Korean Chemical Engineering Research

  • 제49권5호
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  • Pages.628-632
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  • 2011
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  • 0304-128X(pISSN)
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  • 2233-9558(eISSN)
한국화학공학회 (The Korean Institute of Chemical Engineers)
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L-리보스 생산을 위한 L-아라비노스의 에피머반응

Epimerization of L-Arabinose for Producing L-Ribose

  • Jeon, Young Ju (Department of Chemical Engineering Chungnam National University) ;
  • Song, Sung Moon (Department of Chemical Engineering Chungnam National University) ;
  • Lee, Chang Soo (Department of Chemical Engineering Chungnam National University) ;
  • Kim, In Ho (Department of Chemical Engineering Chungnam National University)
  • 발행 : 2011.10.01
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초록

L-리보스는 항바이러스 약의 출발물질로 근래 관심의 대상이다. 이 물질은 에피머 반응에 의해 L-아라비노스로 부터 얻을 수 있다. 몰리브덴 산화물이나 몰리브덴산 촉매 그리고 메탄올/물 용액에서 에피머 반응을 수행하였다. 반응 온도, 메탄올 분율, 촉매종류를 선정하여 최적 반응을 찾고자 하였다. 이온교환크로마토그래피를 이용하여 에피머 반응물을 분리하였고 L-리보스 HPLC 크로마토그램에서 반응수율을 계산하였다. Sodex 이온교환 HPLC 칼럼과 Phenomenex $NH_2$ HPLC 칼럼을 분석의 편의성 면에서 비교하였다. 20% 메탄올, $60^{\circ}C$, 그리고 40 g/L 몰리브덴산 조건에서 21% 최대수율을 얻었다.

L-ribose has recently attracted interest as a starting material for antiviral drug. It could be obtained from L-arabinose by epimerization reaction. Epimerization reaction was carried out with molybdenium oxide or molybdic acid catalyst and methanol/water solution. Reaction temperature, methanol percentage, and catalyst kind were selected to find an optimum reaction condition. Ion exhange chromatography was used for separating epimerization reaction mixture, and then HPLC chromatogram of L-ribose fraction obtained to calculate the yield of the reaction. Shodex ion exchange HPLC column(Model SC1011) and Phenomenex Luna $NH_2$ HPLC column were compared to employ a convenient HPLC analysis. It was found that the usage of 20% methanol, $60^{\circ}C$, and 40 g/L molybdic acid gives the best reaction condition with a yield of 21%.

키워드

참고문헌

  1. Byun, Y. R., "New Thermophilic L-Ribose Isomerase and its Application," KR10-0809445(2008).
  2. Jeon, Y. J. and Kim, I. H., "Determination of Adsorption Isotherms and Separation of L-Arabinose and D-Ribose in Cation Exchange Chromatography and HPLC," Kor. J. Biotechnol. Bioeng., 23, 31-36(2008).
  3. Okano, K., "Synthesis and Pharmaceutical Application of LRobose," Tetrahedron, 65, 1937-1949(2009). https://doi.org/10.1016/j.tet.2008.11.047
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  5. Zakaria, A. S., Tsuyoshi, S., Shakhawat, H., Masaru, U., Goro, T. and Ken, I., "Biochemical Preparation of L-Ribose and L-Arabinose from Ribitol," J. Biosci. Bioeng., 88, 444-448(1999). https://doi.org/10.1016/S1389-1723(99)80225-4