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http://dx.doi.org/10.9713/kcer.2011.49.5.628

Epimerization of L-Arabinose for Producing L-Ribose  

Jeon, Young Ju (Department of Chemical Engineering Chungnam National University)
Song, Sung Moon (Department of Chemical Engineering Chungnam National University)
Lee, Chang Soo (Department of Chemical Engineering Chungnam National University)
Kim, In Ho (Department of Chemical Engineering Chungnam National University)
Publication Information
Korean Chemical Engineering Research / v.49, no.5, 2011 , pp. 628-632 More about this Journal
Abstract
L-ribose has recently attracted interest as a starting material for antiviral drug. It could be obtained from L-arabinose by epimerization reaction. Epimerization reaction was carried out with molybdenium oxide or molybdic acid catalyst and methanol/water solution. Reaction temperature, methanol percentage, and catalyst kind were selected to find an optimum reaction condition. Ion exhange chromatography was used for separating epimerization reaction mixture, and then HPLC chromatogram of L-ribose fraction obtained to calculate the yield of the reaction. Shodex ion exchange HPLC column(Model SC1011) and Phenomenex Luna $NH_2$ HPLC column were compared to employ a convenient HPLC analysis. It was found that the usage of 20% methanol, $60^{\circ}C$, and 40 g/L molybdic acid gives the best reaction condition with a yield of 21%.
Keywords
L-Ribose; Epimerization; L-Arabinose;
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  • Reference
1 Byun, Y. R., "New Thermophilic L-Ribose Isomerase and its Application," KR10-0809445(2008).
2 Jeon, Y. J. and Kim, I. H., "Determination of Adsorption Isotherms and Separation of L-Arabinose and D-Ribose in Cation Exchange Chromatography and HPLC," Kor. J. Biotechnol. Bioeng., 23, 31-36(2008).
3 Okano, K., "Synthesis and Pharmaceutical Application of LRobose," Tetrahedron, 65, 1937-1949(2009).   DOI   ScienceOn
4 Seo, M. J., An, J. H., Shim, J. H. and Kim, G. C., "One-pot Inversion of D-Mannono-1,4-Lactone for the Practical Synthesis of L-Ribose," Tetrahedron Letters, 44, 3051-3052(2003).   DOI   ScienceOn
5 Zakaria, A. S., Tsuyoshi, S., Shakhawat, H., Masaru, U., Goro, T. and Ken, I., "Biochemical Preparation of L-Ribose and L-Arabinose from Ribitol," J. Biosci. Bioeng., 88, 444-448(1999).   DOI   ScienceOn