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Biocontrol of pepper diseases by Lysobacter enzymogenes LE429 and Neem Oil  

Han, Thazin (Department of Biotechnology, Technological University)
Cho, Min-Young (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Lee, Yong-Seong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Park, Yun-Seok (Purne Co., Ltd.)
Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Nam, Yi (Ansung Training Institute, National Agricultural Cooperative Federation)
Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, Chonnam National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.43, no.4, 2010 , pp. 490-497 More about this Journal
Abstract
A chitinolytic bacterium having a strong antagonistic activity against various pathogens including Phytophtora capsici was isolated from rhizosphere soil, and identified as Lysobacter enzymogenes (named as LE429) based on 16S rRNA gene sequence analysis. This strain produced a number of substances such as chitinase, ${\beta}-1$, 3-glucanase, lipase, protease, gelatinase and an antibiotic compound. This antibiotic compound was purified by diaion HP-20, sephadex LH-20 column chromatography and HPLC. The purified compound was identified as phenylacetic acid by gas chromatography-electron ionization (GC-EI) and gas chromatography-chemical ionization (GC-CI) mass spectrometry. In field experiment, pepper plants were treated by the strain LE429 culture (CB), neem oil solution (NO), combination (CB+NO) or control (CON). Plant height and number of branches, flowers and pods of pepper plant in CB treatment were generally highest, and followed by CB+NO, CON and NO. The fungal pathogens were strongly inhibited, while several insect pests were discovered in CB treatment. Any insect pests were not found, while all fungal pathogens tested were not suppressed in NO treatment. However, in CB+NO treatment, non incidence of fungal pathogens and insect pests were found. The strain LE429 producing secondary metabolites with neem oil should be a potential agent to control fungal diseases and insect pests.
Keywords
Lysobacter enzymogenes LE429; Lytic enzyme; Phenylacetic acid; Chitinase; Antifungal activity; Neem oil;
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