Journal of Microbiology and Biotechnology

  • 제23권4호
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  • Pages.444-450
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  • 2013
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Novosphingobium ginsenosidimutans sp. nov., with the Ability to Convert Ginsenoside

  • Kim, Jin-Kwang (KI for the BioCentry, Korea Advanced Institute of Science and Technology) ;
  • He, Dan (KI for the BioCentry, Korea Advanced Institute of Science and Technology) ;
  • Liu, Qing-Mei (KI for the BioCentry, Korea Advanced Institute of Science and Technology) ;
  • Park, Hye-Yoon (Microorganism Resources Division, National Institute of Biological Resources) ;
  • Jung, Mi-Sun (College of Humanities and Social Science, Youngdong University) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Sun-Chang (KI for the BioCentry, Korea Advanced Institute of Science and Technology) ;
  • Im, Wan-Taek (KI for the BioCentry, Korea Advanced Institute of Science and Technology)
  • 투고 : 2012.12.26
  • 심사 : 2013.01.31
  • 발행 : 2013.04.28
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초록

A Gram-negative, strictly aerobic, non-motile, non-spore-forming, and rod-shaped bacterial strain designated FW-$6^T$ was isolated from a freshwater sample and its taxonomic position was investigated by using a polyphasic approach. Strain FW-$6^T$ grew optimally at $10-42^{\circ}C$ and at pH 7.0 on nutrient and R2A agar. Strain FW-$6^T$ displayed ${\beta}$-glucosidase activity that was responsible for its ability to transform ginsenoside $Rb_1$ (one of the dominant active components of ginseng) to Rd. On the basis of 16S rRNA gene sequence similarity, strain FW-$6^T$ was shown to belong to the family Sphingomonadaceae and was related to Novosphingobium aromaticivorans DSM $12444^T$ (98.1% sequence similarity) and N. subterraneum IFO $16086^T$ (98.0%). The G+C content of the genomic DNA was 64.4%. The major menaquinone was Q-10 and the major fatty acids were summed feature 7 (comprising $C_{18:1}{\omega}9c/{\omega}12t/{\omega}7c$), summed feature 4 (comprising $C_{16:1}{\omega}7c/iso-C_{15:0}2OH$), $C_{16:0}$, and $C_{14:0}$ 2OH. DNA and chemotaxonomic data supported the affiliation of strain FW-$6^T$ to the genus Novosphingobium. Strain FW-$6^T$ could be differentiated genotypically and phenotypically from the recognized species of the genus Novosphingobium. The isolate that has ginsenoside converting ability therefore represents a novel species, for which the name Novosphingobium ginsenosidimutans sp. nov. is proposed, with the type strain FW-$6^T$ (= KACC $16615^T$ = JCM $18202^T$).

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