KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characteristics of Polyhydroxyalkanoates Synthesis by Ralstonia eutropha from Vegetable Oils

식물성 오일로부터 Ralstonia eutropha의 polyhydroxyalkanoates 합성 특성

  • Park, Dae-Hoo (Department of Chemical Engineering and Research Institute of Industrial Science and Technology, College of Engineering, Chungbuk National University) ;
  • Kim, Beom-Soo (Department of Chemical Engineering and Research Institute of Industrial Science and Technology, College of Engineering, Chungbuk National University)
  • 박대후 (충북대학교 공과대학 화학공학과 산업과학기술연구소) ;
  • 김범수 (충북대학교 공과대학 화학공학과 산업과학기술연구소)
  • Received : 2009.12.02
  • Accepted : 2010.05.27
  • Published : 2010.06.30
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Abstract

Six strains of Ralstonia eutropha were grown to investigate characteristics of polyhydroxyalkanoates (PHA) synthesis from vegetable oils or glycerol. Poly(3-hydroxybutyrate) homopolymer was formed using soybean oil, olive oil, or glycerol as carbon source, while poly(3-hydroxybutyrate-co-4-hydroxybutyrate) or poly(3-hydroxybutyrateco-3-hydroxyvalerate) copolymers were synthesized by co-feeding $\gamma$-butyrolactone or pentanoic acid, respectively. Optimum strain was determined as R. eutropha KCTC 2662 in terms of final cell concentration and PHA content. From 20 g/L of soybean oil (optimum substrate), cell concentration and PHA content at 72 h ranged 1.7~9.2 g/L and 70~92 wt%, respectively.

식물성 오일 또는 glycerol로부터 Ralstonia eutropha 여섯 균주의 polyhydroxyalkanoates (PHA) 합성 특성을 조사하였다. 탄소원으로 soybean oil, olive oil, 또는 glycerol 만을 공급시 poly(3-hydroxybutyrate) homopolymer가 생성되었으며, $\gamma$-butyrolactone 또는 pentanoic acid를 함께 공급시 poly(3-hydroxybutyrate-co-4-hydroxybutyrate) 또는 poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer가 각각 합성되었다. 최종 균체농도 및 PHA 함량면에서 최적인 균주는 R. eutropha KCTC 2662로 결정되었으며, 최적 기질인 soybean oil 20 g/L로부터 72 h에 균체농도 1.7~9.2 g/L, PHA 함량 70~92 wt%를 얻을 수 있었다.

Keywords

References

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