Browse > Article
http://dx.doi.org/10.5423/PPJ.2010.26.4.380

Molecular Cloning and Characterization of Soybean Cinnamoyl CoA Reductase Induced by Abiotic Stresses  

So, Hyun-Ah (BK21 Center for Silver-Bio Industrialization, Dong-A University)
Chung, Eun-Sook (BK21 Center for Silver-Bio Industrialization, Dong-A University)
Cho, Chang-Woo (BK21 Center for Silver-Bio Industrialization, Dong-A University)
Kim, Kee-Young (National Academy of Agricultural Science, RDA)
Lee, Jai-Heon (BK21 Center for Silver-Bio Industrialization, Dong-A University)
Publication Information
The Plant Pathology Journal / v.26, no.4, 2010 , pp. 380-385 More about this Journal
Abstract
Suppression subtractive hybridization was used to isolate wound-induced genes from soybean. One of the wound-induced genes, gmwi143 designated as GmCCR, showed high homology with genes encoding cinnamoyl-CoA reductase (CCR; EC 1.2.1.44). Deduced amino acid sequences encoded by GmCCR showed the highest identity (77%) with those of Acacia CCR. There are 2 CCR genes highly homologous to GmCCR in soybean genome based on Phytozome DB analysis. RNA expression of GmCCR was specifically induced by local and systemic wounding, drought, high salinity or by ultraviolet stress. Our study suggests that GmCCR may be involved in resistance mechanism during abiotic stresses in plants.
Keywords
cinnamoyl-coA reductase; lignin; soybean; wounding; UV;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Yamasaki, S., Noguchi, N. and Mimaki, K. 2007. Continuous UVB irradiation induces morphological changes and the accumulation of polyphenolic compounds on the surface of cucumber cotyledons. J. Radiat. Res. 48:443-454.   DOI   ScienceOn
2 Lacombe, E., Van Doorsselaere, J., Piquemal, J., Boerjan, W. O., Boudet, A. M. and Grima-Pettenati, J. 2000. Characterization of cis-elements required for vascular expression of the cinnamoyl CoA reductase gene and for protein-DNA complex formation. Plant J. 23:663-676.   DOI   ScienceOn
3 Rozema, J., van de Staaij, J., Björn, L. O. and Caldwell, M. 1997. UV-B as an environmental factor in plant life: stress and regulation. Tree 12:22-28.
4 Lauvergeat, V., Lacomme, C., Lacombe, E., Lasserre, E., Roby, D. and Grima-Pettenati, J. 2001. Two cinnamoyl-CoA reductase (CCR) genes from Arabidopsis thaliana are differentially expressed during development and in response to infection with pathogenic bacteria. Phytochemistry 57:1187-1195.   DOI   ScienceOn
5 Lewis, N. G. and Davin, L. B. 1994. Evolution of lignan and neolignan biochemical pathways. In: Nes D (Ed.), Evolution of Natural Products. ACS Symposium Series American Chemical Society, Washington DC. 562:202-246.   DOI
6 Piquemal, J., Lapierre, C., Myton, K., O’Connell, A., Schuch, W., Gima-Pettenati, J. and Boudet, A. M. 1998. Downregulation of cinnamoylCoA reductase induces significant changes of lignin profiles in transgenic tobacco plants. Plant J. 13:71-83.   DOI   ScienceOn
7 Shure, M., Wessler, S. and Fedoroff, N. 1983. Molecular identification and isolation of Waxy locus in maize. Cell 35:225-233.   DOI   ScienceOn
8 Vance, C. P., Kirk, T. K. and Sherwood, R. T. 1980. Lignification as a mechanism of disease resistance. Annu. Rev. Phytopathol. 18:259-288.   DOI   ScienceOn
9 Van der Rest, B., Danoun, S., Boudet, A.-M. and Rochange, S. F. 2006. Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic pools. J. Exp. Bot. 57:1399-1411.   DOI   ScienceOn
10 Chung, E., Cho, C.-W., Kim, K.-Y., Chung, J., Kim, J.-I., Chung, Y.-S., Fukui, K. and Lee, J.-H. 2007. Molecular characterization of the GmAMS1 gene encoding $\beta$-amyrin synthase in soybean plants. Russ. J. Plant Physiol. 54:518-523.   DOI
11 Church, G. M. and Gilbert, W. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81:1991-1995.   DOI
12 Costa, M., Collins, R. E., Anterola, A. M., Cochrane, F. C., Davin, L. B. and Lewis, N. G. 2003. An in silico assessment of gene function and organization of the phenylpropanoid pathway metabolic networks in Arabidopsis thaliana and limitations thereof. Phytochemistry 64:1097-1112.   DOI   ScienceOn
13 Jones, L., Ennos, A. R. and Turner, S. R. 2001. Cloning and characterization of irregular xylem4 (irx4): a severely lignin-deficient mutant of Arabidopsis. Plant J. 26:205-216.   DOI   ScienceOn
14 Kawasaki, T., Koita, H., Nakatsubo, T., Hasegawa, K., Wakabayashi, K., Takahashi, H., Umemura, K., Umezawa, T. and Shimamoto, K. 2006. Cinnamoyl-CoA reductase, a key enzyme in lignin biosynthesis, is an effector of small GTPase Rac in defense signaling in rice. Proc. Natl. Acad. Sci. USA 103:230-235.   DOI   ScienceOn
15 Kim, C. Y., Lee, S. H., Park, H. C., Bae, C. G., Cheong, Y. H., Choi, Y. J., Han, C., Lee, S. Y., Lim, C. O. and Cho, M. J. 2000. Mol. Plant-Microbe Interact. 13:470-474.   DOI   ScienceOn
16 Klink, V. P., Hosseini, P., Matsye, P., Alkharouf, N. W. and Matthews, B. F. 2009. A gene expression analysis of syncytia laser microdissected from the roots of the Glycine max (soybean) gneotype PI 548402 (Peking) undergoing a resistant after infection by Heterodera glycines (soybean cyst nematode). Plant Mol. Biol. 71:525-567.   DOI
17 Boerjan, W., Ralph, J. and Baucher, M. 2003. Lignin biosynthesis. Annu. Rev. Plant Biol. 54:519-546.   DOI
18 Lacombe, E., Hawkins, S., Van Doorsselaere, J., Piquemal, J., Goffner, D., Poeydomenge, O., Boudet, A.-M. and Grima-Pettenati, J. 1997. Cinnamoyl CoA reductase, the first committed enzyme of the lignin branch biosynthetic pathway: cloning, expression and phylogenetic relationships. Plant J. 11:429-441.   DOI   ScienceOn