Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Comparison of tdcA Expression Between Escherichia coli and Salmonella enterica Serovar Typhimurium

  • Kim, Min-Jeong (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Lim, Sang-Yong (Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute) ;
  • Ryu, Sang-Ryeol (Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, and Center for Agricultural Biomaterials, Seoul National University)
  • Received : 2010.10.11
  • Accepted : 2010.12.25
  • Published : 2011.03.28
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Abstract

Both Escherichia coli (E. coli) and Salmonella enterica serovar Typhimurium (S. Typhimurium) have a tdc operon that encodes enzymes involved in a metabolic pathway for the degradation of L-serine and L-threonine. However, S. Typhimurium does not have the tdcR gene, which is a positive regulator in E. coli. In the present study, transcriptional analysis revealed that tdcA expression in E. coli is higher under anaerobic than aerobic growth conditions, but the opposite is true in S. Typhimurium. Interestingly, a tdcR mutant strain of E. coli showed a similar expression pattern to that observed in S. Typhimurium and was also induced by anaerobic shock. These results suggest that the induction of tdcA expression by anaerobic conditions is observable when tdcA expression is low owing to the absence of TdcR.

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References

  1. Datsenko, K. A. and B. L. Wanner. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acac. Sci. USA 97: 6640-6645. https://doi.org/10.1073/pnas.120163297
  2. Eswarappa, S. M., G. Karnam, A. G. Nagarajan, S. Chakraborty, and D. Chakravortty. 2009. lac Repressor is an antivirulence factor of Salmonella enterica: Its role in the evolution of virulence in Salmonella. PLoS ONE 4: e5789. https://doi.org/10.1371/journal.pone.0005789
  3. Ganduri, Y. L., S. R. Sadda, M. W. Datta, R. K. Jambukeswaran, and P. Datta. 1993. TdcA, a transcriptional activator of the tdcABC operon of Escherichia coli, is a member of the LysR family of proteins. Mol. Gen. Genet. 240: 395-402.
  4. Goss, T. J., H. P. Schweizer, and P. Datta. 1988. Molecular characterization of the tdc operon of Escherichia coli K-12. J. Bacteriol. 170: 5352-5359. https://doi.org/10.1128/jb.170.11.5352-5359.1988
  5. Hagewood, B. T., Y. L. Ganduri, and P. Datta. 1994. Functional analysis of the tdcABC promoter of Escherichia coli: Roles of TdcA and TdcR. J. Bacteriol. 176: 6214-6220. https://doi.org/10.1128/jb.176.20.6214-6220.1994
  6. HeBlinger, C., S. A. Fairhurst, and G. Sawers. 1998. Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate. Mol. Microbiol. 27: 477-492. https://doi.org/10.1046/j.1365-2958.1998.00696.x
  7. Hobert, E. H. and P. Datta. 1983. Synthesis of biodegradative threonine dehydratase in Escherichia coli: Role of amino acids, electron acceptors, and certain intermediary metabolites. J. Bacteriol. 155: 586-592.
  8. Kim, M., S. Lim, and S. Ryu. 2008. Molecular analysis of the Salmonella Typhimurium tdc operon regulation. J. Microbiol. Biotechnol. 18: 1024-1032.
  9. Kim, M., S. Lim, D. Kim, H. E. Choy, and S. Ryu. 2009. A tdcA mutation reduces the invasive ability of Salmonella enterica serovar Typhimurium. Mol. Cells 28: 389-395. https://doi.org/10.1007/s10059-009-0133-9
  10. Lim, S., H. Seo, H. Yoon, S. Choi, S. Heu, and S. Ryu. 2003. Molecular analysis of Salmonella enterotoxin gene expression. J. Microbiol. Biotechnol. 13: 598-606.
  11. Lim, S., K. Yong, and S. Ryu. 2005. Analysis of Salmonella pathogenicity island 1 expression in response to the changes of osmolarity. J. Microbiol. Biotechnol. 15: 175-182.
  12. Lim, S., M. Kim, J. Choi, and S. Ryu. 2010. A mutation in tdcA attenuates the virulence of Salmonella enterica serovar Typhimurium. Mol. Cells 29: 509-517. https://doi.org/10.1007/s10059-010-0063-6
  13. Maddocks, S. E. and P. C. F. Oyston. 2008. Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins. Microbiology 154: 3609-3623. https://doi.org/10.1099/mic.0.2008/022772-0
  14. Maurelli, A. T. 2007. Black holes, antivirulence genes, and gene inactivation in the evolution of bacterial pathogens. FEMS Microbiol. Lett. 267: 1-8. https://doi.org/10.1111/j.1574-6968.2006.00526.x
  15. Phillips, A. T., R. M. Egan, and B. Lewis. 1978. Control of biodegradative threonine dehydratase inducibility by cyclic AMP in energy-restricted Escherichia coli. J. Bacteriol. 135: 828-840.
  16. Sawers, G. 1998. The anaerobic degradation of L-serine and L-threonine in enterobacteria: Networks of pathways and regulatory signals. Arch. Microbiol. 171: 1-5. https://doi.org/10.1007/s002030050670
  17. Sawers, G. 2001. A novel mechanism controls anaerobic and catabolite regulation of the Escherichia coli tdc operon. Mol. Microbiol. 39: 1285-1298. https://doi.org/10.1111/j.1365-2958.2001.02316.x
  18. Schweizer, H. P. and P. Datta. 1989. Identification and DNA sequence of tdcR, a positive regulatory gene of the tdc operon of Escherichia coli. Mol. Gen. Genet. 218: 516-522. https://doi.org/10.1007/BF00332418
  19. Sumantran, V. N., A. J. Tranguch, and P. Datta. 1989. Increased expression of biodegradative threonine dehydratase of Escherichia coli by DNA gyrase inhibitors. FEMS Microbiol. Lett. 65: 37-40. https://doi.org/10.1111/j.1574-6968.1989.tb03593.x
  20. Wu, Y. and P. Datta. 1992. Integration host factor is required for positive regulation of the tdc operon of Escherichia coli. J. Bacteriol. 174: 233-240. https://doi.org/10.1128/jb.174.1.233-240.1992
  21. Wu, Y. and P. Datta. 1995. Influence of DNA topology on expression of the tdc operon in Escherichia coli K-12. Mol. Gen. Genet. 247: 764-767. https://doi.org/10.1007/BF00290409
  22. Wu, Y., R. V. Patil, and P. Datta. 1992. Catabolite gene activator protein and integration host factor act in concert to regulate tdc operon expression in Escherichia coli. J. Bacteriol. 174: 6918-6927. https://doi.org/10.1128/jb.174.21.6918-6927.1992

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