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http://dx.doi.org/10.4062/biomolther.2013.108

Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a  

Song, JaeHyoung (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention)
Yeo, Sang-Gu (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention)
Hong, Eun-Hye (College of Pharmacy, Kangwon National University)
Lee, Bo-Ra (College of Pharmacy, Kangwon National University)
Kim, Jin-Won (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention)
Kim, JeongHoon (Ahn-Gook Pharmaceutical Co., LTD.)
Jeong, HyeonGun (Ahn-Gook Pharmaceutical Co., LTD.)
Kwon, YongSoo (College of Pharmacy, Kangwon National University)
Kim, HyunPyo (College of Pharmacy, Kangwon National University)
Lee, SangWon (Division of Vaccine Research, Center for Infectious Diseases, National Institute of Health, Korea Centers for Diseases Control and Prevention)
Park, Jae-Hak (Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University)
Ko, Hyun-Jeong (College of Pharmacy, Kangwon National University)
Publication Information
Biomolecules & Therapeutics / v.22, no.1, 2014 , pp. 41-46 More about this Journal
Abstract
Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that hederasaponin B and 30% ethanol extract of Hedera helix containing hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that hederasaponin B and Hedera helix extract containing hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71.
Keywords
Enterovirus 71; Antiviral activity; Hederasaponin B; Hedera helix; Hand foot and mouth disease;
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