Polymer(Korea) (폴리머)

The Polymer Society of Korea (한국고분자학회)
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Synthesis of Homing Peptide-Immobilized Magnetite Nanoparticles through PEG Spacer and Their Biomedical Applications

PEG 스페이서를 통해 Homing 펩타이드를 고정화한 산화철 나노입자의 제조 및 생의학적 응용

  • Lee, Sang-Min (Department of Polymer Science & Engineering, Kyungpook National University) ;
  • Xing, Zhi-Cai (Department of Polymer Science & Engineering, Kyungpook National University) ;
  • Shin, Yong-Suk (Department of Polymer Science & Engineering, Kyungpook National University) ;
  • Gu, Tae-Hyung (Department of Polymer Science & Engineering, Kyungpook National University) ;
  • Lee, Byung-Heon (Department of Biochemistry, School of Medicine, Kyungpook National University) ;
  • Huh, Man-Woo (School of Textile and Fashion Technology, Kyungil University) ;
  • Kang, Inn-Kyu (Department of Polymer Science & Engineering, Kyungpook National University)
  • 이상민 (경북대학교 고분자공학과) ;
  • 싱즐차이 (경북대학교 고분자공학과) ;
  • 신용석 (경북대학교 고분자공학과) ;
  • 구태형 (경북대학교 고분자공학과) ;
  • 이병헌 (경북대학교 의과대학 생화학교실) ;
  • 허만우 (경일대학교 섬유패션학과) ;
  • 강인규 (경북대학교 고분자공학과)
  • Received : 2012.01.28
  • Accepted : 2012.03.21
  • Published : 2012.09.25
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Abstract

Iron oxides ($Fe_3O_4$) are metabolically secreted after endocytosed by cells, indicating no cytotoxicity. Therefore, they are widely used as a contrast agent before photographing of magnetic resonance imaging. In this study, iron oxide nanoparticles are synthesized by the co-precipitation method and subsequently immobilized with a homing peptide (AP), which specifically interacts with interleukin-4 receptor located on the membrane of endothelial and bladder cancer cells. The size of AP-immobilized iron oxide particle is about 39 nm. Intracellular uptake of the AP-immobilized iron oxide nanoparticles was investigated using bladder cancer cells and fibroblasts as the control. As the result, the nanoparticles are specificially uptaken by bladder cancer cells. However, the nanoparticles are not specificially uptaken by fibroblast. It could be said that the AP-immobilized iron oxide nanoparticles have a potential to be used as a contrast agent for early diagnosis of cancer.

산화철($Fe_3O_4$은 세포에 의해 섭취된 후 대사반응에 의해 분비되므로 세포독성을 나타내지 않는다. 따라서 산화철 나노입자는 MRI 촬영을 하기에 앞서 조영제로서 널리 사용되고 있다. 본 연구에서는 통상의 공침법으로 산화철 나노입자를 합성하고 폴리에틸렌글리콜을 스페이서로 하여 혈관내피세포 및 방광암 세포막의 IL-4 리셉터에 특이적으로 반응하는 homing 펩타이드(AP)를 고정화하였다. AP를 고정화한 산화철 나노입자의 크기는 수용액 상에서 약 39 nm이었다. 섬유아세포 및 방광암세포를 이용하여 AP고정화 산화철 나노입자의 uptake를 조사한 결과 섬유아세포에는 선택적 uptake를 발견할 수 없었으나 방광암세포에는 선택적으로 uptake됨을 알 수 있었다. 따라서 AP 고정화 산화철 나노입자는 조기 암진단용 조영제로서 가능성을 지니고 있다고 할 수 있다.

Keywords

Acknowledgement

Supported by : 한국연구재단

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