Pulmonary Aspergillosis Secondary to Open Fracture of Pneumatic Bone in a Brown Hawk Owl (Ninox scutulata)

솔부엉이의 함기골 개방골절에 의한 속발성 폐 아스퍼질러스 감염 증례

  • Han, Jae-Ik (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Lee, Young-Sun (Chungbuk Wildlife Center for Chungbuk) ;
  • Lee, Sook-Jin (Chungbuk Wildlife Center for Chungbuk) ;
  • Kang, Hyo-Min (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Jang, Hye-Jin (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University) ;
  • Chang, Dong-Woo (Laboratory of Medical Imaging, College of Veterinary Medicine, Chungbuk National University) ;
  • Na, Ki-Jeong (Veterinary Laboratory Medicine, College of Veterinary Medicine, Chungbuk National University)
  • 한재익 (충북대학교 수의과대학 진단검사의학) ;
  • 이영선 (충북 야생동물센터) ;
  • 이숙진 (충북 야생동물센터) ;
  • 강효민 (충북대학교 수의과대학 진단검사의학) ;
  • 장혜진 (충북대학교 수의과대학 진단검사의학) ;
  • 장동우 (충북대학교 수의과대학 영상진단의학) ;
  • 나기정 (충북대학교 수의과대학 진단검사의학)
  • Accepted : 2013.06.10
  • Published : 2013.06.30

Abstract

A flightless brown hawk owl (Ninox scutulata) weighing 180 g was rescued and referred to the teaching veterinary hospital with humerus open fracture. On physical examination and radiography, open fracture of the left humerus was found. After 3 days, orthopedic surgery was operated with intramedullary pins and wires. The bird died 2 days after surgery with anorexia. On necropsy, multiple green nodules with 2-3 mm in diameter were observed at the surface and inside of the left lung. Numerous conidial heads and spores were seen in the center of foci in the histopathological examination. The mycelia penetrated the surrounding pulmonary parenchyma, showing inflammation and necrosis. The fungus was isolated from the lung and cultured on Sabouraud Dextrose Agar at $30^{\circ}C$ for 7 days. The colony was blue-green color with a powdery surface. The fungus was identified as Aspergillus fumigatus by DNA analysis, including the internal transcribed spacer region, partial ${\beta}$-tubulin, and the calmodulin gene. This case was diagnosed as pulmonary aspergillosis secondary to open fracture of pneumatic bone in a brown hawk owl.

비행곤란을 나타내는 솔부엉이가 구조되어 충북대학교 동물의료센터에 의뢰되었다. 신체검사와 방사선 검사에서 좌측 상완골의 개방골절을 확인하였다. 3일 후 수내정과 와이어를 이용해 골절부 정복을 실시하였으나, 수술 후 2일 만에 급사하였다. 부검시 2~3 mm 크기의 녹색 결절이 왼쪽 폐의 표면과 내부에 다발성으로 발생하였다. 조직검사 결과 포자와 균사가 폐포에 다량 증식되고, 일부가 폐 실질에 침입하여 염증과 괴사를 유발하였다. 진균을 분리하여 Sabouraud dextrose 배지에 $30^{\circ}C$에서 7일간 배양한 결과, 청녹색의 밸벳 양상을 나타내는 곰팡이를 관찰하였다. ITS, ${\beta}$-tubulin 및 calmodulin 유전자를 증폭하여 동정한 결과 A. fumigatus로 확인되었다. 최종적으로 함기골 개방골절에 따른 이차적인 폐 아스퍼질러스증으로 사망한 것으로 진단하였다.

Keywords

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