Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enzymatic Production of 15-Hydroxyeicosatetraenoic Acid from Arachidonic Acid by Using Soybean Lipoxygenase

  • Kim, Baek-Joong (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Shin, Kyung-Chul (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Oh, Deok-Kun (Department of Bioscience and Biotechnology, Konkuk University)
  • Received : 2013.10.15
  • Accepted : 2013.11.29
  • Published : 2014.03.28
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Abstract

15-Hydroxyeicosatetraenoic acid (HETE), as a mammalian biologically active metabolite, has anticarcinogenic effect. The conditions of producing 15-HETE from arachidonic acid by using soybean lipoxygenase were optimal at pH 8.5 and $20^{\circ}C$ with 9 g/l arachidonic acid, 54.4 U/ml soybean lipoxygenase, and 4% methanol. Under these optimized conditions, the enzyme produced 9.5 g/l 15-HETE after 25 min, with a molar conversion yield of 99% and a productivity of $22.8gl^{-1}h^{-1}$. To the best of our knowledge, this is the first biotechnological production of 15-HETE.

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References

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