한국균학회지 (The Korean Journal of Mycology)

  • 제42권1호
  • /
  • Pages.64-68
  • /
  • 2014
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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해외도입 표고버섯의 진균바이러스 감염

Infection of Mycovirus in Imported Lentinula edodes

  • 이송희 (경상대학교 미생물학과) ;
  • 곽서영 (경상대학교 미생물학과) ;
  • 고한규 (산림조합중앙회 산림버섯연구소) ;
  • 이현숙 (경상대학교 미생물학과)
  • Lee, Song Hee (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University) ;
  • Kwak, Seo-Young (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University) ;
  • Ko, Han Kyu (Forest Mushroom Research Institute) ;
  • Lee, Hyun-Sook (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University)
  • 투고 : 2014.03.06
  • 심사 : 2014.03.21
  • 발행 : 2014.03.31
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초록

최근 몇몇 버섯의 바이러스가 보고되었으며, Lentinula edodes Spherical Virus (LeSV) 역시 보고되었다. 곰팡이 바이러스는 종균에 의해 전파되기 때문에 농장에서 버섯 바이러스병을 근절하기 위해서는 건전 종균을 사용하는 것이 중요하다. 이에 표고버섯 바이러스인 LeSV의 RdRp 유전자에 특이적인 primer 및 RT-PCR 조건을 이용하여 해외에서 도입한 표고종균을 대상으로 LeSV 감염도를 조사하였다. 분석결과 해외도입 종균의 99%가 LeSV에 감염된 것으로 나타났다.

Up to date several mycoviruses including Lentinula edodes Spherical Virus (LeSV) have been reported. As fungal virus was spreaded by infested hypae and spores it could be important to use virus-free spawns to eradicate the mushroom virus disease in the culture farm. We tested the imported spawns of Lentinula edodes by PCR whether LeSV was infested them or not. The primer set targeting the RdRp gene of LeSV was prepared based on partial sequence of the LeSV genome. The RT-PCR analysis showed that 87 among 88 imported spawns of L. edodes were infested by LeSV.

키워드

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