Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a

  • Song, JaeHyoung (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Yeo, Sang-Gu (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Hong, Eun-Hye (College of Pharmacy, Kangwon National University) ;
  • Lee, Bo-Ra (College of Pharmacy, Kangwon National University) ;
  • Kim, Jin-Won (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Kim, JeongHoon (Ahn-Gook Pharmaceutical Co., LTD.) ;
  • Jeong, HyeonGun (Ahn-Gook Pharmaceutical Co., LTD.) ;
  • Kwon, YongSoo (College of Pharmacy, Kangwon National University) ;
  • Kim, HyunPyo (College of Pharmacy, Kangwon National University) ;
  • Lee, SangWon (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention) ;
  • Park, Jae-Hak (Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Ko, Hyun-Jeong (College of Pharmacy, Kangwon National University)
  • Received : 2013.12.20
  • Accepted : 2014.01.15
  • Published : 2014.01.31
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Abstract

Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that hederasaponin B and 30% ethanol extract of Hedera helix containing hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that hederasaponin B and Hedera helix extract containing hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71.

Keywords

References

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