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http://dx.doi.org/10.6116/kjh.2014.29.4.69.

Effect of Gami-Chunggisan on Antioxidant and Pro-Inflammatory Cytokine  

Lee, Youn-Jeong (Department of Pathology, College of Oriental Medicine, Daejeon University)
Sim, Boo-Yong (Department of Pathology, College of Oriental Medicine, Daejeon University)
Lee, Hae-Jin (Department college of Beauty & Healthy Medicine, Daejeon University)
Bak, Ji-Won (Department of Pathology, College of Oriental Medicine, Daejeon University)
Kim, Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
Publication Information
The Korea Journal of Herbology / v.29, no.4, 2014 , pp. 69-76 More about this Journal
Abstract
Objectives : Gami-Chunggisan extract (GCE) is one of the oriental traditional medicine. We investigated the antioxidant effect and reduction of pro-inflammatory cytokine as a functional ingredient for cosmetic products from the GCE. Methods : GCE was prepared by extracting with 80% ethanol. We analyzed total polyphenol and antioxidant activities. To evaluate antioxidant activity, we measured 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging assay. Also we measured the production of reactive oxygen species (ROS) and nitric oxide (NO) on Raw264.7 cells. We researched reduction of anti-inflammatory cytokines from concentration of GCE on Raw264.7 cells. Results : Total polyphenol quantity of GCE was included 46.6 mg/g. The GCE showed ABTS free radical scavenging ability with more than 89% at $1000{\mu}g/m{\ell}$. In addition the DPPH free radical scavenging ability from the GCE was activated over 93% at $1000{\mu}g/m{\ell}$. Production of the ROS was decreased by approximately 26%, upon the GCE treatment at concentration of $100{\mu}g/m{\ell}$. The GCE at $100{\mu}g/m{\ell}$ concentration showed inhibitory effect on NO production by 38%. Production of IL-$1{\beta}$ and IL-6 were decreased by approximately 56% and 36%, respectively upon GCE treatment at $100{\mu}g/m{\ell}$. Also, production of TNF-${\alpha}$ was decreased by approximately 79% at $100{\mu}g/m{\ell}$. Moreover, the GCE showed inhibitory effects on the expression of the IL-$1{\beta}$, IL-6 and TNF-${\alpha}$ genes in LPS-induced Raw 264.7 cells. Conclusions : From the results above, we conclude that the GCE indicated significant antioxidant effects and induced reduction of pro-inflammatory cytokine.
Keywords
Gami-Chunggisan; antioxidant; free radical; anti-inflammation; cytokine;
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