Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana

  • Li, Wei (School of Biotechnology, Yeungnam University) ;
  • Yan, Xi Tao (College of Pharmacy, Chungnam National University) ;
  • Sun, Ya Nan (College of Pharmacy, Chungnam National University) ;
  • Ngan, Thi Thanh (College of Pharmacy, Chungnam National University) ;
  • Shim, Sang Hee (School of Biotechnology, Yeungnam University) ;
  • Kim, Young Ho (College of Pharmacy, Chungnam National University)
  • Received : 2014.04.23
  • Accepted : 2014.06.02
  • Published : 2014.07.31
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Abstract

In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-${\kappa}B$ inhibitory activity of the isolated compounds was measured in $TNF{\alpha}$-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited $TNF{\alpha}$-stimulated NF-${\kappa}B$ activation in a dose-dependent manner, with $IC_{50}$ values ranging from $0.75-8.30{\mu}M$. Compounds 19 and 20 also inhibited the $TNF{\alpha}$-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with $EC_{50}$ values ranging from $0.9-10.8{\mu}M$. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.

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References

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