한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제28권2호
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  • Pages.133-139
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  • 2013
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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Post-Activation Treatment with Cytochalasins and Latrunculin A on the Development of Pig Oocytes after Parthenogenesis and Somatic Cell Nuclear Transfer

  • Park, Bola (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Joohyeong (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) ;
  • Lee, Yongjin (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) ;
  • Elahi, Fazle (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University) ;
  • Jeon, Yubyeol (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Hyun, Sang-Hwan (Laboratory of Veterinary Embryology and Biotechnology, College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Eunsong (Labortory of Theriogenology, College of Veterinary Medicine, Kangwon National University)
  • 투고 : 2013.04.30
  • 심사 : 2013.05.25
  • 발행 : 2013.06.30
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초록

The objective of this study was to determine the effect of post-activation treatment with cytoskeletal regulators in combination with or without 6-dimethylaminopurine (DMAP) on embryonic development of pig oocytes after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT). PA and SCNT oocytes were produced by using in vitro-matured pig oocytes and treated for 4 h after electric activation with $0.5{\mu}M$ latrunculin A (LA), $10.4{\mu}M$ cytochalasins B (CB), and $4.9{\mu}M$ cytochalasins D (CD) together with none or 2 mM DMAP. Post-activation treatment of PA oocytes with LA, CB, and CD did not alter embryo cleavage (85.8~88.6%), blastocyst formation (30.7~ 32.4%), and mean cell number of blastocysts (33.5~33.8 cells/blastocyst). When PA oocytes were treated with LA, CB, and CD in combination with DMAP, blastocyst formation was significantly (P<0.05) improved by CB+DMAP (42.5%) compared to LA+DMAP (28.0%) and CD+DMAP (25.1%), but no significant differences were found in embryo cleavage (77.5~78.0%) and mean blastocyst cell number (33.6~35.0 cells) among the three groups. In SCNT, blastocyst formation was significantly (P<0.05) increased by post-activation treatment with LA+DMAP (32.9%) and CD+DMAP (35.0%) compared to CB+DMAP (22.0%) while embryo cleavage (85.5~85.7%) and blastocyst cell number (41.1~43.8 cells) were not influenced. All three treatments (LA, CB, and CD with DMAP) effectively inhibited pseudo-polar body extrusion in SCNT oocytes. The proportions of oocytes showing single pronucleus formation were 89.6%, 83.9%, and 93.3%, respectively with the increased tendency (P<0.1) by LA+DMAP and CD+ DMAP compared to CB+DMAP. Our results demonstrate that post-activation treatment with LA or CD in combination with DMAP improves pre-implantation development of SCNT embryos and the stimulating effect of cytoskeletal modifiers on embryonic development is differentially shown depending on the origin (PA or SCNT) of embryos in pigs.

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