Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Fe(III)/Polydopamine-Mediated Capture and Release of Catecholic Compounds

  • Kim, Suyeob (Department of Marine Bio-Materials & Aquaculture, Pukyong National University) ;
  • Gim, Taewoo (Department of Marine Bio-Materials & Aquaculture, Pukyong National University) ;
  • Park, Ji Woo (Department of Marine Bio-Materials & Aquaculture, Pukyong National University) ;
  • Kim, Jongnam (Department of IT Convergence and Application Engineering, Pukyong National University) ;
  • Kang, Sung Min (Department of Marine Bio-Materials & Aquaculture, Pukyong National University)
  • Received : 2014.03.17
  • Accepted : 2014.05.12
  • Published : 2014.09.20
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Abstract

Keywords

Experimental

Materials. Dopamine hydrochloride (98%, Aldrich), trizma base (99%, Sigma), trizma HCl (99%, Sigma), iron(III) chloride hexahydrate (FeCl3·6H2O, 97%, Sigma-Aldrich), pyrocatechol violet (99%, Sigma-Aldrich), silica gel (99%, Sigma-Aldrich), and absolute ethanol (Merck) were used as received.

pDA Coating on Silica Beads. pDA coating was performed by combining silica gel (200-425 mesh particle size, 10 mg/mL) with a dopamine solution (10 mM, pH 8.5) at room temperature. After 1-hr coating, pDA-coated silica beads were separated from the suspension and washed several times by centrifuging/redispersing in water and ethanol.

Column Preparation and Fe(III) Complexation. 1 g of pDA-coated silica beads were suspended in ethanol and poured into the column. After packing, an ethanolic solution of FeCl3 (10 mM, 10 mL) was added to the column for the complexation reaction. Residual FeCl3 was then washed away with ethanol (50 mL).

Capture and Release of Pyrocatechol Violet (PCV). 8 μmol of PCV was dissolved in ethanol and passed through the column. Non-captured PCV was monitored by UV-Vis spectroscopy. Captured molecules were then released by eluting with an EDTA solution (1 mM, pH 3.5). Typically, the eluent was added onto a column and allowed to flow forced by gravity. The elution of the PCV was analyzed by UV-Vis spectroscopy, and the detection wavelength was 445 nm.

Characterizations. X-ray photoelectron spectra were obtained using a MultiLab 2000 system (Thermo VG Scientific, UK) with a Mg Kα X-ray source and ultrahigh vacuum (~10−10 mbar). UV-Vis spectroscopy was performed on a LAMBDA 35 UV/Vis spectrophotometer (Perkin Elmer).

References

  1. Waite, J. H.; Qin, X. Biochemistry 2001, 40, 2887. https://doi.org/10.1021/bi002718x
  2. Kroger, N.; Deutzmann, R.; Sumper, M. Science 1999, 286, 1129. https://doi.org/10.1126/science.286.5442.1129
  3. Miserez, A.; Schneberk, T.; Sun, C.; Zok, F. W.; Waite, J. H. Science 2008, 319, 1816. https://doi.org/10.1126/science.1154117
  4. Zhao, H.; Sun, C.; Stewart, R. J.; Waite, J. H. J. Biol. Chem. 2005, 280, 42938. https://doi.org/10.1074/jbc.M508457200
  5. Li, H.; Liu, Z. Trends Anal. Chem. 2012, 37, 148. https://doi.org/10.1016/j.trac.2012.03.010
  6. Holten-Andersen, N.; Fantner, G. E.; Hohlbauch, S.; Waite, J. H.; Zok, F. W. Nature Mater. 2007, 6, 669. https://doi.org/10.1038/nmat1956
  7. Holten-Andersen, N.; Mates, T. E.; Toprak, M. S.; Stucky, G. D.; Zok, F. W.; Waite, J. H. Langmuir 2008, 25, 3323.
  8. Holten-Andersen, N.; Zhao, H.; Waite, J. H. Biochemistry 2009, 48, 2752. https://doi.org/10.1021/bi900018m
  9. Harrington, M. J.; Masic, A.; Holten-Andersen, N.; Waite, J. H.; Fratzl, P. Science 2010, 328, 216. https://doi.org/10.1126/science.1181044
  10. Wilker, J. J. Angew. Chem. Int. Ed. 2010, 49, 8076. https://doi.org/10.1002/anie.201003171
  11. Zeng, H.; Hwang, D. S.; Israelachvili, J. N.; Waite, J. H. Proc. Natl. Aca. Sci. USA 2010, 107, 12850. https://doi.org/10.1073/pnas.1007416107
  12. Holten-Andersen, N.; Harrington, M. J.; Birkedal, H.; Lee, B. P.; Messersmith, P. B.; Lee, K. Y. C.; Waite, J. H. Proc. Natl. Aca. Sci. USA 2011, 108, 2651. https://doi.org/10.1073/pnas.1015862108
  13. Kim, S.; Kim, D. S.; Kang, S. M. Chem. Asian J. 2014, 9, 63. https://doi.org/10.1002/asia.201301291
  14. Lee, H.; Dellatore, S. M.; Miller, W. M.; Messersmith, P. B. Science 2007, 318, 426. https://doi.org/10.1126/science.1147241
  15. Kang, S. M.; You, I.; Cho, W. K.; Shon, H. K.; Lee, T. G.; Choi, I. S.; Karp, J. M.; Lee, H. Angew. Chem. Int. Ed. 2010, 49, 9401. https://doi.org/10.1002/anie.201004693
  16. Ryu, J.; Ku, S. H.; Lee, H.; Park, C. B. Adv. Func. Mater. 2010, 20, 2132. https://doi.org/10.1002/adfm.200902347
  17. Hong, S.; Na, Y. S.; Choi, S.; Song, I. T.; Kim, W. Y.; Lee, H. Adv. Func. Mater. 2012, 22, 4711. https://doi.org/10.1002/adfm.201201156