한국임상수의학회지 (Journal of Veterinary Clinics)

  • 제27권4호
  • /
  • Pages.311-314
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  • 2010
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  • 1598-298X(pISSN)
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  • 2384-0749(eISSN)
한국임상수의학회 (The Korean Society of Veterinary Clinics)
권호 표지

Bartonella Henselae 감염 후의 개 말초혈액단핵구에서의 사이토카인 양상

Cytokine Production in Canine PBMC after Bartonella Henselae Infection

  • 최은화 (삼성생명과학연구소 실험동물연구센터) ;
  • 이종화 (서울대학교 수의과대학 내과학교실) ;
  • 구혜정 (서울대학교 수의과대학 미생물학교실) ;
  • 박용호 (서울대학교 수의과대학 미생물학교실) ;
  • 윤화영 (서울대학교 수의과대학 내과학교실)
  • Choi, Eun-Wha (Laboratory Animal Research Center, Samsung Biomedical Research Institute) ;
  • Lee, Jong-Hwa (KRF Zoonotic Disease Priority Research Institute, Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Koo, Hye-Cheong (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Park, Yong-Ho (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Youn, Hwa-Young (KRF Zoonotic Disease Priority Research Institute, Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University)
  • 심사 : 2010.08.03
  • 발행 : 2010.08.30
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초록

Bartonella henselae 는 고양이할큄병의 원인체이다. 고양이가 Bartonella spp.의 주된 보유숙주이기는 하지만, 최근 애완견의 할큄에 의한 고양이할큄병 발생이 보고되었다. 8두의 개에 1 ml의 인산완충식염수에 부유한 $2{\times}10^8CFU$의 B. henselae Houston-1을 0일에 피내주사하고, 동량을 21, 28, 36, 58, 64일째에 추가로 피내주사 하였다. B. henselae 감염을 nested PCR을 통해 확인하였다. B. henselae-PCR 양성군이 음성군에 비해 B. henselae로 말초혈액단 핵구를 자극한 후 얻은 배양상청액에서의 IFN-$\gamma$ 농도가 유의성 있게 높았다. B. henselae자극시, Th1활성을 보이는 개 말초혈액단핵구의 면역양상은 Th2활성을 보인다고 알려진 고양이와는 다른 것으로 보인다.

Bartonella henselae is the causative agent of cat scratch disease. Although cats are the main zoonotic reservoirs of Bartonella spp., unusual cases of cat scratch disease caused by a domestic dog scratch have been recently reported. For the in vivo B. henselae infection, eight dogs were inoculated intradermally with $2{\times}10^8CFU$ of B. henselae Houston-1 suspended in 1 ml of phosphate buffered saline on day 0 and subsequent injections of the same amount given intradermally on days 21, 28, 36, 58 and 64. After in vivo canine B. henselae infection was confirmed by nested PCR, the IFN-$\gamma$ levels of the culture supernatant of PBMC stimulated with B. henselae was significantly higher in the B. henselae-PCR positive group than the B. henselae-PCR negative group. Our results showed that the canine immune responses against B. henselae were different from those of cats. Th1 activation by B. henselae stimulation was characterized in dog peripheral blood mononuclear cells, whereas Th2 activation was reported in B. henselae-infected cats.

키워드

참고문헌

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