Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Kinetic Study of Organic Acid Formations and Growth of Anaerobiospirillum succiniciproducens During Continuous Cultures

  • Lee, Pyung-Cheon (Department of Molecular Science and Technology, Ajou University) ;
  • Lee, Sang-Yup (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Chang, Ho-Nam (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology)
  • Published : 2009.11.30
Download PDF
⟨ Previous Next ⟩

Abstract

Succinic acid-producing Anaerohiospirillum succinkiproducens was anaerobically grown in glucose-fed continuous cultures using glucose as a carbon source, and the metabolic flexibility of A. succiniciproducens in response to varying glucose concentrations and dilution rates was examined Both succinic acid (SA) and acetic acid (AA) formation was growth-associated, and their growth-rate-related coefficients ($K_{SA/X}$, $K_{AA/X}$) and nongrowth-rate-related coefficients ($K'_{SA/X}$, $K'_{AA/X}$) were slightly influenced by glucose concentrations. A high glucose concentration (38 g/l) and high growth rate ($0.63\;h^{-1}$) did not induce by-product formation.

Keywords

References

  1. Davis, C. P., D. Cleven, J. Brown, and E. Balish. 1976. Anaerobiospirillum, a new genus of spiral-shaped bacteria. Int. J. Syst. Bacteriol. 26: 498-504 https://doi.org/10.1099/00207713-26-4-498
  2. Guettler, M. V., D. Rumler, and M. K. Jain. 1999. Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen, Int. J. Syst. Bacteriol. 49: 207-216 https://doi.org/10.1099/00207713-49-1-207
  3. Guillouet, S. and J.M. Engasser. 1995. Growht of Corynebacterium glutamicum in glucose-limited continuous cultures under high osmotic pressure. Influence of growth rate on the intracellular accumulation of proline, glutamate and trehalose. Appl. Microbiol. Biotechnol. 44: 496-500 https://doi.org/10.1007/BF00169950
  4. Klein, C. J. L., L. Olsson, and J. Nielsen. 1998. Glucose control in Saccharomyces cerevisiae: The role of MIGI in metabolic functions. Microbiology 144: 13-24 https://doi.org/10.1099/00221287-144-1-13
  5. Kleman, G. L. and W. R. Strohl. 1994. Acetate metabolism by Escherichia-coli in high-cell-density fermentation. Appl. Environ. Microbiol. 60: 3952-3958
  6. Landucci, R., B. Goodman, and C. Wyman. 1994. Methodology for evaluating the economics of biologically producing chemicals and materials from alternative feedstocks. Appl. Biochem. Biotechnol. 45-6: 677-696
  7. Lee, P. C., S. Y. Lee, and H. N. Chang. 2008. Cell recycled culture of succinic acid-producing Anaerobiospirillum succiniciproducens using an internal membrane filtration system. J. Microbiol. Biotechnol. 18: 1252-1256
  8. Lee, P. C., S. Y. Lee, and H. N. Chang. 2008. Succinic acid production by Anaerobiospirillum succiniciproducens ATCC 29305 growing on galactose, galactose/glucose, and galactose/lactose. J. Microbiol. Biotechnol. 18: 1792-1796 https://doi.org/10.4014/jmb.0800.129
  9. Lee, P. C., S. Y. Lee, S. H. Hong, and H. N. Chang. 2002. Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E, from bovine rumen. Appl. Microbiol. Biotechnol. 58: 663-668 https://doi.org/10.1007/s00253-002-0935-6
  10. Lee, P. C., S. Y. Lee, S. H. Hong, H. N. Chang, and S. C. Park. 2003. Biological conversion of wood hydrolysate to succinic acid by Anaerobiospirillum succiniciproducens. Biotechnol. Lett. 25: 111-114 https://doi.org/10.1023/A:1021907116361
  11. Lee, P. C., W. GLee, S. Kwon, S. Y. Lee, and H. N. Chang. 2000. Batch and continuous cultivation of Anaerobiospirillum succiniciproducens for the production of succinic acid from whey. Appl. Microbiol. Biotechnol. 54: 23-27 https://doi.org/10.1007/s002530000331
  12. Lee, P. C., W. GLee, S. Kwon, S. Y. Lee, and H. N. Chang. 1999. Succinic acid production by Anaerobiospirillum succiniciproducens: Effects of the H-2/$CO_2$ supply and glucose concentration. Enzyme Microb. Technol. 24: 549-554 https://doi.org/10.1016/S0141-0229(98)00156-2
  13. Lee, P. C., W. G. Lee, S. Y. Lee, and H. N. Chang. 1999. Effects of medium components on the growth of Anaerobiospirillum succiniciproducens and succinic acid production. Process Biochem. 35: 49-55 https://doi.org/10.1016/S0032-9592(99)00031-X
  14. Lee, P. C., W. G. Lee, S. Y. Lee, and H. N. Chang. 2001. Succinic acid production with reduced by-product formation in the fermentation of Anaerobiospirillum succiniciproducens using glycerol as a carbon source. Biotechnol. Bioeng. 72: 41-48 https://doi.org/10.1002/1097-0290(20010105)72:1<41::AID-BIT6>3.0.CO;2-N
  15. Lee, S. Y, J. M. Kim, H. Song, J. W. Lee, T. Y. Kim, and Y. S. Jang. 2008. From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens. Appl. Microbiol. Biotechnol. 79: 11-22 https://doi.org/10.1007/s00253-008-1424-3
  16. Lin, S. K. C., C. Du, A. Koutinas, R. Wang, and C. Webb. 2008. Substrate and product inhibition kinetics in succinic acid production by Actinobacillus succinogenes. Biochem. Eng. J. 41: 128-135 https://doi.org/10.1016/j.bej.2008.03.013
  17. McKinlay, J. B., C. Vieille, and J. G. Zeikus. 2007. Prospects for a bio-based succinate industry. Appl. Microbiol. Biotechnol. 76: 727-740 https://doi.org/10.1007/s00253-007-1057-y
  18. Meynial-Salles, I., S. Dorotyn, and P. Soucaille. 2008. A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity. Biotechnol. Bioeng. 99: 129-135 https://doi.org/10.1002/bit.21521
  19. Nghiem, N. P., B. H. Davison, J. E. Thompson, B. E. Suttle, and G. R. Richardson. 1996. The effect of biotin on the production of succinic acid by Anaerobiospirillum succiniciproducens. Appl. Biochem. Biotechnol. 57-8: 633-638
  20. Okino, S., R. Noburyu, M. Suda, T. Jojima, M. Inui, and H. Yukawa. 2008. An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain. Appl. Microbiol. Biotechnol. 81: 459-464 https://doi.org/10.1007/s00253-008-1668-y
  21. Oner, M. D., L. E. Erickson, and S. Yang. 1988. Bioenergetics and product yields, p. 293-323. In L. E. Erickson and Y. Fung (eds.). Handbook on Anaerobic Fermentation, 2nd Ed. Marcel Dekker Inc., NY
  22. Russell, J. B. and G. M. Cook. 1995. Energetics of bacterial growth: Balance of anabolic and catabolic reactions. Microbiol. Rev. 59: 48-62
  23. Samuelov, N. S., R. Lamed, S. Lowe, and I. G Zeikus. 1991. Influence of $CO_2-HCO_3-$ levels and pH on growth, succinate production, and enzyme activities of Anaerobiospirillum succiniciproducens. Appl. Environ. Microbiol. 57: 3013-3019
  24. Sanchez, A. M., G. N. Bennett, and K. Y. San. 2005. Efficient succinic acid production from glucose through overexpression of pyruvate carboxylase in an Escherichia coli alcohol dehydrogenase and lactate dehydrogenase mutant. Biotechnol. Progress 21: 358-365 https://doi.org/10.1021/bp049676e
  25. Shene, C. and S. Bravo. 2007. Whey fermentation by Lactobacillus delbrueckii subsp. bulgaricus for exopolysaccharide production in continuous culture. Enz. Microbial. Technol. 40: 1578-1584 https://doi.org/10.1016/j.enzmictec.2006.11.003
  26. VanderWerf, M. J., M. V. Guettler,M. K. Jain, and J. G. Zeikus. 1997. Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z. Arch. Microbiol. 167: 332-342 https://doi.org/10.1007/s002030050452
  27. Zeikus, J. G. 1980. Chemical and fuel production by anaerobicbacteria. Annu. Rev. Microbiol. 34: 423-464 https://doi.org/10.1146/annurev.mi.34.100180.002231

Cited by

  1. Continuous succinic acid production by Actinobacillus succinogenes on xylose-enriched hydrolysate vol.8, pp.None, 2015, https://doi.org/10.1186/s13068-015-0363-3
  2. Succinic acid production on xylose-enriched biorefinery streams by Actinobacillus succinogenes in batch fermentation vol.9, pp.None, 2016, https://doi.org/10.1186/s13068-016-0425-1
  3. Technologies for Biogas Upgrading to Biomethane: A Review vol.6, pp.4, 2009, https://doi.org/10.3390/bioengineering6040092