The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Resin Flow, Symptom Development, and Lignin Biosynthesis of Two Pine Species in Response to Wounding and Inoculation with Fusarium circinatum

  • Kim, Ki-Woo (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Lee, In-Jung (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Chang-Soo (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Eom, In-Yong (Department of Forest Sciences, Seoul National University) ;
  • Choi, Joon-Weon (Department of Forest Sciences, Seoul National University) ;
  • Lee, Don-Koo (Department of Forest Sciences, Seoul National University) ;
  • Park, Eun-Woo (Department of Agricultural Biotechnology, Seoul National University)
  • Received : 2010.07.15
  • Accepted : 2010.11.10
  • Published : 2010.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Resin flow, symptom development, and lignin biosynthesis in response to wounding and fungal inoculation were investigated in Pinus rigida and Pinus densiflora. The two-year-old seedling stems were subjected to three types of treatments: (i) wounding without inoculation, (ii) wound-inoculation with a conidial suspension of Fusarium circinatum, and (iii) pre-wounding woundinoculation with the fungus 20 days after the initial wounding. Resin flow from wounding sites was more evident in P. rigida than P. densiflora in all treatments. The wound-inoculation with the fungus induced almost two-fold higher levels of resin flow than the other treatments in both species. The pre-wounding woundinoculation appeared to result in a decrease in pitch canker development in the two pine species. Some reductions in disease severity were observed in the prewounding wound-inoculated P. rigida, showing a mean disease severity of less than 85%, compared with approximately 100% in the wound-inoculated stems. Disease severity was approximately 50% in the woundinoculated P. densiflora, whereas 10% in the pre-wounding wound-inoculated stems. Higher amounts of lignin were found from bark (ca. 40%) than from xylem (ca. 30%). The wound-inoculated bark and the pre-wounding wound-inoculated bark exhibited higher amounts of lignin among the other treatments. These results suggest that the wound-inoculation apparently prompt the increase in resin flow and lignin biosynthesis from the two pine species, and the prior wounding may be involved in decreased disease severity against the further invasion of F. circinatum.

Keywords

References

  1. Barrows-Broaddus, J. and Dwinell, L. D. 1983. Histopathology of Fusarium moniliforme var. subglutinans in four species of southern pines. Phytopathology 73:882-889. https://doi.org/10.1094/Phyto-73-882
  2. Bonello, P., Gordon, T. R. and Storer, A. J. 2001. Systemic induced resistance in Monterey pine. For. Path. 31:99-106. https://doi.org/10.1046/j.1439-0329.2001.00230.x
  3. Correll, J. C., Gordon, T. R., McCain, A. H., Fox, J. W., Koehler, C. S., Wood, D. L. and Schultz, M. E. 1991. Pitch canker disease in California: pathogenicity, distribution, and canker development on Monterey pine (Pinus radiata). Plant Dis. 75:676-682. https://doi.org/10.1094/PD-75-0676
  4. Davis, J. M., Wu, H., Cooke, J. E. K., Reed, J. M., Luce, K. S. and Michler, C. H. 2002. Pathogen challenge, salicylic acid, and jasmonic acid regulate expression of chitinase gene homologs in pine. Mol. Plant Microbe Interact. 15:380-387. https://doi.org/10.1094/MPMI.2002.15.4.380
  5. Esteban, L. G., Gasson, P., Climent, J. M., De Palacios, P. and Guindeo, A. 2005. The wood of Pinus canariensis and its resinous heartwood. IAWA J. 26:69-77. https://doi.org/10.1163/22941932-90001602
  6. Gordon, T. R. 2006. Pitch canker disease of pines. Phytopathology 96:657-659. https://doi.org/10.1094/PHYTO-96-0657
  7. Franceschi, V. R., Krokene, P., Christiansen, E. and Krekling, T. 2005. Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytol. 167:353-376. https://doi.org/10.1111/j.1469-8137.2005.01436.x
  8. Hammerschmidt, R. 2006. Host-pathogen interaction in conifers: Complicated systems yield interesting possibilities for research. Physiol. Mol. Plant Pathol. 68:93-94. https://doi.org/10.1016/j.pmpp.2007.01.002
  9. Hudgins, J. W., Christiansen, E. and Franceschi, V. R. 2004. Induction of anatomically based defense responses in stems of diverse conifers by methyl jasmonate: a phylogenetic perspective. Tree Physiol. 24:251-264. https://doi.org/10.1093/treephys/24.3.251
  10. Kim, K. W., Lee, I. J., Thoungchaleun, V., Kim, C. S., Lee, D. K. and Park, E. W. 2009. Visualization of wound periderm and hyphal profiles in pine stems inoculated with the pitch canker fungus Fusarium circinatum. Microsc. Res. Techniq. 72:965-973. https://doi.org/10.1002/jemt.20744
  11. Kim, Y.-S., Woo, K.-S., Koo, Y.-B. and Yeo, J.-K. 2008b. Variation in susceptibility of six pine species and hybrids to pitch canker caused by Fusarium circinatum. For. Path. 38:419-428. https://doi.org/10.1111/j.1439-0329.2008.00558.x
  12. Knebel, L., Robinson, D. J., Wentworth, T. R. and Klepzig, K. D. 2008. Resin flow responses to fertilization, wounding and fungal inoculation in loblolly pine (Pinus taeda) in North Carolina. Tree Physiol. 28:847-853. https://doi.org/10.1093/treephys/28.6.847
  13. Lee, J. K., Lee, S.-H., Yang, S.-I. and Lee, Y.-W. 2000. First report of pitch canker disease on Pinus rigida in Korea. Plant Pathol. J. 16:52-54.
  14. Lombardero, M. J., Ayres, M. P. and Ayres, B. D. 2006. Effects of fire and mechanical wounding on Pinus resinosa resin defenses, beetle attacks, and pathogens. For. Ecol. Manage. 225:349-358. https://doi.org/10.1016/j.foreco.2006.01.010
  15. Lombardero, M. J., Ayres, M. P., Lorio, P. L. Jr. and Ruel, J. J. 2000. Environmental effects on constitutive and inducible resin defences of Pinus taeda. Ecol. Lett. 3:329-339. https://doi.org/10.1046/j.1461-0248.2000.00163.x
  16. Luchi, N., Ma, R., Capretti, P. and Bonello, P. 2005. Systemic induction of traumatic resin ducts and resin flow in Austrian pine by wounding and inoculation with Sphaeropsis sapinea and Diplodia scrobiculata. Planta 221:75-84. https://doi.org/10.1007/s00425-004-1414-3
  17. Morse, A. M., Nelson, C. D., Covert, S. F., Holliday, A. G., Smith, K. E. and Davis, J. M. 2004. Pine genes regulated by the necrotrophic pathogen Fusarium circinatum. Theor. Appl. Genet. 109:922-932. https://doi.org/10.1007/s00122-004-1719-4
  18. Mumm, R. and Hilker, M. 2006. Direct and indirect chemical defence of pine against folivorous insects. Tren. Plant Sci. 11:351-358. https://doi.org/10.1016/j.tplants.2006.05.007
  19. Nirenberg, H. I. and O’Donnell, K. 1998. New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434-458. https://doi.org/10.2307/3761403
  20. Phillips, M. A. and Croteau, R. B. 1999. Resin-based defenses in conifers. Tren. Plant Sci. 4:184-190. https://doi.org/10.1016/S1360-1385(99)01401-6
  21. Schmale, D. G. and Gordon, T. R. 2003. Variation in susceptibility to pitch canker disease, caused by Fusarium circinatum, in native stands of Pinus muricata. Plant Pathol. 52:720-725. https://doi.org/10.1111/j.1365-3059.2003.00925.x
  22. Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D. and Crocker, D. 2008. Determination of structural carbohydrates and lignin in biomass. In: Laboratory Analytical Procedure (LAP). pp. 1-16. National Renewable Energy Laboratory, Golden, USA.
  23. Thoungchaleun, V., Kim, K. W., Lee, D. K., Kim, C. S. and Park, E. W. 2008. Pre-infection behavior of the pitch canker fungus Fusarium circinatum on pine stems. Plant Pathol. J. 24:112-117. https://doi.org/10.5423/PPJ.2008.24.2.112
  24. Viljoem, A., Wingfield, M. J. and Marasas, W. F. O. 1994. First report of Fusarium subglutinans f. sp. pini on pine seedlings in South Africa. Plant Dis. 78:309-312. https://doi.org/10.1094/PD-78-0309
  25. Wingfield, M. J., Jacobs, A., Coutinho, T. A., Ahumada, R. and Wingfield, B. D. 2002. First report of the pitch canker fungus, Fusarium circinatum, on pines in Chile. Plant Pathol. 51:397. https://doi.org/10.1046/j.1365-3059.2002.00710.x
  26. Woo, K. S., Kim, Y. J., Kim, T. S. and Lee, S. K. 2005. Selection of virulent isolates of Fusarium circinatum and investigation of pitch canker severity of Pinus rigida and P. rigida ${\times}$ P. taeda seed orchards in Jeju island. J. Kor. For. Sci. 94:402-409.

Cited by

  1. Entre sermón oral y comentario escrito: una reflexión sobre la polémica del rabino Joseph Ben Shoshan en su comentario sobre el Avot vol.42, pp.1, 2012, https://doi.org/10.3989/aem.2012.42.1.09
  2. Resin yield in Pinus pinaster is related to tree dendrometry, stand density and tapping-induced systemic changes in xylem anatomy vol.313, 2014, https://doi.org/10.1016/j.foreco.2013.10.038
  3. Adaptive Potential of Maritime Pine (Pinus pinaster) Populations to the Emerging Pitch Canker Pathogen, Fusarium circinatum vol.9, pp.12, 2014, https://doi.org/10.1371/journal.pone.0114971
  4. Screening of Maritime pine (Pinus pinaster) for resistance to Fusarium circinatum, the causal agent of Pitch Canker disease vol.85, pp.2, 2012, https://doi.org/10.1093/forestry/cpr055
  5. Effect of four tapping methods on anatomical traits and resin yield in Maritime pine (Pinus pinaster Ait.) vol.86, 2016, https://doi.org/10.1016/j.indcrop.2016.03.033
  6. Spatial and temporal dynamics of the colonization ofPinus radiatabyFusarium circinatum, of conidiophora development in the pith and of traumatic resin duct formation vol.198, pp.4, 2013, https://doi.org/10.1111/nph.12222