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Cytokine Production in Canine PBMC after Bartonella Henselae Infection  

Choi, Eun-Wha (Laboratory Animal Research Center, Samsung Biomedical Research Institute)
Lee, Jong-Hwa (KRF Zoonotic Disease Priority Research Institute, Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University)
Koo, Hye-Cheong (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University)
Park, Yong-Ho (Department of Veterinary Microbiology, College of Veterinary Medicine, Seoul National University)
Youn, Hwa-Young (KRF Zoonotic Disease Priority Research Institute, Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Veterinary Clinics / v.27, no.4, 2010 , pp. 311-314 More about this Journal
Abstract
Bartonella henselae is the causative agent of cat scratch disease. Although cats are the main zoonotic reservoirs of Bartonella spp., unusual cases of cat scratch disease caused by a domestic dog scratch have been recently reported. For the in vivo B. henselae infection, eight dogs were inoculated intradermally with $2{\times}10^8CFU$ of B. henselae Houston-1 suspended in 1 ml of phosphate buffered saline on day 0 and subsequent injections of the same amount given intradermally on days 21, 28, 36, 58 and 64. After in vivo canine B. henselae infection was confirmed by nested PCR, the IFN-$\gamma$ levels of the culture supernatant of PBMC stimulated with B. henselae was significantly higher in the B. henselae-PCR positive group than the B. henselae-PCR negative group. Our results showed that the canine immune responses against B. henselae were different from those of cats. Th1 activation by B. henselae stimulation was characterized in dog peripheral blood mononuclear cells, whereas Th2 activation was reported in B. henselae-infected cats.
Keywords
Bartonella henselae; dog; cytokine; cat scratch disease; Th1 immunity;
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