Cytologic Aspects of Oral Squamous Cell Carcinoma in a Captive African Hedgehog (Altelerix albiventris)

고슴도치의 구강 편평상피암종의 세포학적 고찰 1증례

  • Cho, Hyang Mi (College of Veterinary Medicine, Chonbuk National University) ;
  • Choi, Ul Soo (College of Veterinary Medicine, Chonbuk National University) ;
  • Lee, Hae Beom (College of Veterinary Medicine, Chonbuk National University)
  • Accepted : 2013.06.07
  • Published : 2013.06.30

Abstract

A 3-year-old intact female hedgehog (Atelerix albiventris) was presented for evaluation of mandibular swelling. Fine needle aspiration of swollen mandibular was performed and smears were stained with Romanowsky type stain for cytological evaluation. Smears were highly cellular with predominance of variably shaped keratinized or non keratinized squamous cells with low numbers of spindloid to abnormally elongated cells. Cytologic impression was squamous cell carcinoma. The mass was surgically removed for histological examination. Microscopically tortuous and anastomosing delicate to broad pegs and nests of neoplastic squamous epithelial cells were supported by a moderate collagenous and spindloid fibroblast stroma. Tumor cells had moderate anisocytosis and mild anisokaryosis and range from moderately to well keratinized, with areas of intratumoral acantholysis accompanied by mixed stromal lymphoplasmacytic, neutrophilic inflammation. The mitotic index is 2-3 per high-power field. Tumor cells were expanding the subcutis subjacent to the layer of skeletal muscle and incorporating the osseous tissue fragments. The final diagnosis was squamous cell carcinoma. The patient survived three months after surgery without any further medical treatments.

3세령의 암컷 고슴도치(Atelerix albiventris)가 심하게 종대된 하악 병변의 평가를 위해 내원하였다. 세포학적 검사를 위해 세침흡인을 실시하였으며, 도말표본을 제작 후 로마노프스키식 염색으로 염색하였다. 세포학적 검사에서 원형을 포함한 다양한 모양의 편평상피세포가 주로 관찰되었으며, 일부 세포는 유사 방추 모양 또는 매우 길쭉한 모양을 띄고 있었다. 세포학적 소견은 편평상피암종이었으며, 조직검사를 위해 수술적으로 제거하였다. 조직병리상 악성 편평상피암종세포들이 중등도의 콜라겐과 방추형 섬유모세포로 이루어진 간질에 둘러싸여 있었으며, 인접한 골격근과 골조직으로의 침습이 관찰되었다. 종양세포는 중등도의 세포 대소부동과 핵 대소부동을 보였으며, 중등도 이상의 각화상태를 보였다. 종양의 중심부에서는 극세포해리가 있었으며 림프형질세포 및 호중구성 염증소견이 동반되었다. 유사 분열상은 고배율에서 2-3개가 관찰되었다. 이와 같은 소견에 기초해서 편평상피암종으로 진단하였으며, 보호자는 종괴의 수술적 제거 후 더이상의 치료를 원치 않아 바로 퇴원하였다. 환자는 추가 처치 없이 3개월 생존 후 폐사하였다.

Keywords

References

  1. Bernreuter DC. The Oropharynx and Tonsils. In: Diagnostic cytology and hematology of the dog and cat. Cowell RL, Tyler RD, Meinkoth JH, DeNicola DB, 3rd ed. St.Louis: Mosby 2008: 141-146.
  2. Gál J, Landauer K, Palade EA, Ivaskevics K, Rusvai M, Demeter Z. Squamous cell carcinoma and consequent otitis in a long-eared hedgehog (Hemiechinus auritus)-Case report. Acta Vet Hung 2009; 57: 69-73. https://doi.org/10.1556/AVet.57.2009.1.7
  3. Garma-Aviña A. The cytology of squamous cell carcinomas in domestic animals. J Vet Diagn Invest 1994; 6: 238-246. https://doi.org/10.1177/104063879400600216
  4. Goldschmidt MH, Hendrick MJ. Tumors of the Skin and Soft Tissues. In: Tumors in domestic animals, 4th eds. Meuten, DJ. Iowa State Press, Ames. 2003; 45-54.
  5. Gupta PK, Baloch ZW. Cytohistology: Essentials and basic concepts. Cambridge: Cambridge University Press. 2011: 95-123.
  6. Heatley JJ, Mauldin GE, Cho DY. A Review of Neoplasia in the Captive African Hedgehog (Atelerix albiventris). Semin Avian Exo Pet Med 2005; 14: 182-192. https://doi.org/10.1016/j.saep.2005.07.002
  7. Juan-Sallés C, Garner MM. Cytologic diagnosis of diseases of hedgehogs. Vet Clin North Am Exot Anim Pract 2007; 10: 51-59. https://doi.org/10.1016/j.cvex.2006.10.003
  8. Raskin RE. Skin and Subcutaneous Tissues. In: Canine and Feline cytology. A color atlas and interpretation guide. Raskin RE, Meyer D, 2nd ed. St. Louis: SAUNDERS. 2010: 26-76.
  9. Raymond J, Garner M. Spontaneous tumours in captive African hedgehogs (Atelerix albiventris): a retrospective study. J Comp Pathol 2001; 124: 128-133. https://doi.org/10.1053/jcpa.2000.0441
  10. Rivera R, Janovitz EB. Oronasal squamous cell carcinoma in an African hedgehog (Erinaceidae albiventris). J Wildl Dis 1992; 28: 148-150. 고슴도치의 구강 편평상피암종의 세포학적 고찰 1증례 조향미.최을수.이해범1 전북대학교 수의과대학 요 약: https://doi.org/10.7589/0090-3558-28.1.148