Journal of Microbiology and Biotechnology

  • 제20권7호
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  • Pages.1152-1155
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  • 2010
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Establishment of an Axenic Culture of Microcystin-Producing Microcystis aeruginosa Isolated from a Korean Reservoir

  • Han, Ah-Won (Department of Environmental Engineering, Chungbuk National University) ;
  • Oh, Kyoung-Hee (Department of Environmental Engineering, Chungbuk National University) ;
  • Jheong, Weon-Hwa (National Institute of Environmental Research) ;
  • Cho, Young-Cheol (Department of Environmental Engineering, Chungbuk National University)
  • 투고 : 2010.03.17
  • 심사 : 2010.04.03
  • 발행 : 2010.07.28
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초록

In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipenem, were applied separately in that order. Axenicity of the culture was confirmed by cultivation on bacterial media and observation under epifluorescence and scanning electron microscopes. We are the first to establish an axenic culture of a Microcystis strain isolated from Korean reservoirs and can be used in physiological and molecular studies to control toxic Microcystis blooms.

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참고문헌

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피인용 문헌

  1. Simultaneous Quantification of Cyanobacteria and Microcystis spp. Using Real-Time PCR vol.22, pp.2, 2010, https://doi.org/10.4014/jmb.1109.09051
  2. Quantification of toxigenic Microcystis spp. in freshwaters by quantitative real-time PCR based on the microcystin synthetase A gene vol.51, pp.1, 2010, https://doi.org/10.1007/s12275-013-2354-z