The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Gami-Chunggisan on Antioxidant and Pro-Inflammatory Cytokine

가미청기산(加味淸肌散)이 항산화와 염증성 사이토카인에 미치는 영향

  • Lee, Youn-Jeong (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Sim, Boo-Yong (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Lee, Hae-Jin (Department college of Beauty & Healthy Medicine, Daejeon University) ;
  • Bak, Ji-Won (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • 이윤정 (대전대학교 한의학대학 병리학교실) ;
  • 심부용 (대전대학교 한의학대학 병리학교실) ;
  • 이해진 (대전대학교 미용의학) ;
  • 박지원 (대전대학교 한의학대학 병리학교실) ;
  • 김동희 (대전대학교 한의학대학 병리학교실)
  • Received : 2014.06.29
  • Accepted : 2014.07.23
  • Published : 2014.07.30
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Abstract

Objectives : Gami-Chunggisan extract (GCE) is one of the oriental traditional medicine. We investigated the antioxidant effect and reduction of pro-inflammatory cytokine as a functional ingredient for cosmetic products from the GCE. Methods : GCE was prepared by extracting with 80% ethanol. We analyzed total polyphenol and antioxidant activities. To evaluate antioxidant activity, we measured 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. Also we measured the production of reactive oxygen species (ROS) and nitric oxide (NO) on Raw264.7 cells. We researched reduction of anti-inflammatory cytokines from concentration of GCE on Raw264.7 cells. Results : Total polyphenol quantity of GCE was included 46.6 mg/g. The GCE showed ABTS free radical scavenging ability with more than 89% at $1000{\mu}g/m{\ell}$. In addition the DPPH free radical scavenging ability from the GCE was activated over 93% at $1000{\mu}g/m{\ell}$. Production of the ROS was decreased by approximately 26%, upon the GCE treatment at concentration of $100{\mu}g/m{\ell}$. The GCE at $100{\mu}g/m{\ell}$ concentration showed inhibitory effect on NO production by 38%. Production of IL-$1{\beta}$ and IL-6 were decreased by approximately 56% and 36%, respectively upon GCE treatment at $100{\mu}g/m{\ell}$. Also, production of TNF-${\alpha}$ was decreased by approximately 79% at $100{\mu}g/m{\ell}$. Moreover, the GCE showed inhibitory effects on the expression of the IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ genes in LPS-induced Raw 264.7 cells. Conclusions : From the results above, we conclude that the GCE indicated significant antioxidant effects and induced reduction of pro-inflammatory cytokine.

Keywords

References

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