Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Biological Activities of Coreopsis tinctoria Nutt. Flower Extracts

기생초 꽃 추출물의 생리활성

  • Hwang, In-Guk (Department of Food Science and Technology, Chungbuk National University) ;
  • Kim, Hyun-Young (Department of Food Science and Technology, Chungbuk National University) ;
  • Shin, So-Lim (Department of Horticultural Science, Chungbuk National University) ;
  • Lee, Cheol-Hee (Department of Horticultural Science, Chungbuk National University) ;
  • Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University) ;
  • Jang, Keum-Il (Department of Food Science and Technology, Chungbuk National University) ;
  • Jeong, Heon-Sang (Department of Food Science and Technology, Chungbuk National University)
  • Received : 2010.03.04
  • Accepted : 2010.06.11
  • Published : 2010.10.31
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Abstract

This study was conducted to evaluate the antioxidant activity, Angiotensin I Converting Enzyme (ACE) inhibitory activity, ${\alpha}$-glucosidase inhibitory activity, nitrate synthesis inhibitory activity, and antiproliferation inhibitory effect on ethanol extract and its solvent fractions of $Coreopsis$ $tinctoria$ Nutt. Ethyl acetate fraction was the strongest at 1,1-diphenyl-2-picryl hydrazyl (DPPH) ($IC_{50}=0.100mg{\cdot}mL^{-1}$) and 2,2'-Azino-bis-(3-ethylbenozothiazoline-6-sulfonic acid) (ABTS) (15.785 mg AA $eq{\cdot}10mg^{-1}$) radical scavenging activity, ACE (40.96% at $1mg{\cdot}mL^{-1}$), and ${\alpha}$-glucosidase ($IC_{50}=0.125mg{\cdot}mL^{-1}$) inhibitory effect among the solvent fractions. Nitrate synthesis inhibitory activity of ethanol extract, chloroform fraction, and ethyl acetate fraction effectively inhibited NO formation in a dose-dependent manner without the cytotoxic effect. Ethanol extract and its solvent fractions inhibited growth of HCT-116 colon cancer cells in a dose-dependent manner. n-Hexane fraction showed the highest antiproliferation inhibitory effect of $0.041mg{\cdot}mL^{-1}$ among fractions.

기생초 꽃의 생리활성을 평가하여 천연물 유래 기능성 소재를 개발하기 위하여 에탄올 추출물 및 용매분획물의 항산화활성, ACE 저해활성, ${\alpha}$-glucosidase 억제능, 항염활성 및 항암활성을 측정하였다. 에탄올 추출물의 DPPH radical 소거 활성($IC_{50}$)과 ABTS radical 소거활성은 각각 $0.100mg{\cdot}mL^{-1}$ 및 3.427 AEAC였으며, 용매분획물 중 ethyl acetate 분획물이 각각 $0.034mg{\cdot}mL^{-1}$ 및 15.785AEAC으로 가장 높았으며, ACE 저해활성 및 ${\alpha}$-glucosidase 억제활성도 각각 40.96% 및 $0.125mg{\cdot}mL^{-1}$로 ethyl acetate 분획물이 우수하였다. 또한 에탄올 추출물, chloroform 및 ethyl acetate 분획물에서 세포독성 없이 효과적으로 NO의 생성을 억제하였고, 대장암 세포주에 대한 에탄올 추출물, n-hexane, chloroform 및 ethyl acetate 분획물의 $IC_{50}$값은 각각 0.208, 0.041, 0.142 및 $0.107mg{\cdot}mL^{-1}$으로 우수한 증식억제효과를 보였다. 이러한 결과를 종합하여 볼 때, 기생초 꽃의 우수한 생리활성을 이용한 기능성 소재로의 활용이 가능 할 것으로 판단된다.

Keywords

References

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