한국균학회지 (The Korean Journal of Mycology)

  • 제42권1호
  • /
  • Pages.69-73
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  • 2014
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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세면대 실리콘에 발생한 곰팡이의 동정

Identification of Fungi Occurred on Silicon of Washbasin

  • 홍승범 (국립농업과학원 농업미생물과) ;
  • 김대호 (국립농업과학원 농업미생물과)
  • Hong, Seung-Beom (Korean Agricultural Culture Collection, Agricultural Microbiology Div. National Academy of Agricultural Science, RDA) ;
  • Kim, Dae-Ho (Korean Agricultural Culture Collection, Agricultural Microbiology Div. National Academy of Agricultural Science, RDA)
  • 투고 : 2014.03.15
  • 심사 : 2014.03.17
  • 발행 : 2014.03.31
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초록

아파트 욕실 세면대의 실리콘에 검은색의 곰팡이가 발생하였다. 곰팡이가 발생한 부위의 실리콘을 MEA, DG18, DRBC, PDA+PS 배지에 치상하여 18균주의 곰팡이를 분리하고, 형태적 그리고 분자생물학적인 방법으로 동정하였다. 이들은 Phoma herbarum, Peyronellaea calorpreferens, Cladosporim halotolerans로 동정되었으며 이 중에서 Ph. herbarum, Pe. calorpreferens는 높은 빈도로 분리되었으나 C. halotolerans는 분리 빈도가 높지 않았다. 이들은 농업미생물은행(KACC)에 장기 보존되었으며 앞으로 관련 연구에 제공된다.

Black molds were occurred on silicon of washbasin in a bathroom in apartment. Blocks of silicon where the fungi grew, were placed on MEA, DG18, DRBC, PDA+PS media, and 18 strains of fungi were isolated. They were identified as Phoma herbarum, Peyronellaea calorpreferens, Cladosporim halotolerans based on morphological and molecular methods. Ph. herbarum and Pe. calorpreferens were isolated with high frequency, but C. halotolerans was isolated with low frequency. The fungal strains were deposited in Korean Agricultural Culture Collection (KACC), and they shall be used for further related researches.

키워드

참고문헌

  1. Moriyama Y, Nawata N, Tsuda T, Nitta M. Occurrence of moulds in Japanese bathrooms. Int Biodeter Biodegr 1992;30: 47-55. https://doi.org/10.1016/0964-8305(92)90023-H
  2. Hamada N. Effect of materials on mold contamination in bathrooms. J Antibact Antifung Agents 2008;12:829-835.
  3. Ihara N, Ishiki S, Hamada N. Effect of hypochlorite in the fungal contamination in the silicone in bathrooms. J Antibact Antifung Agents 2009;37:91-97.
  4. Hong SB, Kim DH, Lee M, Baek SY, Kwon SW, Samson RA. Taxonomy of Eurotium species isolated from Meju. J Microbiol 2011;49:669-674. https://doi.org/10.1007/s12275-011-0376-y
  5. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. App Environ Microbiol 1995;61:1323-1330.
  6. Bensch K, Groenewald JZ, Dijksterhuis J, Starink-Willemse M, Andersen B, Summerell BA, Shin HD, Dugan FM, Schroers HJ, Braun U, Crous PW. Species and ecological diversity within the Cladosporium cladosporioides complex (Davidiellaceae, Capnodiales). Stud Mycol 2010;67:1-94. https://doi.org/10.3114/sim.2010.67.01
  7. Aveskamp MM, Gruyter J, Murace MA, Perello A, Woudenberg JHC, Groenewald JZ, Crous PW. DNA phylogeny reveals polyphyly of Phoma section Peyronellaea and multiple taxonomic novelties. Mycologia 2009;101:363-382. https://doi.org/10.3852/08-199
  8. Bensch K, Braun U, Groenewald JG, Crous PW. The genus Cladosporium. Stud Mycol 2012;72:1-401. https://doi.org/10.3114/sim0003
  9. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol Biol Evol 2011;28:2731-2739. https://doi.org/10.1093/molbev/msr121
  10. Web site of Alaska Department of Fih and Game [Internet]. [Cited 24 Feb. 2014]. Available from: http://www.adfg.alaska. gov/static/species/disease/pdfs/fishdiseases/phoma_herbarum.pdf
  11. Selbmann L, Onofri S, Fenice M, Federici F, Petruccioli M. Production and structural characterization of the exopolysaccharide of the Antarctic fungus Phoma herbarum CCFEE 5080. Research Microbiol 2002;153:585-592. https://doi.org/10.1016/S0923-2508(02)01372-4
  12. Boerema GH, Gruyter J, Noordeloos ME, Hamers MEC. Phoma identification manual. Differentiation of specific and infra-specific taxa in culture. CABI publishing; Wallingford, U.K.; 2004.
  13. Aveskamp MM, Gruyter J, Woudenberg JHC, Verkley GJM, Crous PW. Highlights of the Didymellaceae: A polyphasic approach to characterize Phoma and related pleosporalean genera. Stud Mycol 2010;65:1-60. https://doi.org/10.3114/sim.2010.65.01