbiol. 74: 5047-5052. https://doi.org/10.1128/AEM.00487-08
  16. McDonnell G, Russell AD. 1999. Antiseptics and disinfectants: activity, action, and resistance. Clin. Microbiol. Rev. 12: 147-179.
  17. Schmid D, Stüger HP, Lederer I, Pichler AM, Kainz-Arnfelser G, Schreier E, Allerberger F. 2007. A foodborne norovirus outbreak due to manually prepared salad, Austria 2006. Infection 35: 232-239. https://doi.org/10.1007/s15010-007-6327-1
  18. Su X, D'Souza DH. 2011. Trisodium phosphate for foodborne virus reduction on produce. Foodborne Pathog. Dis. 8: 713-717. https://doi.org/10.1089/fpd.2010.0763
  19. Tung G, Macinga D, Arbogast J, Jaykus LA. 2013. Efficacy of commonly used disinfectants for inactivation of human noroviruses and their surrogates. J. Food Protect. 76: 1210-1217. https://doi.org/10.4315/0362-028X.JFP-12-532
  20. Van der Poel WHM, Vinje J, van der Heide R, Herrera MI, Vivo A, Kloopmans MPG. 2000. Norwalk-like calicivirus genes in farm animals. Emerg. Infect. Dis. 6: 36-41. https://doi.org/10.3201/eid0601.000106
  21. Whitehead K, McCue KA. 2010. Virucidal efficacy of disinfectant actives against feline calicivirus, a surrogate for norovirus, in a short contact time. Am. J. Infect. Control 38: 26-30. https://doi.org/10.1016/j.ajic.2009.03.015

Cited by

  1. Pathogenesis of Human Norovirus Genogroup II Genotype 4 in Post-Weaning Gnotobiotic Pigs vol.28, pp.12, 2018, https://doi.org/10.4014/jmb.1809.09061