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http://dx.doi.org/10.15204/jkobgy.2010.23.3.101

Comparative Study of 25 Herbal Formulas on Anti-Inflammatory Effect  

Lee, Jin-Ah (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Ha, Hye-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Jung, Da-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Lee, Ho-Young (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Lee, Jun-Kyung (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Huang, Dae-Sun (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Shin, Hyeun-Kyoo (Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.23, no.3, 2010 , pp. 101-111 More about this Journal
Abstract
Purpose: To provide the information of efficacy for herbal formulas of high frequency, it was evaluated the anti-inflammatory effect. In many studies, plantderived anti-inflammatory efficacies have been investigated for their potential inhibitory effects on lipopolysaccharide (LPS)-stimulated macrophages. This study was performed to examine the anti-inflammatory effects of herbal formulas of high frequency on LPS-stimulated RAW 264.7 cells. Methods: Anti-inflammatory activity was investigated in 25 herbal formula extracts in vitro and in vivo. To investigate the anti-inflammatory effect in vitro model, using LPS-stimulated macrophages, RAW 264.7 cell line. The productions of nitric oxide(NO), prostaglandin(PG)$E_2$, interleukin(IL)-6 and tumor necrosis factor(TNF)-$\alpha$ were examined in RAW 264.7 cells, in the presence of the herbal formulas. RAW 264.7 cells were incubated with LPS $1\;{\mu}g/mL$ and herbal formulas for 18 hours. As an in vivo, using a rat model of carrageenin-induced paw edema. The paw volume was measured at 2 and 4 hours following carrageenin-induced paw edema in rats. Results: 8 kinds of herbal formula inhibited NO production by LPS-stimulated in some concentration, but the effect of NO inhibition is weak. 12 kinds of herbal formula inhibited $PGE_2$ production by LPS-stimulated over the 30%. Among them Gumiganghwal-tang, Sagunja-tang, Samchulkunbi-tang, Insampaedok-san and Hwangryunhaedok-tang inhibited IL-6 production by LPS-stimulated but TNF-$\alpha$ was not inhibited. 12 kinds of herbal formula reduced the carrageenin-induced paw edema in rats. Particularly, 3 kinds of herbal formula(Gumiganghwal-tang, Ssanghwa-tang and Soshiho-tang) were better than indomethacin. Conclusion: These results suggest that Gumiganghwal-tang, Sangunja-tang, Samchulkunbi-tang, Insampaedok-san and Hwangryunhaedok-tang have antiinflammatory activity.
Keywords
Anti-inflammatory; NO; $PGE_2$; Paw edema;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 Mizukawa H et al. The effect of orengedokuto on experimentallyinflamed rats. Am J Chin Med 1993 ;21:71-8.   DOI   ScienceOn
2 Van Snick J. Interleukin-6: an overview. Annu Rev Immunol. 1990;8:253-78.   DOI   ScienceOn
3 Szabo C, Ohshima H. DNA domage induced by peroxynitrite: subsequent biological effects. Nitric Oxide. 1997; 1:373-85.   DOI   ScienceOn
4 Moore RA, Derry S, McQuay HJ. Cyclo-oxygenase-2 selective inhibitors and nonsteroidal anti-inflammatory drugs: balancing gastrointestinal and cardiovascular risk. BMC Musculoskelet Disord. 2007;8:73.   DOI   ScienceOn
5 식품의약품안전청. 한국인의 한약 복용실태 조사 연구. 2006
6 대한병리학회. 간추린 병리학. 서울:정문각. 2002:63-79.
7 Yoon HJ et al. Research Papers: Effects of Chitosamoligosaccharide on the C.albicans-induced inflammatory effect in mice and RAW 264.7 macrophage cells. J Chitin Chitosan. 2007;12(1):15-20.
8 Di Rosa M et al. The mechaism of the inflammatory effect of carrageenin, Eur J Pharmacol., 1968;4:340-2.   DOI   ScienceOn
9 Wei W et al. Antiinflammatory and immuno pharma cology. 1st ed. Beijing:Renmin weisheng chubanshe. 2004:10-17.
10 McCann SM et al. The nitric oxide theory og aging revisited. Annals of the New York Academy of Science 2005;1057:64-84.
11 이진아 등. 사군자탕의 항염증 효능연구. 대한한의학방제학회지. 2010;18(1) :87-94.   과학기술학회마을
12 Moncada S, Palmer RM, Higgs EA. Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol Rev 1991;43:109-42.
13 Wang LM, Mineshita S. Preventive effects of unsei-in and oren-gedoku-to, chinese tranditional medicines, against rat paw oedema and abdominal constriction in mice. J Pharm Pharmacol 1996;48:327-31.   DOI   ScienceOn
14 Wang LM et al. Effects of orengedoku- to and unsei-in, chinese tranditional medicines, on interleukin-8 and superoxide dismutase in rats. J Pharm Pharmacol 1997;49:102-4.   DOI   ScienceOn
15 김일혁, 황규진. 쌍화탕의 항염증작용에 관한 연구. 생약학회지. 1981;28(4):131-5.
16 Masahiko K et al. Effect of Shosaiko- to extract on hepatic inflammation and fibrosis in dimethylnitrosamine induced liver injury rats. Biol Pharm Bull. 2002;25(11):1417-1421.   DOI   ScienceOn
17 이승연, 김장현. 삼출건비탕이 흰쥐의 면역반응에 미치는 영향. 대한한방소아과학회지. 1998;12(1):257-76.
18 이진아 등. 삼출건비탕의 항염증 효능 연구. 대한한의학회지. 2010;31(3):47-54.   과학기술학회마을
19 서창섭 등. 전탕 전과 후의 중금속, 잔류농약 및 잔류이산화황의 농도변화 - 다빈도 한약 처방을 중심으로-. 대한본초학회지. 2009;24(2):13-20.   과학기술학회마을
20 문영희, 고재종, 박조영. 구미강활탕의 소염, 진통작용. 생약학회지. 1999;30(1):18-24.
21 서창섭 등. 전탕 전과 후의 중금속, 잔류농약 및 잔류이산화황의 농도변화 - 소화기계 약을 중심으로-. 대한본초학회지. 2009;24(1):111-9.   과학기술학회마을
22 보건복지부 고시 제1999-23호. 1999. 8. 1
23 서창섭 등. 전탕 전과 후의 중금속, 잔류농약 및 잔류이산화황의 농도변화 - 십전대보탕 구성처방을 중심으로-. 대한한의학회지. 2009;30(4):108-17.   과학기술학회마을
24 Ialenti A et al. Modulation of acute inflammation by endogenous nitrite oxide. Eur J Pharmacol. 1992;211(2) :177-82.   DOI   ScienceOn
25 서창섭 등. 전탕 전과 후의 중금속, 잔류농약 및 잔류이산화황의 농도변화 - 감기약을 중심으로-. 대한본초학회지. 2008;23(4):51-8.   과학기술학회마을
26 Duncan GS et al. The local antiinflammatory action of dexamethasone in the rat carrageenin oedema model is reversed by an antiserum to lipocortin 1. Br J Pharmacol. 1993; 108(1):62-5.   DOI   ScienceOn
27 Wang JP et al. Anti-inflammatory and analgesic effects of magnolol. Naunyn Schmiedebergs Arch Pharmacol. 1992;346(6):707-12.
28 Posadas I et al. Co-regulation between cyclo-oxygenase-2 and inducible nitric oxide synthase expression in the timecourse of murine inflammation. Naunyn Schmiedebergs Arch Pharmacol. 2000; 361(1):98-106.   DOI   ScienceOn
29 Tomlinson A et al. Cyclo-oxygenase and nitric oxide synthase isoforms in rat carrageenin-induced pleurisy. Br J Pharmacol. 1994;113(3):693-8.   DOI   ScienceOn
30 Palmer RM, Eveson JW. Chronic sialadenitis. An immunocytochemical study in humans. Virchows Arch A Pathol Anat Histopathol. 1987;412(1) :73-8.   DOI   ScienceOn
31 보건복지부 고시 제1995-15호. 1995. 3. 15