Journal of agriculture & life science (농업생명과학연구)

Institute of Agriculture & Life Science, Gyeongsang National University (경상대학교 농업생명과학연구원)
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Control of Spinach Downy Mildew Using Essential Oil, Antagonistic Bacteria, Cooking Oil and Egg Yolk Mixture

식물정유, 길항세균 및 난황유를 이용한 시금치 노균병 방제

  • Received : 2011.12.24
  • Accepted : 2012.04.27
  • Published : 2012.04.30
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Abstract

In this study, we evaluated environmental-friendly disease control methods using essential oils, antagonistic bacteria, or cooking oil and egg yolk mixture (COY). Among the five tested plant essential oils, Eucalyptus approximans oil shown the highest effect to control of the downy mildew disease (60% disease control value). Interestingly enough, well known antagonistic bacteria against various fungal pathogens were appeared lower control efficiency than plant essential oils. In field conditions, the cooking oil and yolk mixture (COY) treatments shown significantly decreased the downy mildew incidence with 0.3% (disease conttrol value 84%) and 0.5% concentration (disease control value 91%). Our findings suggest that the COY has a great potential as the eco-friendly downy mildew disease control formulation.

본 연구는 친환경적으로 시금치 노균병을 방제하기 위하여 식물정유, 길항미생물 및 난황유의 병발생 억제효과를 검정하여 화학농약 대신에 이용할 수 있는 친환경제제로서의 가능성을 검토하였다. 5종의 식물정유 가운데 노균병 방제효과는 1회 처리구와 1주 간격 2회 처리구 모두 Eucalyptus approximans 오일의 병원균 억제효과가 60% 정도로 가장 높은 것으로 나타났다. 반면에 토양에서 선발된 길항균 세 종류의 시금치 노균병 방제효과는 모두 20% 이하로 식물정유 처리보다 낮게 나타났다. 난황유 0.3%와 0.5%농도에서 1주일간격 2회 처리구에서 병원균 억제율이 각각 84.5%와 91.5%로 억제되어 가장 효과가 우수한 것으로 나타났다.

Keywords

Acknowledgement

Supported by : 농림수산식품부

References

  1. Beatty P. H. and S. E. Jensen. 2002. Paenibacillus polymyxa produces fusaricidin-type antifungal antibiotics active against Leptosphaeria maculans, the causative agent of blackleg disease of canola. Can. J. Microbiol. 48: 159-169. https://doi.org/10.1139/w02-002
  2. Bergman, M., L. Varshavsky, H. E. Gottlieb, and S. Grossman, 2001. The antioxidant activity of aqueous spinach extract: chemical identification of active fractions. Phytochemistry. 58: 143-152. https://doi.org/10.1016/S0031-9422(01)00137-6
  3. Cava, R., E. Nowak, A. Taboada and F. Marin-Iniesta. 2007. Antimicrobial Activity of Clove and Cinnamon Essential Oils against Listeria monocytogenes in Pasteurized Milk. J. Food Prot. 70: 2757-2763. https://doi.org/10.4315/0362-028X-70.12.2757
  4. Chandha, S. L. 1996. Natural sources of antioxidants and their adequacy in diet to prevent atherosclerosis. Mediquest. 143: 337-351.
  5. Chitarra, G. S., P. Breeuwer, M. J. R. Nout, A. C. van Aelst, F. M. Rombouts and T. Abee. 2003. An antifungal compound produced by Bacillus subtilis YM 10-20 inhibits germination of Penicillium roqueforti conidiospores. J. Appl. Microbiol. 94: 159- 166. https://doi.org/10.1046/j.1365-2672.2003.01819.x
  6. Correll, J. C., T. E., Morelock, M. C., Black, S. T., Koike, L. P., Brandenberger, and F. J. Dainello. 1994. Economically important diseases of spinach. Plant Dis. 78: 653-660. https://doi.org/10.1094/PD-78-0653
  7. Gerster, H., 1993. Anticarcinogenic effect of common carotenoids. Int. J. Nutr. Res. 63: 93-121.
  8. Gong, M., J. D. Wang, J. Zhang, H. Yang, X. F. Lu, Y. Pei, and J. Q. Cheng. 2006. Study of the antifungal ability of Bacillus subtilis strain PY-1 in vitro and identification of its antifungal substance (Iturin A). Acta Biochim Biophys Sin. 38: 233-240. https://doi.org/10.1111/j.1745-7270.2006.00157.x
  9. Jee, H. J., C. K. Shim, K. Y. Ryu, and D. H. Choi, 2005. Effects of cooking oils on control of powdery mildew of cucumber caused by Sphaerotheca fuliginea. Plant Phytho. J. 21: 415.
  10. Jee, H. J., C. K. Shim, K. Y. Ryu, J. H. Park, B. M. Lee, D. H. Choi, and G. H. Ryu. 2009. Control of powdery and downy mildews of cucumber by using cooking oils and yolk mixture. Plant Pathol. J. 25: 280-285. https://doi.org/10.5423/PPJ.2009.25.3.280
  11. Jee, H. J., K. Y. Ryu, J. H. Park, D. H. Choi, G. H. Ryu, J. G. Ryu, and S. S. Shen. 2008. Effect of COY (Cooking Oil and Yolk mixture) and ACF (Air-circulation Fan) on Control of powdery mildew and production of organic lettuce. Res. Plant Dis. 14: 51-56. https://doi.org/10.5423/RPD.2008.14.1.051
  12. Kwon, J. H., C. K. Shim, H. J. Jee, and C. S. Park. 2009. Control of powdery mildew on solanaceous crops by using COY (Cooking Oil and Yolk Mixture) in the greenhouse. Res. Plant Dis. 15: 23-29. https://doi.org/10.5423/RPD.2009.15.1.023
  13. Lee, J. H., K. S. Han, Y. S. Kwon, D. K. Kim, and H. K. Kim. 2008. Control of paprika powdery mildew using cooking oil and yolk mixture. Res. Plant Dis. 14: 112-116. https://doi.org/10.5423/RPD.2008.14.2.112
  14. Leifert, C., H. Li, S. Chidburee, S. Hampson, S. Workman, D. Siqee, H. A. Epton, and A. Harbour. 1995. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. J. Appl. Bacteriol. 78: 97-108. https://doi.org/10.1111/j.1365-2672.1995.tb02829.x
  15. Seema, M. and N. S. Devaki 2010. Effect of some essential oils on Rhizoctonia solani Kuhn infecting flue-cured virginia tobacco J. Biopesticides. 3: 563- 566.
  16. Siripornvisal, S., W. Rungprom, and S. Sawatdikarn, 2009. Antifungal activity of essential oils derived from some medicinal plants against grey mould (Botrytis cinerea). As. J. Food Ag-Ind. 229-233.
  17. Smith, I. M. J. Dunez, R. A, Lelliott, D. H. Phillips, and S. A, Archer, 1988. European Handbook of Plant Diseases. Blackwell Scientific Publications. Oxford, UK.
  18. Walker, R., A. A. Powell, and B. Seddon. 1998. Bacillus isolates from the spermosphere of peas and dwarf French beans with antifungal activity against Botrytis cinerea and Pythium species. J. Appl. Microbiol. 84: 791-801. https://doi.org/10.1046/j.1365-2672.1998.00411.x
  19. Zennie T. M. and C. D. Ogzewalla. 1977. Ascorbic acid and vitamin A content of edible wild plants of Ohio and Kentucky. J. Econ. Bot. 31: 76-79. https://doi.org/10.1007/BF02860657