Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effects of Samul-tang on Cell Death Inducded by Oxidative Stress in C6 Glial Cell

사물탕이 산화적 스트레스에 의하여 유발되는 신경세포의 세포 사멸에 미치는 보호효과

  • Published : 2009.10.25
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Abstract

Samul-tang (SMT), which was firstly described in (Hwajegukbang) Song dynasty, is well known remedy for blood diseases in Oriental medicine. SMT is traditional herbal-remedy composed of Rehmanniae Radix Preparat, Angelicae Gigantis Radix, Cnidii Rhizoma and Paeoniae Radix. Recently, SMT has known to have anti-oxidative action. However, the reports on anti-oxidantic action in neuroglial cells are rare. In addition, the exact mechanisms are unclear. For these reasons, we investigated the protective effects of SMT on cell death induced by oxidative stress using C6 glioma cells. In our results, SMT accelerated proliferation rates of C6 cells in vitro. In addition, levels of LDH release induced by oxidative stress were lowered by treatment with SMT. Finally, protective effects on cell death induced by chemicals such as paraquat and rotenone were observed. In conclusion, these results suggest the possibility to protect brain cell or neuronal cell from damage induced by oxidative stress.

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References

  1. 윤용갑. 東醫處方과 方劑解說. 서울, 의성당, pp 163-169, 1998
  2. 陣偉, 路一平. 方劑學. 上海, 上海中醫學院出版社, pp 205-208, 1993
  3. 안희덕. 四物湯의 항암제 부작용 억제에 관한 실험적 연구. 동의병리학회지 19(2):341-359, 1995
  4. 이상곤, 권영규, 김광중, 김완희. 사물탕과 육미지황탕이 난소적출로 유도된 백서의 골다공증에 미치는 영향. 제한동의학술원논문집, 1(1):31-48, 1995
  5. 은재순, 유동화, 권진, 오찬호. 사물탕이 L1210 세포 이식 및 항암제를 투여한 마우스의 면역세포에 미치는 영향. 생약학회지 29(2):110-119, 1998
  6. 전훈, 은재순, 송정모. 사물탕이 복강 Macrophage 의 탐식능에 미치는 영향. 대한본초학회지 14(2):75-80, 1999
  7. 이기태, 유병찬, 김윤식, 설인찬. 사물탕(四物湯)이 손상된 말초신경섬유 재생에 미치는 효과에 대한 사전 연구. 한의학논문집, 14(2):107-112, 2005
  8. 조기용, 유동열. 사군자탕 및 사물탕이 인체파동과 활성산소에 미치는 영향에 관한 실험적 연구. 한의학논문집, 9(1):305-317, 2000
  9. 최미애, 김미림, 박찬성. 사물탕 재료 추출물의 항균 및 항산화능. 한국식품조리과학회지 24(1):52-58, 2008
  10. Lee, S.O., Kim, M.J., Kim, D.K., Choi, H.J. Antioxidative activities of temperature-stepwise water extracts from Inonotus obliquns. Korean J. Food Sci. Nutr. 34: 139-147, 2005 https://doi.org/10.3746/jkfn.2005.34.2.139
  11. 조숙현, 최용조, 노치웅, 최철웅, 김덕송, 조성환. 대나무수액의 활성산소 소거활성과 세포독성. 한국식품저장유통학회지 15(1):105-110, 2008
  12. PaPa, S., Skulachev, V.P. Reactive oxygen species, mitochondria, apoptosis and aging. Mol. Cell. Biochem. 174: 305-319, 1997 https://doi.org/10.1023/A:1006873518427
  13. Slavic, M., Appiah, I., Nikolic-Kokic, A., Radojicic, R., Jones, D.R., Spasic, M.B., Milovanovic, S., Blagojevic, D. The anti-oxidative defence system in the isolated rat uterus during spontaneous rhythmic activity. Acta Physiol Hung. 93(4):335-339, 2006 https://doi.org/10.1556/APhysiol.93.2006.4.9
  14. Szuster-Ciesielska, A., Daniluk, J. and Kandefr-Szerszen, M. Alcohol-related cirrosis with pancreatitis. The role of oxidative stress in the progression of the disease. Arch. Immunol. Ther. Exp. 49: 19-22, 2001
  15. Sranely Mainzen Princem, P. and Menon, V.P. Antioxidant action of Tinospora cordifolia root extract in alloxan diabetic rats. Phytother. Res. 15, 213-217, 2001 https://doi.org/10.1002/ptr.707
  16. Mari, M., Seiji, K., Kenji, F., Airo, T. and Toshimasa, N. Evaluation of the estrogenic activities of some pesticides and their combinations using MtT/Se cell proliferation assay. International Journal of Hygiene and Environmental Health. 209(5):413-421, 2006 https://doi.org/10.1016/j.ijheh.2006.04.004
  17. 전국한의과대학교수 공저. 본초학. 서울, 영림사, pp 450, 632-637, 2004
  18. 안상원, 이철완. 숙지황 (熟地黃)과 육미지황탕 (六味地黃湯)이 노화과정 흰쥐에서의 항산화 기전에 미치는 영향. 한의학논문집, 8(1):593-623, 1999
  19. 조수인. 흰쥐 신장 조직 손상에 대한 숙지황의 항산화 효과. 대한본초학회지 18(4):119-126, 2003
  20. 장문석, 양웅모, 유태원, 김도림, 박은화, 고은빛, 최문정, 김휴영, 오지훈, 심경준, 윤지원, 박성. 숙지황(熟地黃)이 남성생식세포 GC-1의 항산화에 미치는 영향. 대한본초학회지 22(4):81-86, 2007
  21. 안준철, 문진영, 임종국. 당귀 (當歸) 약침액의 항산화 효능에 관한 연구. 대한침구학회지 13(2):254-262, 1996
  22. 임창수, 김갑성. 작약 약침액의 항산화 효능에 관한 연구. 대한침구학회지 14(2):191-198, 1997
  23. Corl, M.M. Antioxidant activity of tocopherol and ascorbylpalmitate and their mode of action. JAOCS. 51: 321-325, 1974 https://doi.org/10.1007/BF02633006
  24. Coleman, M.D., Fermandes, S. and Khanderia, L.A. A preliminary evaluation of a novel method to monitor a triple antioxidant combination in diabetic volunteers using in vitro methahemoglobin formation. Environ. Toxicol. Pharmacol. 14: 69-75, 2003 https://doi.org/10.1016/S1382-6689(03)00027-9
  25. Wills, B.K., Aks, S., Maloney, G.E., Rhee, J., Brand, R., Sekosan, M. The effect of amifostine, a cytoprotective agent, on paraquat toxicity in mice. J Med Toxicol. 3(1):1-6, 2007 https://doi.org/10.1007/BF03161031
  26. Krasowska, A., Sigler, K. Cell-protective and antioxidant activity of two groups of synthetic amphiphilic compounds-phenolics and amine N-oxides. Folia Microbiol (Praha). 52(6):585-592, 2007 https://doi.org/10.1007/BF02932187
  27. Mullens, W., Abrahams, Z., Francis, G.S., Skouri, H.N., Starling, R.C., Young, J.B., Taylor, D.O., Tang, W.H. Sodium nitroprusside for advanced low-output heart failure. J Am Coll Cardiol. 52(3):200-207, 2008 https://doi.org/10.1016/j.jacc.2008.02.083
  28. Molina-Jimenez, M.F., Sanchez-Reus, M.I., Andres, D., Cascales, M., Benedi, J. Neuroprotective effect of fraxetin and myricetin against rotenone-induced apoptosis in neuroblastoma cells. Brain Res. 29, 1009(1-2):9-16, 2004 https://doi.org/10.1016/j.brainres.2004.02.065
  29. Zhang, J.G., Nicholls-Grzemski, F.A., Tirmenstein, M.A., Fariss, M.W. Vitamin E succinate protects hepatocytes against the toxic effect of reactive oxygen species generated at mitochondrial complexes I and III by alkylating agents. Chem Biol Interact. 138(3):267-284, 2001 https://doi.org/10.1016/S0009-2797(01)00278-2