Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Real-Time Glutamate Release in Rat Striatum of 11-Vessel-Occlusion Ischemia Model Treated with Acupuncture

11개 혈관 차단법을 통한 중증 뇌경색 모델에서 뇌손상 측정과 침치료 효과 실시간 분석

  • Yin, Chang-Shik (Acupuncture and Meridian Science Research Center, Kyung Hee University) ;
  • Choi, Seok-Keun (Department of Neurosurgery, College of Meridian, Kyung Hee University) ;
  • Lee, Gi-Ja (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Kyung Hee University) ;
  • Eo, Yun-Hye (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Kyung Hee University) ;
  • Kim, Bum-Shik (Healthcare Industry Research Institute) ;
  • Oh, Berm-Seok (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Program of Medical Engineering, Kyung Hee University) ;
  • Lim, Ji-Eun (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Kyung Hee University) ;
  • Lee, Hye-Jung (Acupuncture and Meridian Science Research Center, Department of Meridian & Acupuncture College of Oriental Meridian, Kyung Hee University) ;
  • Park, Hun-Kuk (Department of Biomedical Engineering, College of Meridian, Healthcare Industry Research Institute, Program of Medical Engineering, Kyung Hee University)
  • 인창식 (경희대학교 침구경락과학연구센터) ;
  • 최석근 (의과대학 신경외과학교실) ;
  • 이기자 (경희대학교 의과대학 의공학교실, 의료산업연구원) ;
  • 어윤혜 (경희대학교 의과대학 의공학교실, 의료산업연구원) ;
  • 김범식 (의료산업연구원) ;
  • 오범석 (경희대학교 의과대학 의공학교실, 의료산업연구원, 대학원 생체의과학 협동과정) ;
  • 임지은 (경희대학교 의과대학 의공학교실, 의료산업연구원) ;
  • 이혜정 (경희대학교 침구경락과학연구센터, 한의과대학 경혈학교실) ;
  • 박헌국 (경희대학교 의과대학 의공학교실, 의료산업연구원, 대학원 생체의과학 협동과정)
  • Published : 2008.08.25
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Abstract

Acupuncture has long been contended to be effective in an ischemic stroke. A real-time monitoring of glutamate, an excitotoxin in the process of ischemic neuronal damage, in the striatum is tried in a rat model of global ischemia. Global ischemia was induced by the 11 vessel occlusion method for 10 minutes, during which acupuncture stimulation on GB34 and GB39 points was executed. Glutamate release in the rat striatum was monitored 256 times per second using real-time amperometric biosensor. Real time measurement data of 10 minutes prior to the induction of ischemia served as baseline data. Data acquisition continued for 30 minutes after the initiation of reperfusion. Peak concentration of glutamate release along with incidentally measured EEG and cerebral blood flow was compared between cases with and without acupuncture stimulation. Peak concentration of glutamate lowered when acupuncture stimulation was executed. A real time monitoring system of 11 vessel-occlusion induced global ischemia model was successfully established. The effect by acupuncture on acute global ischemia was successfully observed in this real-time monitoring setting, which may be one of the neuroprotective mechanism of acupuncture.

Keywords

References

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