The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Cloning and Characterization of Soybean Cinnamoyl CoA Reductase Induced by Abiotic Stresses

  • So, Hyun-Ah (BK21 Center for Silver-Bio Industrialization, Dong-A University) ;
  • Chung, Eun-Sook (BK21 Center for Silver-Bio Industrialization, Dong-A University) ;
  • Cho, Chang-Woo (BK21 Center for Silver-Bio Industrialization, Dong-A University) ;
  • Kim, Kee-Young (National Academy of Agricultural Science, RDA) ;
  • Lee, Jai-Heon (BK21 Center for Silver-Bio Industrialization, Dong-A University)
  • Received : 2010.08.10
  • Accepted : 2010.10.26
  • Published : 2010.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Suppression subtractive hybridization was used to isolate wound-induced genes from soybean. One of the wound-induced genes, gmwi143 designated as GmCCR, showed high homology with genes encoding cinnamoyl-CoA reductase (CCR; EC 1.2.1.44). Deduced amino acid sequences encoded by GmCCR showed the highest identity (77%) with those of Acacia CCR. There are 2 CCR genes highly homologous to GmCCR in soybean genome based on Phytozome DB analysis. RNA expression of GmCCR was specifically induced by local and systemic wounding, drought, high salinity or by ultraviolet stress. Our study suggests that GmCCR may be involved in resistance mechanism during abiotic stresses in plants.

Keywords

References

  1. Boerjan, W., Ralph, J. and Baucher, M. 2003. Lignin biosynthesis. Annu. Rev. Plant Biol. 54:519-546. https://doi.org/10.1146/annurev.arplant.54.031902.134938
  2. Chung, E., Cho, C.-W., Kim, K.-Y., Chung, J., Kim, J.-I., Chung, Y.-S., Fukui, K. and Lee, J.-H. 2007. Molecular characterization of the GmAMS1 gene encoding $\beta$-amyrin synthase in soybean plants. Russ. J. Plant Physiol. 54:518-523. https://doi.org/10.1134/S1021443707040139
  3. Church, G. M. and Gilbert, W. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81:1991-1995. https://doi.org/10.1073/pnas.81.7.1991
  4. Costa, M., Collins, R. E., Anterola, A. M., Cochrane, F. C., Davin, L. B. and Lewis, N. G. 2003. An in silico assessment of gene function and organization of the phenylpropanoid pathway metabolic networks in Arabidopsis thaliana and limitations thereof. Phytochemistry 64:1097-1112. https://doi.org/10.1016/S0031-9422(03)00517-X
  5. Jones, L., Ennos, A. R. and Turner, S. R. 2001. Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis. Plant J. 26:205-216. https://doi.org/10.1046/j.1365-313x.2001.01021.x
  6. Kawasaki, T., Koita, H., Nakatsubo, T., Hasegawa, K., Wakabayashi, K., Takahashi, H., Umemura, K., Umezawa, T. and Shimamoto, K. 2006. Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice. Proc. Natl. Acad. Sci. USA 103:230-235. https://doi.org/10.1073/pnas.0509875103
  7. Kim, C. Y., Lee, S. H., Park, H. C., Bae, C. G., Cheong, Y. H., Choi, Y. J., Han, C., Lee, S. Y., Lim, C. O. and Cho, M. J. 2000. Mol. Plant-Microbe Interact. 13:470-474. https://doi.org/10.1094/MPMI.2000.13.4.470
  8. Klink, V. P., Hosseini, P., Matsye, P., Alkharouf, N. W. and Matthews, B. F. 2009. A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) gneotype PI 548402 (Peking) undergoing a resistant after infection by Heterodera glycines (soybean cyst nematode). Plant Mol. Biol. 71:525-567. https://doi.org/10.1007/s11103-009-9539-1
  9. Lacombe, E., Hawkins, S., Van Doorsselaere, J., Piquemal, J., Goffner, D., Poeydomenge, O., Boudet, A.-M. and Grima-Pettenati, J. 1997. Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: cloning, expression and phylogenetic relationships. Plant J. 11:429-441. https://doi.org/10.1046/j.1365-313X.1997.11030429.x
  10. Lacombe, E., Van Doorsselaere, J., Piquemal, J., Boerjan, W. O., Boudet, A. M. and Grima-Pettenati, J. 2000. Characterization of cis-elements required for vascular expression of the cinnamoyl CoA reductase gene and for protein-DNA complex formation. Plant J. 23:663-676. https://doi.org/10.1046/j.1365-313x.2000.00838.x
  11. Lauvergeat, V., Lacomme, C., Lacombe, E., Lasserre, E., Roby, D. and Grima-Pettenati, J. 2001. Two cinnamoyl-CoA reductase (CCR) genes from Arabidopsis thaliana are differentially expressed during development and in response to infection with pathogenic bacteria. Phytochemistry 57:1187-1195. https://doi.org/10.1016/S0031-9422(01)00053-X
  12. Lewis, N. G. and Davin, L. B. 1994. Evolution of lignan and neolignan biochemical pathways. In: Nes D (Ed.), Evolution of Natural Products. ACS Symposium Series American Chemical Society, Washington DC. 562:202-246. https://doi.org/10.1021/bk-1994-0562.ch010
  13. Piquemal, J., Lapierre, C., Myton, K., O’Connell, A., Schuch, W., Gima-Pettenati, J. and Boudet, A. M. 1998. Downregulation of cinnamoylCoA reductase induces significant changes of lignin profiles in transgenic tobacco plants. Plant J. 13:71-83. https://doi.org/10.1046/j.1365-313X.1998.00014.x
  14. Rozema, J., van de Staaij, J., Björn, L. O. and Caldwell, M. 1997. UV-B as an environmental factor in plant life: stress and regulation. Tree 12:22-28.
  15. Shure, M., Wessler, S. and Fedoroff, N. 1983. Molecular identification and isolation of Waxy locus in maize. Cell 35:225-233. https://doi.org/10.1016/0092-8674(83)90225-8
  16. Vance, C. P., Kirk, T. K. and Sherwood, R. T. 1980. Lignification as a mechanism of disease resistance. Annu. Rev. Phytopathol. 18:259-288. https://doi.org/10.1146/annurev.py.18.090180.001355
  17. Van der Rest, B., Danoun, S., Boudet, A.-M. and Rochange, S. F. 2006. Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic pools. J. Exp. Bot. 57:1399-1411. https://doi.org/10.1093/jxb/erj120
  18. Yamasaki, S., Noguchi, N. and Mimaki, K. 2007. Continuous UVB irradiation induces morphological changes and the accumulation of polyphenolic compounds on the surface of cucumber cotyledons. J. Radiat. Res. 48:443-454. https://doi.org/10.1269/jrr.07046

Cited by

  1. Characterization of Developmental- and Stress-Mediated Expression of Cinnamoyl-CoA Reductase in Kenaf (Hibiscus cannabinusL.) vol.2014, 2014, https://doi.org/10.1155/2014/601845
  2. Transcriptomic changes following the compatible interaction Vitis vinifera–Erysiphe necator. Paving the way towards an enantioselective role in plant defence modulation vol.68, 2013, https://doi.org/10.1016/j.plaphy.2013.03.024
  3. Identification of early response genes to salt stress in roots of melon (Cucumis melo L.) seedlings vol.40, pp.4, 2013, https://doi.org/10.1007/s11033-012-2307-3
  4. Differential proteomic analysis revealing the ovule abortion in the female-sterile line of Pinus tabulaeformis Carr. vol.260, 2017, https://doi.org/10.1016/j.plantsci.2017.03.001