농약과학회지 (The Korean Journal of Pesticide Science)

  • 제18권2호
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  • Pages.104-114
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  • 2014
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  • 1226-6183(pISSN)
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  • 2287-2051(eISSN)
한국농약과학회 (The Korean Society of Pesticide Science)
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사군자추출물의 깍지벌레류에 대한 살충활성

Insecticidal Activity of Chinese Honeysuckle, Quisqualis indica Extracts against Scale Insects

  • 송진선 (원예특작과학원 원예특작환경과) ;
  • 이채민 (국립산림과학원 산림병해충과) ;
  • 최용화 (경북대학교 생태환경대학 생태환경시스템학부) ;
  • 이동운 (경북대학교 생태환경대학 생태환경관광학부)
  • Song, Jin Sun (Horticultural & Herbal Crop Environment Division, National Horticulture of Horticultural & Herbal Science) ;
  • Lee, Chae Min (Division of Forest Insect Pests and Diseases, Korea Forest Research Institute) ;
  • Choi, Young Hwa (School of Ecology and Environmental System, College of Ecology and Environment Sciences, Kyungpook National University) ;
  • Lee, Dong Woon (School of Ecology, Environmente and Tourism, College of Ecology and Environment Sciences, Kyungpook National University)
  • 투고 : 2014.04.01
  • 심사 : 2014.05.20
  • 발행 : 2014.06.30
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초록

깍지벌레는 농작물과 도심 및 산림의 수목에 심각한 피해를 주는 해충이다. 본 연구는 이러한 깍지벌레류의 환경친화적 대체방제법을 강구하기 위하여 수행하였는데 기존의 연구에서 솔껍질깍지벌레(Matsucoccus thunbergianae)에 대하여 온수추출물이 효과가 있었던 사군자(Quisqualis indica)를 이용하여 수행하였다. 사군자 열매 메탄올 추출물을 헥산과 클로르포름, 에칠아세테이트, 부탄올, 물층으로 분획하여 귤가루깍지벌레(Planococcus citri)와 주머니깍지벌레(Eriococcus lagerstroemiae), 거북밀깍지벌레(Ceroplastes japonicus), 소나무가루깍지벌레(Crisicoccus pini) 약충을 대상으로 살충활성을 검정한 결과 헥산층의 살충 활성이 가장 높았다. 헥산층에서 활성물질을 탐색한 결과 alpha-pinene, methyl palmitate, eugenol, methyl myristate, phthalic acid mono (2-ethylhexyl)ester와 palmitic acid가 확인 되었으며 그들 물질들 중 ${\alpha}$-pinene의 주머니깍지벌레 2령충에 대한 반수치사농도($LC_{50}$)는 0.0114 ppm이었다. 사군자 추출물은 깍지벌레류의 대체 방제제로 활용할 수 있을 것으로 생각된다.

Scale insects heavily damaged many agricultural crops and trees in urban and forest areas. This study was conducted to carry out alternative control methods for environmentally friendly control of scale insects. Hot water extract of Quisquali indica produced high mortality against the black pine bast scale, Matsucoccus thunbergianae in previously experiment. Methanol extract obtained from fruit of Q. indica was successively fractionated using hexane, chloroform, ethyl acetate, buthanol and water which were examined by exposing for their insecticidal activity against four Cocoidea species (Eriococcus lagerstroemiae, Ceroplastes japonicus, Crisicoccus pini and Planococcus citri) in laboratory. Hexane fraction gave the highest insecticidal activity against scale insect pest. GC-mass analysis confirmed that the main compounds of Q. indica were alpha-pinene, methyl palmitate, eugenol, methyl myristate, phthalic acid mono(2-ethylhexyl)ester and palmitic acid. Among those compounds, ${\alpha}$-pinene was included 10 hexane subfractions. $LC_{50}$ of ${\alpha}$-pinene was 0.0114 ppm against 2nd instar of E. lagerstroemiae. These results suggest that Q. indica extracts might be used as an alternative control agent of scale insects.

키워드

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피인용 문헌

  1. Control Efficacy of Eclipta prostrata Extract against Cyst Nematode, Heterodera spp. vol.23, pp.1, 2019, https://doi.org/10.7585/kjps.2018.23.1.26