DOI QR코드

DOI QR Code

Influence of Pesticides and Environmentally Friendly Agricultural Materials used in Tomato Cultivation on the Pathogenicity of the Entomopathogenic Fungus, Beauveria bassiana

토마토재배에 사용하는 농약과 친환경농자재가 곤충 병원성 곰팡이 Beauveria bassiana의 병원성에 미치는 영향

  • Park, Jong-Ho (National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Sung-Jun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Eun-Jung (National Academy of Agricultural Science, Rural Development Administration) ;
  • Shim, Chang-Ki (National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Minho (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Min-Jeong (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, JeongJun (National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Yong-Ki (National Academy of Agricultural Science, Rural Development Administration)
  • 박종호 (농촌진흥청 국립농업과학원) ;
  • 홍성준 (농촌진흥청 국립농업과학원) ;
  • 한은정 (농촌진흥청 국립농업과학원) ;
  • 심창기 (농촌진흥청 국립농업과학원) ;
  • 이민호 (농촌진흥청 국립농업과학원) ;
  • 김민정 (농촌진흥청 국립농업과학원) ;
  • 김정준 (농촌진흥청 국립농업과학원) ;
  • 김용기 (농촌진흥청 국립농업과학원)
  • Received : 2012.08.02
  • Accepted : 2012.09.25
  • Published : 2012.12.01

Abstract

This study was conducted to observe the influence of chemical pesticides and environmentally friendly agricultural materials (EFAMs) used in tomato cultivation on the pathogenicity of the entomopathogenic fungus, Beauveria bassiana. B. bassiana mycelium didn't grow on PDA media containing 13 fungicides including chlorothalonil and colonies were not formed on PDA media containing 12 fungicides. B. bassiana mycelium grew and colonies were formed on all PDA media containing insecticides and EFAMs, but mycelial growth and colony formation on most PDA media were significantly inhibited compared to the control. The insecticidal activity of B. bassiana against Trialeurodes vaporariorum was decreased when fungicides (polyoxin B, mandipropamid) and EFAMs containing sulfur were added, but insecticides (pyridaben, dinotefuran) and EFAMs originated from plant extracts did not have any influence on the insecticidal activity of B. bassiana. The pathogenicity of a mixture of B. bassiana and polyoxin B against T. vaporariorum was lower than that of B. bassiana alone under greenhouse conditions.

토마토 재배에 사용되는 농자재를 이용하여 곤충 병원성 곰팡이인 Beauveria bassiana의 병원성에 끼치는 영향을 조사하였다. Chlorothalonil 등 13종의 살균제 첨가 배지에서는 B. bassiana 균사가 전혀 생장하지 못하였고 chlorothalonil 등 12종의 살균제 첨가 배지에서 균총이 형성되지 않았다. 살충제와 친환경자재를 첨가한 모든 배지에서 균사가 생장하고 포자가 형성되었지만 대부분 배지에서 무처리와 비교했을 때 유의차를 보였다. 온실가루이에 대한 살충력은 B. bassiana 단독처리일 때보다 화학살균제(polyoxin B, mandipropamid)와 친환경농자재(sulfur) 혼합시 떨어졌으며 화학살충제(pyridaben, dinotefuran)와 식물추출물 유래 친환경농자재(pyrethrum) 혼합시에는 유의차가 없었으며 온실에서 살균제인 polyoxin B의 혼합으로 온실가루이에 대한 B. bassiana의 감염력이 떨어지는 것을 확인하였다.

Keywords

References

  1. Amutha, M., Banu, J.G., Surulivelu T., Gopalakrishnan, N., 2010. Effect of commonly used insecticides on the growth of white muscardine fungus, Beauveria bassiana under laboratory conditions. J. Biopest. 3, 143-146.
  2. Barranco-Florido, J.E., Alatorre-Rosas, R., Gutidrrez-Rojas M., Viniegra-Gonzalez. G., 2002. Criteria for the selection of strains of entomo-pathogenic fungi Verticillium lecanii for solid state cultivation. Enzyme Microb. Technol. 30, 910-915. https://doi.org/10.1016/S0141-0229(02)00032-7
  3. Clark, R.A., Casagrande, R.A., Wallace, D.B., 1982. Influence of pesticides on Beauveria bassiana, a pathogen of the colorado potato beetle. Environ. Entomol. 11, 67-70. https://doi.org/10.1093/ee/11.1.67
  4. Faria, M., Wraight, S.P., 2001. Biological control of Bemisia tabaci with fungi. Crop Protect. 20, 767-778. https://doi.org/10.1016/S0261-2194(01)00110-7
  5. Hassan, A.E.M., Charnley, A.K., 1989. Ultrastructural-study of the penetration by Metarhizium anisopliae through dimilin-affected cuticle of Manduca sexta. J. Invertebr. Pathol. 54, 117-124. https://doi.org/10.1016/0022-2011(89)90148-1
  6. Islam, M.T., Castle, S.J., Ren, S., 2010a. Compatibility of the insect pathogenic fungus Beauveria bassiana with neem against sweetpotato whitefly, Bemisia tabaci, on eggplant. Entomol. Exp. Appl. 134, 28-34. https://doi.org/10.1111/j.1570-7458.2009.00933.x
  7. Islam, M.T., Olleka A., Ren, S., 2010b. Influence of neem on susceptibility of beauveria bassiana and investigation of their combined efficacy against sweetpotato whitefly, Bemisia tabaci on eggplant. Pestic. Biochem. Physiol. 98, 45-49. https://doi.org/10.1016/j.pestbp.2010.04.010
  8. Islam, M.T., Omar, D., Latif, M., Morshed, M.M., 2011. The integrated use of entomopathogenic fungus, Beauveria bassiana with botanical insecticide, neem against Bemisia tabaci on eggplant. Afr. J. Microbiol. Res. 5, 3409-3413.
  9. Jaros-Su, J., Groden, E., Zhang, J., 1999. Effects of selected fungicides and the timing of fungicide application on Beauveria bassiana-induced mortality of the colorado potato beetle (Coleoptera: Chrysomelidae). Biol. Control. 15, 259-269. https://doi.org/10.1006/bcon.1999.0724
  10. Kim, Y.K., 2011. Development on the Plants of Solanaceae (Pepper, Eggplant, Tomato). KIC News. 14, 28.
  11. Lee, S.M., Lee, D.W., Choo, H.Y., Park, Y.D., 1997. Effects of pesticides on the pathogenicity of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae. Korean J. Appl. Entomol. 36, 179-184.
  12. Loria, R., Galaini, S., Roberts, D.W., 1983. Survival of inoculum of the entomopathogenic fungus Beauveria bassiana as influenced by fungicides. Environ. Entomol. 12, 1724-1726. https://doi.org/10.1093/ee/12.6.1724
  13. Omer, A.D., Leigh, T.F., Granett, J., 1992. Insecticide resistance in field populations of greenhouse whitefly (Homoptera: Aleyrodidae) in the San Foaquin Valley (California) cotton cropping system. J. Econ. Entomol. 85, 21-27. https://doi.org/10.1093/jee/85.1.21
  14. Quintela, E.D. McCoy, C.W., 1998. Synergistic effect of imidacloprid and two entomopathogenic fungi on the behavior and survival of larvae of Diaprepes abbreviatus (Coleoptera, Curculionidae) in Soil. J. Econ. Entomol. 91, 110-122. https://doi.org/10.1093/jee/91.1.110
  15. SAS Institute, 2004. SAS User's Guide: Statistics. SAS Institute, Cary, NC
  16. Shi, Z., Li, M., Zhang, L., 2006. Effects of nutrients on germination of Verticillium lecanii (=Lecanicillium sp.) conidia and infection of green-house whitefly, (Trialeurodes vaporariorum). Biocontrol Sci. Tech. 16, 599-606. https://doi.org/10.1080/09583150500532832
  17. Tedders, W.L., 1981. In vitro inhibition of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae by six fungicides used in pecan culture. Environ. entomol. 10, 346-349. https://doi.org/10.1093/ee/10.3.346
  18. Vandenberg, J.D., Shelton, A.M., Wilsey, W.T., Ramos, M., 1998. Assessment of Beauveria bassiana sprays for control of diamondback moth (Lepidoptera: Plutellidae) on crucifers. J. Econ. Entomol. 91, 624-630. https://doi.org/10.1093/jee/91.3.624