Browse > Article
http://dx.doi.org/10.5423/PPJ.2010.26.4.394

Resin Flow, Symptom Development, and Lignin Biosynthesis of Two Pine Species in Response to Wounding and Inoculation with Fusarium circinatum  

Kim, Ki-Woo (National Instrumentation Center for Environmental Management, Seoul National University)
Lee, In-Jung (Research Institute for Agriculture and Life Sciences, Seoul National University)
Kim, Chang-Soo (Department of Forest Genetic Resources, Korea Forest Research Institute)
Eom, In-Yong (Department of Forest Sciences, Seoul National University)
Choi, Joon-Weon (Department of Forest Sciences, Seoul National University)
Lee, Don-Koo (Department of Forest Sciences, Seoul National University)
Park, Eun-Woo (Department of Agricultural Biotechnology, Seoul National University)
Publication Information
The Plant Pathology Journal / v.26, no.4, 2010 , pp. 394-401 More about this Journal
Abstract
Resin flow, symptom development, and lignin biosynthesis in response to wounding and fungal inoculation were investigated in Pinus rigida and Pinus densiflora. The two-year-old seedling stems were subjected to three types of treatments: (i) wounding without inoculation, (ii) wound-inoculation with a conidial suspension of Fusarium circinatum, and (iii) pre-wounding woundinoculation with the fungus 20 days after the initial wounding. Resin flow from wounding sites was more evident in P. rigida than P. densiflora in all treatments. The wound-inoculation with the fungus induced almost two-fold higher levels of resin flow than the other treatments in both species. The pre-wounding woundinoculation appeared to result in a decrease in pitch canker development in the two pine species. Some reductions in disease severity were observed in the prewounding wound-inoculated P. rigida, showing a mean disease severity of less than 85%, compared with approximately 100% in the wound-inoculated stems. Disease severity was approximately 50% in the woundinoculated P. densiflora, whereas 10% in the pre-wounding wound-inoculated stems. Higher amounts of lignin were found from bark (ca. 40%) than from xylem (ca. 30%). The wound-inoculated bark and the pre-wounding wound-inoculated bark exhibited higher amounts of lignin among the other treatments. These results suggest that the wound-inoculation apparently prompt the increase in resin flow and lignin biosynthesis from the two pine species, and the prior wounding may be involved in decreased disease severity against the further invasion of F. circinatum.
Keywords
Fusarium circinatum; inoculation; lignin; pitch canker; resin; wound;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
1 Morse, A. M., Nelson, C. D., Covert, S. F., Holliday, A. G., Smith, K. E. and Davis, J. M. 2004. Pine genes regulated by the necrotrophic pathogen Fusarium circinatum. Theor. Appl. Genet. 109:922-932.   DOI
2 Mumm, R. and Hilker, M. 2006. Direct and indirect chemical defence of pine against folivorous insects. Tren. Plant Sci. 11:351-358.   DOI   ScienceOn
3 Nirenberg, H. I. and O’Donnell, K. 1998. New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90:434-458.   DOI
4 Phillips, M. A. and Croteau, R. B. 1999. Resin-based defenses in conifers. Tren. Plant Sci. 4:184-190.   DOI   ScienceOn
5 Schmale, D. G. and Gordon, T. R. 2003. Variation in susceptibility to pitch canker disease, caused by Fusarium circinatum, in native stands of Pinus muricata. Plant Pathol. 52:720-725.   DOI   ScienceOn
6 Davis, J. M., Wu, H., Cooke, J. E. K., Reed, J. M., Luce, K. S. and Michler, C. H. 2002. Pathogen challenge, salicylic acid, and jasmonic acid regulate expression of chitinase gene homologs in pine. Mol. Plant Microbe Interact. 15:380-387.   DOI   ScienceOn
7 Esteban, L. G., Gasson, P., Climent, J. M., De Palacios, P. and Guindeo, A. 2005. The wood of Pinus canariensis and its resinous heartwood. IAWA J. 26:69-77.   DOI
8 Gordon, T. R. 2006. Pitch canker disease of pines. Phytopathology 96:657-659.   DOI   ScienceOn
9 Franceschi, V. R., Krokene, P., Christiansen, E. and Krekling, T. 2005. Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytol. 167:353-376.   DOI   ScienceOn
10 Hammerschmidt, R. 2006. Host-pathogen interaction in conifers: Complicated systems yield interesting possibilities for research. Physiol. Mol. Plant Pathol. 68:93-94.   DOI   ScienceOn
11 Hudgins, J. W., Christiansen, E. and Franceschi, V. R. 2004. Induction of anatomically based defense responses in stems of diverse conifers by methyl jasmonate: a phylogenetic perspective. Tree Physiol. 24:251-264.   DOI   ScienceOn
12 Kim, K. W., Lee, I. J., Thoungchaleun, V., Kim, C. S., Lee, D. K. and Park, E. W. 2009. Visualization of wound periderm and hyphal profiles in pine stems inoculated with the pitch canker fungus Fusarium circinatum. Microsc. Res. Techniq. 72:965-973.   DOI   ScienceOn
13 Kim, Y.-S., Woo, K.-S., Koo, Y.-B. and Yeo, J.-K. 2008b. Variation in susceptibility of six pine species and hybrids to pitch canker caused by Fusarium circinatum. For. Path. 38:419-428.   DOI   ScienceOn
14 Knebel, L., Robinson, D. J., Wentworth, T. R. and Klepzig, K. D. 2008. Resin flow responses to fertilization, wounding and fungal inoculation in loblolly pine (Pinus taeda) in North Carolina. Tree Physiol. 28:847-853.   DOI   ScienceOn
15 Viljoem, A., Wingfield, M. J. and Marasas, W. F. O. 1994. First report of Fusarium subglutinans f. sp. pini on pine seedlings in South Africa. Plant Dis. 78:309-312.   DOI   ScienceOn
16 Barrows-Broaddus, J. and Dwinell, L. D. 1983. Histopathology of Fusarium moniliforme var. subglutinans in four species of southern pines. Phytopathology 73:882-889.   DOI
17 Bonello, P., Gordon, T. R. and Storer, A. J. 2001. Systemic induced resistance in Monterey pine. For. Path. 31:99-106.   DOI   ScienceOn
18 Correll, J. C., Gordon, T. R., McCain, A. H., Fox, J. W., Koehler, C. S., Wood, D. L. and Schultz, M. E. 1991. Pitch canker disease in California: pathogenicity, distribution, and canker development on Monterey pine (Pinus radiata). Plant Dis. 75:676-682.   DOI
19 Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D. and Crocker, D. 2008. Determination of structural carbohydrates and lignin in biomass. In: Laboratory Analytical Procedure (LAP). pp. 1-16. National Renewable Energy Laboratory, Golden, USA.
20 Thoungchaleun, V., Kim, K. W., Lee, D. K., Kim, C. S. and Park, E. W. 2008. Pre-infection behavior of the pitch canker fungus Fusarium circinatum on pine stems. Plant Pathol. J. 24:112-117.   DOI   ScienceOn
21 Wingfield, M. J., Jacobs, A., Coutinho, T. A., Ahumada, R. and Wingfield, B. D. 2002. First report of the pitch canker fungus, Fusarium circinatum, on pines in Chile. Plant Pathol. 51:397.   DOI   ScienceOn
22 Woo, K. S., Kim, Y. J., Kim, T. S. and Lee, S. K. 2005. Selection of virulent isolates of Fusarium circinatum and investigation of pitch canker severity of Pinus rigida and P. rigida ${\times}$ P. taeda seed orchards in Jeju island. J. Kor. For. Sci. 94:402-409.   과학기술학회마을
23 Luchi, N., Ma, R., Capretti, P. and Bonello, P. 2005. Systemic induction of traumatic resin ducts and resin flow in Austrian pine by wounding and inoculation with Sphaeropsis sapinea and Diplodia scrobiculata. Planta 221:75-84.   DOI
24 Lee, J. K., Lee, S.-H., Yang, S.-I. and Lee, Y.-W. 2000. First report of pitch canker disease on Pinus rigida in Korea. Plant Pathol. J. 16:52-54.   과학기술학회마을
25 Lombardero, M. J., Ayres, M. P. and Ayres, B. D. 2006. Effects of fire and mechanical wounding on Pinus resinosa resin defenses, beetle attacks, and pathogens. For. Ecol. Manage. 225:349-358.   DOI   ScienceOn
26 Lombardero, M. J., Ayres, M. P., Lorio, P. L. Jr. and Ruel, J. J. 2000. Environmental effects on constitutive and inducible resin defences of Pinus taeda. Ecol. Lett. 3:329-339.   DOI   ScienceOn