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http://dx.doi.org/10.4489/KJM.2014.42.1.64

Infection of Mycovirus in Imported Lentinula edodes  

Lee, Song Hee (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University)
Kwak, Seo-Young (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University)
Ko, Han Kyu (Forest Mushroom Research Institute)
Lee, Hyun-Sook (Department of Microbiology and Research Institute for Life Science, Gyeongsang National University)
Publication Information
The Korean Journal of Mycology / v.42, no.1, 2014 , pp. 64-68 More about this Journal
Abstract
Up to date several mycoviruses including Lentinula edodes Spherical Virus (LeSV) have been reported. As fungal virus was spreaded by infested hypae and spores it could be important to use virus-free spawns to eradicate the mushroom virus disease in the culture farm. We tested the imported spawns of Lentinula edodes by PCR whether LeSV was infested them or not. The primer set targeting the RdRp gene of LeSV was prepared based on partial sequence of the LeSV genome. The RT-PCR analysis showed that 87 among 88 imported spawns of L. edodes were infested by LeSV.
Keywords
Detection; Lentinula edodes; Mycovirus; RT-PCR;
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