Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Anti-sclerotic Effect of Cinnamomi Ramulus Via Suppression of MMP-9 Activity and Migration of TNF-$\alpha$-induced HASMC

인간대동맥평활근의 유주능 및 기질금속단백분해효소의 억제를 통한 계지의 항동맥경화능

  • Kim, Jai-Eun (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Lee, Chang-Sup (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Choi, Sung-Kyu (Department of Pathology, College of Oriental Medicine, Dongguk University) ;
  • Choi, Dall-Yeong (Department of Pathology, College of Oriental Medicine, Dongguk University)
  • 김재은 (동국대학교 한의과대학 병리학교실) ;
  • 이창섭 (동국대학교 한의과대학 병리학교실) ;
  • 최성규 (동국대학교 한의과대학 병리학교실) ;
  • 최달영 (동국대학교 한의과대학 병리학교실)
  • Published : 2009.10.25
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Abstract

Proliferation of vascular smooth muscle cell(VSMC) is one of the key features in onset of atherosclerosis and restenosis after vascular surgery such as stent implant. Atherosclerotic plaques are usually composed of collagen, elatsin and smooth muscle cells. Release of matrix metalloproteinases(MMPs) is considered to have correlation with development of atherosclerotic plaques. Based on the hypothesis that MMP inhibition would be helpful in the treatment of atherosclerosis, we investigated inhibition of MMP activity and migration of TNF-$\alpha$-induced human aortic smooth muscle cell(HASMC) by Cinnamomi Ramulus(CC). The result from gelatin zymography showed that CC inhibited MMP-9 activity in a dose-dependent manner. In addition, CC considerably inhibited the migration of HASMC induced by TNF-$\alpha$, while it showed little cytotoxic effect on HASMC. These results suggest that CC can be a potential anti-atherosclerotic agent through inhibition of MMP-9 activity and SMC migration.

Keywords

References

  1. Ross, R. The Pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 362: 801-809, 1993 https://doi.org/10.1038/362801a0
  2. Newby, A.C,, Zaltsman, A.B. Molecular mechanisms in intimal hyperplasia. J. Pathol. 190: 300-309, 2001 https://doi.org/10.1002/(SICI)1096-9896(200002)190:3<300::AID-PATH596>3.0.CO;2-I
  3. Cho, A., Reidy, M.A. Matrix metalloproteinase-9 is necessary for the regulation of smooth muscle cell replication and migration after arterial injury. Circ Res. 91: 845-851, 2002 https://doi.org/10.1161/01.RES.0000040420.17366.2E
  4. 김호철. 한약약리학. 서울, 집문당, pp 66-67, 2001
  5. Park, H.J., Lee, J.S., Lee, J.D., et al. The anti-inflammatory effect of Cinnamomi Ramulus. J. Kor Orient Med 26: 140-151, 2005
  6. Wu, S.J., Ng, L.T., Lin, C.C. Cinnamaldehyde-induced apoptosis in human PLC/PRF/5 cells through activation of the proapoptotic Bcl-2 family proteins and MAPK pathway Life Sciences 77: 938-951, 2005 https://doi.org/10.1016/j.lfs.2005.02.005
  7. 대한병리학회, 병리학 (제5판). 서울, 고문사, pp 324-325, 2003
  8. Ngo, D.T., Sverdlov, A.L., Willoughby, S.R., Nightingale, A.K., Chirkov, Y.Y., McNeil, J.J., Horowitz, J.D. Determinants of occurrence of aortic sclerosis in an aging population. JACC Cardiovasc Imaging. 2(8):919-927, 2009 https://doi.org/10.1016/j.jcmg.2009.03.016
  9. Rouis, M. Matrix metalloproteinase : a potential therapeutic target in atherosclerosis, Curr Drug Targets Cardiovasc Haematol Discord. 5(6):541-548, 2005 https://doi.org/10.2174/156800605774961979
  10. Mason, D.P., Kenagy, R.D., Hasenstab, D., Bowen-Pope, D.F., Seifert, R.A., Coats, S., Hawkings, S.M., Clowes, A.W. Matrix metalloproteinase-9 overexpression enhances vascular smooth muscle cell migration and alters remodeling in the injured rat carotid artery. Circ Res. 85(12):1179-1185, 1999 https://doi.org/10.1161/01.RES.85.12.1179
  11. Lovdhal, C., Thyberg, J., Hultgardh Nilsson, A. The syntehtic metalloproteinase inhibitor batimastat suppresses injury-induced phosphorylation of MAPkinase ERK1/2 and phenotypic modification of arterial smooth muscle cells in vitro. J Vasc Res. 37(5):345-354, 2000 https://doi.org/10.1159/000025750
  12. Porter, K.E., Turner, N.A. Statins for the prevention of vein graft stenosis: a role for inhibitor of matrix metalloproteinase-9. Biochem Soc Trans. 30(2):120-126, 2002 https://doi.org/10.1042/BST0300120
  13. Maeda, K., Kuzuya, M., Cheng, X.W., Asai, T., Kanda, S., Tamaya-Mori, N., Sasaki, T., Shibata, T., Iguchi, A. Green tea catechins inhibit the cultured smooth muscle cell invasion through the basement barrier. Atherosclerosis. 166(1):23-30, 2003 https://doi.org/10.1016/S0021-9150(02)00302-7
  14. 전국한의과대학공동교과편찬위원회. 본초학. 서울, 영림사, pp 156-157, 2004
  15. Park, H.J., Lee, J.S., Lee, J.D., et al. The anti-inflammatory effect of Cinnamomi Ramulus. J. Kor Orient Med 26: 140-151, 2005
  16. Jeong, H.W., Han, D.C., Son, K.H., Han, M.Y., Lim, J.S., Ha, J.H., Lee, C.W., Kim, H.M., Kim, H.C., Kwon, B.M. Antitumor effect of the cinnamaldeyde derivative CB403 through the arrest of cell cycle progression in the G2/M phase. Biochemical pharmacology 65(8):1343-1350, 2003 https://doi.org/10.1016/S0006-2952(03)00038-8
  17. Lee, W.C., Lee, H.S., Lee, W.J., Ban, O.J., Lee, Y.S., Yoo, S.H., Jung, K.J., Moon, C.D., Oh, W.K., Hong, T.J. 2-Hydroxycinnamaldeyhe inhibits SW620 Colon Cancer Cell growth through AP-1 inactivation. Journal of pharmacological sciences 104(1):19-28, 2007 https://doi.org/10.1254/jphs.FP0061204