Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Comparative Radical Scavenging and Nitric Oxide Production Capacity of Water Extracts of Acanthopanax divaricatus var, Albeofructus and Eleutherococcus divaricatus var. Chiisanensis

지리산오가피와 흰털오가피 열수추출물의 라디칼 제거 활성 및 대식세포에서의 산화질소 생성능 비교

  • Kim, Dong-Min (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Jin, Bora (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Kim, Ji Yeon (Department of Food Science and Technology, Seoul National University of Science and Technology)
  • Received : 2014.04.09
  • Accepted : 2014.07.10
  • Published : 2014.12.31
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Abstract

In the present study, water extracts of Acanthopanax divaricatus var, albeofructus and Eleutherococcus divaricatus var. chiisanensis were prepared and their antioxidant and anti-inflammatory effects were compared. The water extract of Acanthopanax divaricatus var, albeofructus showed significantly higher activity in total polyphenol and flavonoid contents, while there were no significant differences in ABTS/DPPH radical scavenging activities and ferric reducing antioxidant power. However, the water extract of Eleutherococcus divaricatus var. chiisanensis at the highest dose showed significantly lower capacity in nitric oxide production than that of Acanthopanax divaricatus var, albeofructus. Taken together, there were differences between Acanthopanax divaricatus var, albeofructus and Eleutherococcus divaricatus var. chiisanensis in total polyphenol/flavonoid contents and anti-inflammatory effect, however, the antioxidant effect was almost similar.

본 연구에서는 지리산오가피와 흰털오가피의 항산화 및 항염증 활성의 차이를 알아보기 위해 총 폴리페놀 및 플라보노이드 함량, ABTS 및 DPPH 라디칼 소거능, FRAP assay를 이용한 환원력을 비교 조사하였고, mouse의 대식세포인 Raw 264.7 cell에 LPS를 이용하여 자극시킨 후 생성되는 NO의 함량을 비교하였다. 총 폴리페놀 및 플라보노이드 함량은 흰털오가피가 유의하게 높은 것으로 나타났으나 그 차이가 크지 않으며 ABTS 및 DPPH 라디칼 소거능과 FRAP 방법을 이용한 환원력에서는 두 오가피 종에서 차이가 없는 것으로 나타났다. 항염증 활성을 비교하기 위해 확인한 NO 생성양은 지리산오가피가 고농도 처리시 유의하게 항염증 활성이 높은 것으로 나타났다.

Keywords

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