Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidative activities of Artemisia capillaris-Fermented Hericium erinaceum Mycelium

인진쑥 노루궁뎅이 버섯균사체 발효물의 항산화 활성

  • 김승섭 (청주대학교 바이오메디컬학과) ;
  • 경인구 ((주)코시스바이오 기업부설연구소) ;
  • 이미라 ((주)코시스바이오 기업부설연구소) ;
  • 김동구 ((주)코시스바이오 기업부설연구소) ;
  • 신지영 ((주)코시스바이오 기업부설연구소) ;
  • 양진이 (청주대학교 바이오메디컬학과) ;
  • 이광호 (청주대학교 바이오메디컬학과) ;
  • 음원식 (한림대학교 바이오메디컬학과) ;
  • 강정훈 (청주대학교 바이오메디컬학과)
  • Received : 2014.11.20
  • Accepted : 2014.12.16
  • Published : 2014.12.30
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Abstract

The hot water extract from Artemisia capillaris fermented with Hericium erinaceum mycelium (AC-HE) were assessed for the protection against oxidative modification of biological macromolecules and cell death. Antioxidant activity of AC-HE evaluated using 2,2-diphenyl-1-picrylhydrazyl radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical and peroxyl radical scavenging assays. AC-HE showed 61.73% DPPH radical scavenging activity at $500{\mu}g/mL$, 97.39% ABTS radical scavenging activity at $250{\mu}g/mL$, and 44.18% peroxyl radical scavenging activity at $100{\mu}g/mL$. AC-HE were shown to significantly inhibited DNA strand breakage induced by peroxyl radical. AC-HE also prevented peroxyl radical-mediated human serum albumin modification. AC-HE effectively inhibited $H_2O_2$ induced cell death and significantly increased of the 11.47% cell survival at $100{\mu}g/mL$. AC-HE also decreased intracellular reactive oxygen species (ROS) levels in $H_2O_2$-treated cells. The results suggested that AC-HE can contribute to antioxidant and protected cells from oxidative stress-induced cell injury.

노루궁뎅이 버섯균사체(Hericium erinaceum)를 인진쑥(Artemisia capillaris)에 배양하여 조제한 AC-HE 열수 추출물이 생체고분자의 산화적 손상과 세포사멸을 보호할 수 있는 지를 관찰하였다. AC-HE의 항산화 활성을 DPPH radical, ABTS radical, peroxyl radical 소거활성 측정을 통해 알아보았다. 그 결과 DPPH radical 소거활성은 $500{\mu}g/mL$ 농도에서 61.73%, ABTS radical 소거활성은 $250{\mu}g/mL$ 농도에서 97.39%, peroxyl radical 소거활성은 $100{\mu}g/mL$ 농도에서 44.18%로 나타났다. AC-HE은 DNA의 산화적 손상을 효과적으로 억제하였다. 또한 생체고분자물질인 사람의 혈청단백질의 산화적 손상을 억제하였다. 세포에 $H_2O_2$를 처리하였을 때 세포생존율에 비하여 발효물을 $100{\mu}g/mL$ 농도로 전 처리한 세포생존율은 11.47% 증가했으며, 발효물을 $50{\mu}g/mL$ 농도로 처리했을 경우 세포내 ROS의 축적이 유의적으로 감소되었다. 따라서 AC-HE은 항산화 활성뿐만 아니라 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용이 뛰어난 것으로 사료되었다.

Keywords

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