한국가금학회지 (Korean Journal of Poultry Science)

  • 제37권1호
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  • Pages.69-80
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  • 2010
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  • 1225-6625(pISSN)
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  • 2287-5387(eISSN)
한국가금학회 (The Korean Society of Poultry Science)
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대두단백질 및 카제인 섭취가 병아리의 혈액 Cholesterol 함량에 미치는 영향 비교

Comparative Effects of Dietary Isolated Soy Protein and Casein on Plasma Cholesterol Levels in Young Chicks

  • 지규만 (고려대학교 생명과학대학 영양생화학연구실) ;
  • 김영미 (고려대학교 생명과학대학 영양생화학연구실) ;
  • 전지영 (고려대학교 생명과학대학 영양생화학연구실) ;
  • 최인숙 (고려대학교 생명과학대학 영양생화학연구실) ;
  • 오미향 (고려대학교 생명과학대학 영양생화학연구실)
  • Chee, Kew-Mahn (Laboratory of Nutritional Biochemistry, College of Life Sciences and Biotechnology, Korea University) ;
  • Kim, Young-Mee (Laboratory of Nutritional Biochemistry, College of Life Sciences and Biotechnology, Korea University) ;
  • Juhn, Jee-Young (Laboratory of Nutritional Biochemistry, College of Life Sciences and Biotechnology, Korea University) ;
  • Choi, In-Sook (Laboratory of Nutritional Biochemistry, College of Life Sciences and Biotechnology, Korea University) ;
  • Oh, Mi-Hyang (Laboratory of Nutritional Biochemistry, College of Life Sciences and Biotechnology, Korea University)
  • 투고 : 2010.03.02
  • 심사 : 2010.03.14
  • 발행 : 2010.03.31
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초록

대두단백질(ISP)이 혈액 콜레스테롤 농도에 미치는 영향을 확인하기 위하여, 카제인, 어분, 쌀단백질, 옥수수 단백질(글루텐 밀) 등과 함께 이들을 각각 함유한 반정제(semi-purified) 사료를 2주간 병아리에게 급여하였다. 병아리는 쥐에 비해 혈액 콜레스테롤에 예민하게 반응하며, 인체의 지질 실험 모델로 더 적합한 동물이다. 각 단백질 사료 마다 NRC 요구량을 기준하여 부족한 필수아미노산(lysine, arginine 등)을 첨가하였다. 모든 실험 사료는 강제 급이 방식으로 1일 3회 급여하여 아미노산을 제외한 모든 영양소의 섭취량을 동일하게 하였다. 실험 2에서는 모든 사료에 콜레스테롤을 0.3% 수준으로 첨가하여 급여하였다. 카제인군의 병아리 증체량이 두 실험 모두 가장 저조하였고(P<0.05), 대두단백질과 어분군의 성장률이 가장 좋았다. 카제인군은 실험 1, 2에서 혈액 총콜레스테롤, non-HDL 콜레스테롤, TG 함량이 유의하게 가장 높았고, 대두단백질 군은 다른 단백질군(쌀 옥수수단백질, 어분)과 대체로 비슷한 수준을 보였다. 어분군은 실험 2에서 혈액 콜레스테롤이 대두 쌀 옥수수단백질군 보다 유의하게 더 높았다. 카제인군은 간 중량이 두 실험 모두 가장 무거웠고 간의 콜레스테롤 및 총지질 함량 도 가장 많았다. 반면에 어분군은 콜레스테롤 함량을 제외하고 대두 쌀 옥수수단백질 군과 같은 수준을 보였다. 카제인군에서 나타난 고콜레스테롤혈증(hypercholesterolemia)은 전형적인 Lys- Arg 길항 작용에 의한 결과이며, 카제인을 섭취한 대부분의 동물에서 나타나는 현상이다. 반면에 대두단백질과 쌀단백질, 옥수수단백질 및 어분군의 혈중 콜레스테롤 함량이 비슷하게 나타나는 것은 사료 Lys/Arg 비율이 카제인 만큼 심하게 높지 않기 때문이라고 생각된다. 결론적으로 대두단백질군의 저콜레스테롤 혈증(hypocholesterolemia)은 카제인에 비교할 때만 나타나는 상대적인 현상으로, 대두단백질 자체가 혈중 콜레스테롤을 저하시킬 수 있는 독자적인 기능을 갖고 있지 않다고 판단된다.

Hypocholesterolemic effect of soy protein was examined in comparison with casein and three other dietary protein sources in chicks. In two feeding trials, 40 (Expt.1) or 50 (Expt. 2), three-day-old, male chicks were forced-fed each of five semi-purified-type diets containing isolated soy protein (ISP, cp 82%), casein (cp 92%), rice protein (RP, cp 70%), corn gluten meal (CGM, cp 65%) or fish meal (FM, cp 70%) for two weeks. The diets for Expt. 2 were supplemented with 0.3% cholesterol by replacing glucose. Each protein source was the only source of protein of each diet. Essential amino acids were added to the diets to satisfy their requirements according to NRC. The diets were forced-fed to equalize the intake of all nutrients except the amino acids which were inherently variable in the diets. Chicks fed casein showed lower body weight gain than those fed the other proteins in both experiments. Birds fed ISP and FM gained better body weight than the others. Chicks fed casein showed significantly higher levels of plasma total cholesterol, non-HDL cholesterol and triacylglycerol (TG) than those fed ISP and the other protein sources. Meanwhile, the chicks fed ISP, RP, CGM and FM showed comparable levels of plasma total cholesterol, non-HDL cholesterol and TG. In Expt. 2, the birds fed casein and FM showed markedly elevated plasma total cholesterol and non-HDL cholesterol levels. Liver weight and levels of total lipids and cholesterol of chicks fed casein appeared significantly higher than those of the other protein diets, whereas those of the chicks fed ISP, RP, CGM and FM appeared comparable except cholesterol in FM group. In conclusion, only the chicks fed casein diets in both experiments always showed significantly higher levels of plasma cholesterol and TG compared to those fed ISP and the other protein sources. These results support the views that casein, which has unique lysine-arginine ratio, is inherently hyper-cholesterolemic, and ISP is hypocholesterolemic only when compared to casein.

키워드

참고문헌

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