Mycobiology

The Korean Society of Mycology (한국균학회)
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Isolation of Genes Specifically Expressed in Different Developmental Stages of Pleurotus ostreatus Using Macroarray Analysis

  • Lee, Seung-Ho (Bio-crops Development Division, National Academy of Agricultural Science, RDA) ;
  • Joh, Joong-Ho (Department of Applied Biochemistry, College of Natural Science, Konkuk University) ;
  • Lee, Jin-Sung (Research Institute of Narae Bioteh, Naraebio Tech Inc.) ;
  • Lim, Jong-Hyun (Department of Applied Biochemistry, College of Natural Science, Konkuk University) ;
  • Kim, Kyung-Yun (Department of Applied Biochemistry, College of Natural Science, Konkuk University) ;
  • Yoo, Young-Bok (Mushroom Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Chang-Soo (Department of Applied Biochemistry, College of Natural Science, Konkuk University) ;
  • Kim, Beom-Gi (Bio-crops Development Division, National Academy of Agricultural Science, RDA)
  • Published : 2009.09.30
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Abstract

The oyster mushroom (Pleurotus ostreatus) is one of the most important edible mushrooms worldwide. The mechanism of P. ostreatus fruiting body development has been of interest both for the basic understanding of the phenotypic change of the mycelium-fruiting body and to improve breeding of the mushrooms. Based on our previous publication of P. ostreatus expressed sequence tag database, 1,528 unigene clones were used in macroarray analysis of mycelium, fruiting body and basidiospore developmental stages of P. ostreatus. Gene expression profile databases generated by evaluating expression levels showed that 33, 10, and 94 genes were abundantly expressed in mycelium, fruiting body and basidiospore developmental stages, respectively. Among them, the genes specifically expressed in the fruiting body stage were further analyzed by reverse transcription-polymerase chain reaction and Northern blot to investigate temporal and spatial expression patterns. These results provide useful information for future studies of edible mushroom development.

Keywords

References

  1. Casselton, L. A. and Olesnicky, N. S. 1998. Molecular genetics of mating recognition in basidiomycete fungi. Microbiol. Mol. Biol. Rev. 62:55-70
  2. Cohen, R., Persky, L. and Hadar, Y. 2002. Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus. Appl. Microbiol. Biotechnol. 58:582-594 https://doi.org/10.1007/s00253-002-0930-y
  3. Dons, J. J., Springer, J., de Vries, S. C. and Wessels, J. G 1984. Molecular cloning of a gene abundantly expressed during fruiting body initiation in Schizophyllum commune. J. Bacteriol. 157:802-808
  4. Fernandez Espinar, M. T. and Labarere, J. 1997. Cloning and sequencing of the Aa-Pril gene specifically expressed during fruiting initiation in the edible mushroom Agrocybe aegerita, and analysis of the predicted amino-acid sequence. Curr. Genet. 32:420-424 https://doi.org/10.1007/s002940050297
  5. Fowler, T. J., Mitton, M. F., Rees, E. I. and Raper, C. A. 2004. Crossing the boundary between the Balpha and Bbeta matingtype loci in Schizophyllum commune. Fungal Genet. Biol. 41:89-101 https://doi.org/10.1016/j.fgb.2003.08.009
  6. Joh, J. H., Lee, S. H., Lee, J. S., Kim, K. H., Jeong, S. J., Youn, W. H., Kim, N. K., Son, E. S., Cho, Y. S., Yoo, Y. B., et al. 2007. Isolation of genes expressed during the developmental stages of the oyster mushroom, Pleurotus ostreatus, using expressed sequence tags. FEMS Microbiol. Lett. 276:19-25 https://doi.org/10.1111/j.1574-6968.2007.00879.x
  7. Kamada, T. 2002. Molecular genetics of sexual development in the mushroom Coprinus cinereus. Bioessays 24:449-459 https://doi.org/10.1002/bies.10083
  8. Kim, B. G, Magae, Y., Yoo, Y, B. and Kwon, S. T. 1999. Iso1ation and transformation of uracil auxotrophs of the edible basidiomycete Pleurotus ostreatus. FEMS Microbiol. Lett. 181:225-22S https://doi.org/10.1111/j.1574-6968.1999.tb08848.x
  9. Kim, B.-G, Joh, J. H., Yoo, Y. B. and Magae, Y. 2003. Transformation of the edible basidiomycetes, PIeurotus ostreatus to phleomycin resistance. Mycobiology 31:42-45 https://doi.org/10.4489/MYCO.2003.31.1.042
  10. Kues, U. and Liu, Y. 2000. Fruiting body production in Basidiomycetes. Appl. Microbiol. Biotechnol. 54:141-152 https://doi.org/10.1007/s002530000396
  11. Kues, U. 2000. Life history and developmental processes in the basidiomycete Coprinus cinereus. Microbiol. Mol. Biol. Rev. 64:316-353 https://doi.org/10.1128/MMBR.64.2.316-353.2000
  12. Kuo, C. Y., Chou, S. Y. and Huang, C. T. 2004. Cloning Of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transformation in Flammulina velutipes. Appl. Microbiol. Biotechnol. 65:593-599
  13. Lee, S. H., Kim, B. G, Kim, K. J., Lee, J. S., Yun, D. W., Hahn, J. H., Kim, G. H., Lee, K. H., Suh, D. S., Kwon, S. T., et al. 2002. Comparative analysis of sequences expressed during the liquid-cultured mycelia and fruit body stages of Pleurotus ostreatus. Fungal Genet. Biol. 35:115-134 https://doi.org/10.1006/fgbi.2001.1310
  14. Leuthner, B., Aichinger, C., Oehmen, E., Koopmann, E., Muller, O, Muller, P., Kahmann, R., Bolker, M. and Schreier, P. H. 2005. A $H_2O_2$-producing glyoxal oxidase is required for filamentous growth and pathogenicity in Ustilago maydis. Mol Genet. Genomics. 272:639-650 https://doi.org/10.1007/s00438-004-1085-6
  15. Liu, Y., Srivilai, R, Loos, S., Aebi, M. and Kues, U. 2006. An essential gene for fruiting body initiation in the basidiomycet Coprinopsis cinerea is homologous to bacterial cyclopropane fatty acid svnthase genes. Genetics 172:873-884 https://doi.org/10.1534/genetics.105.045542
  16. Murguchi, H. and Kamada, T. 1998. The ichl gene of the mushroom Coprinus cinereus is essential for pileus formation in fruiting. Development 125:3133-3141
  17. Sunagawa, M. and Magae, Y. 2005. Isolation of genes ditferentially expressed during the fruit body development of Pleurotus ostreatus by differential display of RAPD. FEMS Microhiotl. Lett. 246:279-284 https://doi.org/10.1016/j.femsle.2005.04.018
  18. Terashima, K., Yuki, K., Muraguchi, H., Akiyama, M. and Kamada, T. 2005. The dstl gene involved in mushroom Photomorphogenesis of Coprinus cinereus encodes a putative Photoreceptor for blue light. Genetics 171:101-108 https://doi.org/10.1534/genetics.104.040048
  19. Thomason, P. A., Sawai, S., Stock, J. B. and Cox, E. C. 2006. The histidine kinase homologue DhkK/Sombrero controls morphogenesis in Dictyostelium. Dev. Biol. 292:358-370 https://doi.org/10.1016/j.ydbio.2006.01.010
  20. Wessels, J. G. 1993. Fruiting in the higher fungi. Adv. Microb. Physiol. 34:147-202 https://doi.org/10.1016/S0065-2911(08)60029-6
  21. Yamada, M., Sakuraba, S., Shibata, K., Taguchi, G, Inatomi, S., Okazaki, M. and Shimosaka, M. 2006. Isolation and analysis of genes specifically expressed during fruiting body development in the basidiomycete FIammulina velutipes by fluorescence differential display. FEMS Microbiol. Lett. 254:165-172 https://doi.org/10.1111/j.1574-6968.2005.00023.x