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http://dx.doi.org/10.4062/biomolther.2014.047

Anti-Inflammatory and PPAR Transactivational Effects of Oleanane-Type Triterpenoid Saponins from the Roots of Pulsatilla koreana  

Li, Wei (School of Biotechnology, Yeungnam University)
Yan, Xi Tao (College of Pharmacy, Chungnam National University)
Sun, Ya Nan (College of Pharmacy, Chungnam National University)
Ngan, Thi Thanh (College of Pharmacy, Chungnam National University)
Shim, Sang Hee (School of Biotechnology, Yeungnam University)
Kim, Young Ho (College of Pharmacy, Chungnam National University)
Publication Information
Biomolecules & Therapeutics / v.22, no.4, 2014 , pp. 334-340 More about this Journal
Abstract
In this study, 23 oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. The NF-${\kappa}B$ inhibitory activity of the isolated compounds was measured in $TNF{\alpha}$-treated HepG2 cells using a luciferase reporter system. Compounds 19-23 inhibited $TNF{\alpha}$-stimulated NF-${\kappa}B$ activation in a dose-dependent manner, with $IC_{50}$ values ranging from $0.75-8.30{\mu}M$. Compounds 19 and 20 also inhibited the $TNF{\alpha}$-induced expression of iNOS and ICAM-1 mRNA. Moreover, effect of the isolated compounds on PPARs transcriptional activity was assessed. Compounds 7-11 and 19-23 activated PPARs the transcriptional activity significantly in a dose-dependent manner, with $EC_{50}$ values ranging from $0.9-10.8{\mu}M$. These results suggest the presence of potent anti-inflammatory components in P. koreana, and will facilitate the development of novel anti-inflammatory agents.
Keywords
Pulsatilla koreana; NF-${\kappa}B$ inhibitory activity; Tumor necrosis factor-${\alpha}$; PPAR;
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