한국토양비료학회지 (Korean Journal of Soil Science and Fertilizer)

  • 제43권4호
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  • Pages.490-497
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  • 2010
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  • 0367-6315(pISSN)
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  • 2288-2162(eISSN)
한국토양비료학회 (Korean Society of Soil Science and Fertilizer)
권호 표지

Lysobacter enzymogenes LE429와 Neem oil을 이용한 고추 병해의 생물학적 방제

Biocontrol of pepper diseases by Lysobacter enzymogenes LE429 and Neem Oil

  • ;
  • 조민영 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 이용성 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 박윤석 ((주)푸르네) ;
  • 박노동 (전남대학교 농업생명과학대학 응용생물공학부) ;
  • 남이 (농협중앙회 안성교육원) ;
  • 김길용 (전남대학교 농업생명과학대학 응용생물공학부)
  • Han, Thazin (Department of Biotechnology, Technological University) ;
  • Cho, Min-Young (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University) ;
  • Lee, Yong-Seong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University) ;
  • Park, Yun-Seok (Purne Co., Ltd.) ;
  • Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University) ;
  • Nam, Yi (Ansung Training Institute, National Agricultural Cooperative Federation) ;
  • Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
  • 투고 : 2010.07.27
  • 심사 : 2010.08.15
  • 발행 : 2010.08.31
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초록

근권토양으로부터 고추역병균을 포함한 다양한 식물 병원성 곰팡이에 대하여 항균활성이 강한 세균을 분리하였다. 이 세균은 16S rRNA gene서열 분석 결과 Lysobacter enzymogens로 동정되었고 LE429로 명명 하였다. LE429는 chitinase, ${\beta}-1$, 3-glucanase, protease, gelatinase, lipase 및 항생물질과 같은 다양한 이차대사산물을 분비하였다. 항생물질은 diaon HP-20 및 sephadex LH-20 컬럼크로마토그래피 및 HPLC로 정제하여, GC-EI 및 GC-CI분석을 통하여 phenylacetic acid로 동정되었다. Field 실험에서 LE429의 고추 병해 억제 효과를 조사하기 위해 LE429배양액(CB), Neem oil 용액 (NO), LE429배양액과 Neem oil 용액을 섞은 혼합액(CB+NO), 그리고 대조구로서 물(CON)을 각각 고추에 처리하였다. 고추의 수량구성요소는 일반적으로 CB 처리구가 가장 높았고, CB+NO, CON 그리고 NO 순서로 나타났다. CB 처리구에서 병원성 곰팡이는 강하게 억제 되었지만, 몇몇 해충이 발견되었다. NO 처리구에서는 해충은 발견 되지 않았지만, 병원성 곰팡이가 발견 되었다. 하지만, CB+NO 처리구에서 병원성 곰팡이 및 해충이 전혀 발견 되지 않았다. 결론적으로, 2차 대 사산물을 생산하는 LE429와 Neem oil의 혼합액은 고추에 발생하는 병원성 곰팡이와 해충에 대한 좋은 생물학적 방제제가 될 수 있다고 사료된다.

A chitinolytic bacterium having a strong antagonistic activity against various pathogens including Phytophtora capsici was isolated from rhizosphere soil, and identified as Lysobacter enzymogenes (named as LE429) based on 16S rRNA gene sequence analysis. This strain produced a number of substances such as chitinase, ${\beta}-1$, 3-glucanase, lipase, protease, gelatinase and an antibiotic compound. This antibiotic compound was purified by diaion HP-20, sephadex LH-20 column chromatography and HPLC. The purified compound was identified as phenylacetic acid by gas chromatography-electron ionization (GC-EI) and gas chromatography-chemical ionization (GC-CI) mass spectrometry. In field experiment, pepper plants were treated by the strain LE429 culture (CB), neem oil solution (NO), combination (CB+NO) or control (CON). Plant height and number of branches, flowers and pods of pepper plant in CB treatment were generally highest, and followed by CB+NO, CON and NO. The fungal pathogens were strongly inhibited, while several insect pests were discovered in CB treatment. Any insect pests were not found, while all fungal pathogens tested were not suppressed in NO treatment. However, in CB+NO treatment, non incidence of fungal pathogens and insect pests were found. The strain LE429 producing secondary metabolites with neem oil should be a potential agent to control fungal diseases and insect pests.

키워드

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