The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory Effect of Houttuyniae Herba Water Extract on LPS-induced RAW 264.7 Mouse Macrophages

마우스 대식세포를 이용한 어성초(魚腥草) 물추출물의 항염효능연구

  • Hwang, In Seung (Department of Pathology, College of Korean Medicine, Gachon University) ;
  • Kim, Young Jin (Department of Pathology, College of Korean Medicine, Gachon University) ;
  • Park, Yun Soo (Department of Pathology, College of Korean Medicine, Gachon University) ;
  • Kim, Hyun Ju (Department of Pathology, College of Korean Medicine, Gachon University) ;
  • Kim, Do Hoon (Department of Oriental Medical Classics & History, College of Korean Medicine, Gachon University) ;
  • Park, Wan Su (Department of Pathology, College of Korean Medicine, Gachon University)
  • 황인승 (가천대학교 한의과대학 병리학교실) ;
  • 김영진 (가천대학교 한의과대학 병리학교실) ;
  • 박윤수 (가천대학교 한의과대학 병리학교실) ;
  • 김현주 (가천대학교 한의과대학 병리학교실) ;
  • 김도훈 (가천대학교 한의과대학 원전의사학교실) ;
  • 박완수 (가천대학교 한의과대학 병리학교실)
  • Received : 2014.06.15
  • Accepted : 2014.07.17
  • Published : 2014.07.30
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Abstract

Objectives : The purpose of this study was to investigate effects of Houttuyniae Herba water extract (HC) on calcium release and production of various inflammatory mediators such as nitric oxide (NO), interferon-inducible protein (IP)-10, platelet derived growth factor (PDGF)-BB, keratinocyte-derived chemokine (KC), vascular endothelial growth factor (VEGF), interleukin (IL)-4, and IL-5 in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages. Methods : NO production was measured by Griess reagent assay. Intracellular calcium level was measured with Fluo-4 assay. Levels of cytokines were measured by High-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology. Results : HC significantly decreased NO production for 24 hrs incubation at the concentrations of 10, 25, 50, 100, and $200{\mu}/mL$ in LPS-induced RAW 264.7 (P < 0.05). HC significantly decreased production of IP-10, KC, VEGF, and PDGF-BB for 24 hrs incubation at the concentrations of 50, 100, and $200{\mu}/mL$ in LPS-induced RAW 264.7 (P < 0.05). HC also significantly decreased intracellular calcium release for 24 hrs incubation at the concentrations of 25, 50, 100, and $200{\mu}/mL$ in LPS-induced RAW 264.7 (P < 0.05). But HC did not show any significant effect on production of IL-4 and IL-5 in LPS-induced RAW 264.7. Conclusions : The results suggested that HC has anti-inflammatory property related with its inhibition on the production of NO, IP-10, KC, VEGF, and PDGF-BB in LPS-induced macrophages via calcium pathway.

Keywords

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