The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Hepatoprotective Activity of Spatholobi Caulis Water Extract against Acetaminophen-induced Toxicity in Rats

아세트아미노펜으로 유도된 간독성에 대한 계혈등 물추출물의 간세포 보호효과

  • Lee, In-Woo (Department of Oriental Internal Medicine, Daegu Haany University) ;
  • Choi, Hong-Sik (Department of Oriental Internal Medicine, Daegu Haany University) ;
  • Kim, Seung-Mo (Department of Oriental Internal Medicine, Daegu Haany University)
  • 이인우 (대구한의대학교 한의학과 간계내과학교실) ;
  • 최홍식 (대구한의대학교 한의학과 간계내과학교실) ;
  • 김승모 (대구한의대학교 한의학과 간계내과학교실)
  • Received : 2011.07.25
  • Accepted : 2011.09.17
  • Published : 2011.09.30
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Abstract

Objectives : The present study was evaluated the protective roles of Spatholobi Caulis in hepatotoxic rats due to APAP overdose. Methods : In experiments, rats were orally administrated with the aqueous extract of Spatholobi Caulis (SCE; 50, 100 mg/kg) for 4 days and then, orally gavage with APAP (1.2 g/kg) to induce acute liver damage. Results : Oral injection of APAP caused severe hepatic injury. Plasma ALT, AST and LDH levels were significantly elevated, but SCE significantly decreased ALT, AST and LDH to the normal level. In histopathological analysis, peripheral hemorrhage around portal triads and central necrosis around central veins were founded after APAP treatment. However, these histopathological changes were recovered by SCE pretreatment. SCE also decreased the percentage of generative hepatic regions (%/$mm^2$ hepatic parenchyma), the numbers of inflammatory cells (cells/$mm^2$ hepatic parenchyma) and the number of degenerative hepatic cells (N/100 hepatic cellls) which were significantly elevated after APAP injection. Furthermore, SCE down-regulated the contents of hepatic MDA and up-regulated hepatic GSH. SCE also inhibited the decrease in the expression of pro-caspase-3 by APAP treatment. Conclusions : Collectively, these data indicate that SCE protected APAP-induced hapatic damages through antioxidative and anti-apoptotic process. These findings the significant therapeutic potential of SCE during APAP-induced liver injury.

Keywords

References

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