The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Effect of Chungganhaeju-hwan in Ethanol-induced Neuronal Cell Damage

청간해주환(淸肝解酒丸)의 알코올 유도 뇌신경세포 손상에 대한 보호 효과

  • Ju, Mi-Sun (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Kim, Hyo-Geun (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University) ;
  • Cho, Hae-Jeong (Department of Life and Nanopharmaceutical Science, Kyung Hee University) ;
  • Sim, Jae-Jong (Dasarang Central Hospital.Oriental Hospital) ;
  • Jeon, Yong-Jun (Dasarang Central Hospital.Oriental Hospital) ;
  • Oh, Myung-Sook (Department of Oriental Pharmaceutical Science, College of Pharmacy, Kyung Hee University)
  • 주미선 (경희대학교 약학대학 한약학과) ;
  • 김효근 (경희대학교 약학대학 한약학과) ;
  • 조해정 (경희대학교 나노의약생명과학과) ;
  • 심재종 (다사랑병원.다사랑한방병원(중앙)) ;
  • 전용준 (다사랑병원.다사랑한방병원(중앙)) ;
  • 오명숙 (경희대학교 약학대학 한약학과)
  • Received : 2011.08.15
  • Accepted : 2011.09.17
  • Published : 2011.09.30
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Abstract

Objectives : In this study, we evaluated the effect of Chungganhaeju-hwan(CGHJH) on hydrogen peroxide($H_2O_2$)-induced and ethanol(EtOH)-induced neuronal damage in vitro and in vivo, respectively. Methods:We carried out the anti-oxidant effects of CGHJH against hydrogen peroxide($H_2O_2$)-induced toxicity in HT22 and PC12 cells using thiazolyl blue tetrazolium bromide. Then, to investigate the protective effect on CGHJH against EtOH-induced memory impairment and hippocampal cell damage in male ICR mice, we performed novel object recognition test(NORT), and analysed the brain tissues after immunohistochemistry and western blotting. Results:CGHJH showed protective effect from $H_2O_2$-induced cell toxicity at doses of $1\sim100{\mu}g$/mL in both HT22 and PC12 cells. CGHJH had also recovery effect from EtOH-induced memory impairment in ICR mice from NORT and it protected hippocampal cells against EtOH toxicity in the result of cresyl violet and NeuN immunoreactivity. Conclusion : These results demonstrate that CGHJH has protective effect in neuronal cells against $H_2O_2$ and EtOH toxicities and this effect could be a main role of recovery effect on EtOH-induced memory loss.

Keywords

References

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