Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Morphological Changes Associated with the Antibacterial Action of Silver Ions against Bovine Mastitis Pathogens

은 이온의 항균효과에 대한 소 유방염 원인균의 형태학적 변화

  • Kang, Seog-Jin (National Institute of Animal Science, Rural Development Administration) ;
  • Seol, Jae-Won (Center for Healthcare Technology Development, Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University) ;
  • Hur, Tai-Young (National Institute of Animal Science, Rural Development Administration) ;
  • Jung, Young-Hun (National Institute of Animal Science, Rural Development Administration) ;
  • Choe, Chang-Yong (National Institute of Animal Science, Rural Development Administration) ;
  • Park, Sang-Youel (Center for Healthcare Technology Development, Bio-Safety Research Institute, College of Veterinary Medicine, Chonbuk National University)
  • 강석진 (농촌진흥청 국립축산과학원) ;
  • 설재원 (헬스케어 기술개발 사업단, 전북대학교 수의과대학 생체안전성 연구소) ;
  • 허태영 (농촌진흥청 국립축산과학원) ;
  • 정영훈 (농촌진흥청 국립축산과학원) ;
  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 박상열 (헬스케어 기술개발 사업단, 전북대학교 수의과대학 생체안전성 연구소)
  • Accepted : 2011.12.15
  • Published : 2011.12.31
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Abstract

Silver has potent antibacterial activity against a variety of bacteria while maintaining low toxicity in mammalian cells. This study was conducted to investigate the possible mechanism underlying the bactericidal effects of silver ions against bovine mastitis pathogens using electron microscopy. We used two different bacterial strains, Escherichia coli and Staphylococcus aureus, which are primarily responsible for the majority of bovine mastitis cases. Interaction between the bacteria and silver ions (50 ${\mu}g/mL$, 2 hours) were studied using energy-filtering transmission electron microscopy (EFTEM). EFTEM images showed that E. coli and S. aureus cells treated with the silver ions had distorted plasma membranes, silver ions attached to the outer membranes, scattered electron-light material, and leakage of cell contents from disrupted cell membranes.

은 이온(silver ion)은 동물세포에 낮은 독성을 가지면서도 다양한 종류의 세균들에 대해 강력한 항균력을 가지고 있다. 본 연구에서는 젖소 유방염 원인균들에 대한 은 이온의 항균효과를 전자현미경(TEM)을 통해 조사하였다. 본 실험에 사용된 균주는, 젖소 유방염의 주요 원인균인 Escherichia coli 와 Staphylococcus aureus를 사용하였다. 유방염 원인균에 대한 은 이온의 항균효과를 관찰하기 위해 50 ${\mu}g/mL$의 은 이온을 2시간 동안 세균에 노출시킨 후 전자현미경(Energy-Filtering Transmission Electron Microscopy, EFTEM) 을 이용하여 은 이온에 의한 세균의 형태 변화를 확인하였다. 은 이온 처리 후 전자현미경 촬영 결과 E. coli 와 S. aureus의 세포막이 변형되고, 세포 외부에 침착된 은 이온과, electron-light 모습으로 세포내 분산된 은 이온을 관찰 할 수 있었으며, 결과적으로 E. coli 와 S. aureus의 세포막이 파괴되어, 세포내용물이 외부에 누출됨으로써 세균이 사멸되는 것을 확인하였다.

Keywords

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