The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Comparative Study of 25 Herbal Formulas on Anti-Inflammatory Effect

한약 처방 25종에 대한 항염증 효능 비교 연구

  • Lee, Jin-Ah (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Jung, Da-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Ho-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Lee, Jun-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Huang, Dae-Sun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine) ;
  • Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
  • 이진아 (한국한의학연구원 한약 EBM 연구센터) ;
  • 하혜경 (한국한의학연구원 한약 EBM 연구센터) ;
  • 정다영 (한국한의학연구원 한약 EBM 연구센터) ;
  • 이호영 (한국한의학연구원 한약 EBM 연구센터) ;
  • 이준경 (한국한의학연구원 한약 EBM 연구센터) ;
  • 황대선 (한국한의학연구원 한약 EBM 연구센터) ;
  • 신현규 (한국한의학연구원 한약 EBM 연구센터)
  • Received : 2010.07.27
  • Accepted : 2010.08.10
  • Published : 2010.08.31
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Abstract

Purpose: To provide the information of efficacy for herbal formulas of high frequency, it was evaluated the anti-inflammatory effect. In many studies, plantderived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of herbal formulas of high frequency on LPS-stimulated RAW 264.7 cells. Methods: Anti-inflammatory activity was investigated in 25 herbal formula extracts in vitro and in vivo. To investigate the anti-inflammatory effect in vitro model, using LPS-stimulated macrophages, RAW 264.7 cell line. The productions of nitric oxide(NO), prostaglandin(PG)$E_2$, interleukin(IL)-6 and tumor necrosis factor(TNF)-$\alpha$ were examined in RAW 264.7 cells, in the presence of the herbal formulas. RAW 264.7 cells were incubated with LPS $1\;{\mu}g/mL$ and herbal formulas for 18 hours. As an in vivo, using a rat model of carrageenin-induced paw edema. The paw volume was measured at 2 and 4 hours following carrageenin-induced paw edema in rats. Results: 8 kinds of herbal formula inhibited NO production by LPS-stimulated in some concentration, but the effect of NO inhibition is weak. 12 kinds of herbal formula inhibited $PGE_2$ production by LPS-stimulated over the 30%. Among them Gumiganghwal-tang, Sagunja-tang, Samchulkunbi-tang, Insampaedok-san and Hwangryunhaedok-tang inhibited IL-6 production by LPS-stimulated but TNF-$\alpha$ was not inhibited. 12 kinds of herbal formula reduced the carrageenin-induced paw edema in rats. Particularly, 3 kinds of herbal formula(Gumiganghwal-tang, Ssanghwa-tang and Soshiho-tang) were better than indomethacin. Conclusion: These results suggest that Gumiganghwal-tang, Sangunja-tang, Samchulkunbi-tang, Insampaedok-san and Hwangryunhaedok-tang have antiinflammatory activity.

Keywords

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