Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cadaverine is Transported into Vibrio vulnificus Through its CadB in Alkaline Environment

  • Kang, In-Hye (Department of Life Science and Basic Science Institute for Cell Damage Control, Sogang University) ;
  • Kim, Eui-Jin (Department of Life Science and Basic Science Institute for Cell Damage Control, Sogang University) ;
  • Lee, Jeong-K. (Department of Life Science and Basic Science Institute for Cell Damage Control, Sogang University)
  • Published : 2009.10.31
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Abstract

The exogenously added cadaverine is effective in protecting Vibrio vulnificus from methyl viologen (MV)-induced superoxide stress at pH 8.5. Such a protective effect by cadaverine was not observed at pH 7.5. Consistently, the accumulated level of intracellular cadaverine at pH 8.5 is approximately four times as much as that of the control cell at pH 7.5. Cadaverine accumulation is not affected by MV. The protection of V. vulnificus by cadaverine from superoxide stress was abolished when cadB coding for the lysine-cadaverine antiporter was interrupted. However, the cadaverine-mediated protection was complemented with cadB DNA. Therefore, CadB of V. vulnificus not only acts as a lysine-cadaverine antiporter at acid pH to neutralize the external medium, but also mediates cadaverine uptake at alkaline pH to result in cell protection from superoxide stress.

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References

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